Periodic Reporting for period 1 - ART-BONE (Manufacturing of Artificial Bone for Repair and Regeneration of Large Osseous Defects)
Okres sprawozdawczy: 2018-09-03 do 2020-09-02
The goal of the ART-BONE project was to develop and evaluate a new class of fourth generation biomaterials for bone repair. To reach this goal, the fellow achieved three different objectives: the fellow designed (objective 1), characterized (objective 2) and evaluated (objective 3) bone-mimetic biomaterials in the form of inorganic-organic composite materials. The fellow has also compared these bone-mimetic biomaterials with traditional biomaterials for bone repair and regeneration.
As a conclusion, this action provides a new mechanistic understanding of the physicochemical processes underlying osteogenesis. Further, this action has had a major impact in the researcher’s career since it allowed him to secure a permanent academic position within less than a year after the end of the action.
Objective 2. The bone-mimetic composite materials prepared in objective 1 were characterized using a set of physical characterization techniques, including solid-state Nuclear Magnetic Resonance (NMR) spectroscopy and scanning helium ion microscopy (SHIM).
Objective 3. The bone repair ability of the bone-mimetic composite materials fabricated in objective 1 and characterized in objective 2 were evaluated and compared to that of composite materials made of commercially available hydroxyapatites in preclinical studies. To this end, a classical bioengineering approach was applied: human bone marrow mesenchymal stem cells (hMSCs) were cultured on the composite materials over a period up to 21 days. Cells’ proliferation and viability were examined using DNA quantification. Cells’ osteogenic differentiation was assessed in terms of alkaline phosphatase (ALP) activity, collagen production and calcium phosphate deposition, but also in terms of osteogenic genes expression levels.
The work of the fellow through the ART-BONE project has been translated into five peer-reviewed scientific publications to date, with two more publications which are currently under preparation. The fellow has also presented the outcomes of the project in one national conference and in one international conference.