Skip to main content
European Commission logo
Deutsch Deutsch
CORDIS - Forschungsergebnisse der EU
CORDIS
CORDIS Web 30th anniversary CORDIS Web 30th anniversary
Inhalt archiviert am 2024-06-18

Visualizing Functional Structural Dynamics of Membrane Protein Transporters

Ziel

Membrane protein transporters catalyze fundamental physiological processes in all kingdoms of life. A schematic view of membrane transport is emerging from a growing number of crystal structures. However, several fundamental features of membrane protein transport are still evasive, such as how the protein transition between states, what triggers these structural rearrangements, and how the release of energy is coupled to the transport event. The proposed research will build an interdisciplinary platform, which uses molecular biology, biophysical and computational techniques, to characterize membrane protein transport over the full spatio-temporal scale. Molecular dynamics (MD) simulations will capture high-resolution dynamics on the nano-to-microsecond timescale, which will identify communicative salt-bridge/H-bond networks, putative transport pathways, and the energetics involved in substrate transport. Predictions from the simulations will be verified by in vitro characterization of mutagenic phenotypes. Time-resolved wide-angle X-ray scattering (TR-WAXS) will monitor the micro-to-millisecond, large-scale structural rearrangements, which are out of reach for the MD simulations. The proposed platform for characterization of membrane protein transporters will be developed using a copper-transporting P-type ATPase (CopA) as the initial model system. The P-type ATPase family is divided into types I-V, depending on the transported substrate. CopA is the first crystal structure from the type-I ATPases, making it a particularly exciting model system. The applicants’ preliminary molecular dynamics (MD) simulations and the CopA transmembrane architecture strongly indicate a unique mode of transport in type-I ATPases. Hence, by means of visualization of copper transport, this proposal enables the development of an interdisciplinary European platform for multifaceted characterization of membrane transport that will be extendable to a range of membrane protein targets.

Wissenschaftliches Gebiet

CORDIS klassifiziert Projekte mit EuroSciVoc, einer mehrsprachigen Taxonomie der Wissenschaftsbereiche, durch einen halbautomatischen Prozess, der auf Verfahren der Verarbeitung natürlicher Sprache beruht.

Aufforderung zur Vorschlagseinreichung

FP7-PEOPLE-2013-CIG
Andere Projekte für diesen Aufruf anzeigen

Koordinator

KUNGLIGA TEKNISKA HOEGSKOLAN
EU-Beitrag
€ 100 000,00
Adresse
BRINELLVAGEN 8
100 44 Stockholm
Schweden

Auf der Karte ansehen

Region
Östra Sverige Stockholm Stockholms län
Aktivitätstyp
Higher or Secondary Education Establishments
Links
Gesamtkosten
Keine Daten