Single-molecule visualization of transcription dynamics to understand regulatory mechanisms of transcriptional bursting and its effects on cellular fitness

Project Information

BURSTREG

Grant agreement ID: 755695

DOI 10.3030/755695

Project closed

EC signature date 6 September 2017

Start date 1 January 2018

End date 31 December 2022

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 1 950 775,00

EU contribution € 1 950 775,00

Coordinated by

STICHTING HET NEDERLANDS KANKER INSTITUUT-ANTONI VAN LEEUWENHOEK ZIEKENHUIS
Netherlands

Publications

Peer reviewed articles (8)

Optimized protocol for single-molecule RNA FISH to visualize gene expression in S. cerevisiae

Author(s): Heta P Patel, Ineke Brouwer, Tineke L Lenstra
Published in: STAR Protocols, 2021, ISSN 2666-1667
Publisher: Elsevier
DOI: 10.1016/j.xpro.2021.100647

The Hda1 histone deacetylase limits divergent non-coding transcription and restricts transcription initiation frequency

Author(s): Uthra Gowthaman, Maxim Ivanov, Isabel Schwarz, Heta P Patel, Niels A Müller, Desiré García-Pichardo, Tineke L Lenstra, Sebastian Marquardt
Published in: EMBO Journal, Issue (2021)40:e108903, 2021, ISSN 1460-2075
Publisher: EMBO press
DOI: 10.15252/embj.2021108903

Single-Molecule Fluorescence Imaging in Living Saccharomyces cerevisiae Cells

Author(s): Ineke Brouwer; Joseph Victor Willem Meeussen; Heta Piyush Patel; Wim Pomp; Tineke L. Lenstra
Published in: STAR Protocols, Vol 1, Iss 3, Pp 100142- (2020), Issue 1, 2020, ISSN 2666-1667
Publisher: Elsevier
DOI: 10.1016/j.xpro.2020.100142

Transcription-replication coordination revealed in single live cells

Author(s): Ioannis Tsirkas; Daniel Dovrat; Manikandan Thangaraj; Ineke Brouwer; Amit Cohen; Zohar Paleiov; Michael M Meijler; Tineke Lenstra; Amir Aharoni
Published in: Nucleic Acids Research, Issue 2, 2022, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkac069

Live‐cell imaging reveals the interplay between transcription factors, nucleosomes, and bursting

Author(s): Benjamin T Donovan, Anh Huynh, David A Ball, Heta P Patel, Michael G Poirier, Daniel R Larson, Matthew L Ferguson, Tineke L Lenstra
Published in: The EMBO Journal, Issue 38/12, 2019, Page(s) e100809, ISSN 0261-4189
Publisher: Nature Publishing Group
DOI: 10.15252/embj.2018100809

Visualizing transcription: key to understanding gene expression dynamics

Author(s): Ineke Brouwer, Tineke L Lenstra
Published in: Current Opinion in Chemical Biology, Issue 51, 2019, Page(s) 122-129, ISSN 1367-5931
Publisher: Elsevier BV
DOI: 10.1016/j.cbpa.2019.05.031

Quantifying how post-transcriptional noise and gene copy number variation bias transcriptional parameter inference from mRNA distributions

Author(s): Heta P Patel; Xiaoming Fu; Stefano Coppola; Libin Xu; Zhixing Cao; Tineke L Lenstra; Ramon Grima
Published in: Elife, Issue 1, 2022, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.82493

Following the tracks: How transcription factor binding dynamics control transcription.

Author(s): Wim J de Jonge, Heta P Patel, Joseph V W Meeussen, Tineke L Lenstra
Published in: Biophysical Journal, 2022, ISSN 1542-0086
Publisher: Elsevier
DOI: 10.1016/j.bpj.2022.03.026