Descrizione del progetto
Produrre 100 composti ad alto valore aggiunto dalla via dello shikimato
La sintesi chimica è spesso difficile e di solito si traduce in sottoprodotti indesiderati. Sebbene la natura possa fornire molti prodotti, le loro concentrazioni molto basse rendono l’estrazione costosa. Al contrario, la produzione microbica è un processo consolidato per i composti prodotti dall’ospite o che hanno percorsi biosintetici completamente caratterizzati. Sono tuttavia necessari importanti screening per la produzione efficiente di composti target derivati dalla natura in applicazioni industriali. In quest’ottica, il progetto SHIKIFACTORY100, finanziato dall’UE, mira a produrre oltre 100 composti ad alto valore aggiunto dalla via dello shikimato, un crocevia nel metabolismo cellulare, sviluppando un telaio shikimato ottimizzato. Esso proporrà e implementerà inoltre nuovi percorsi biosintetici che esplorano la promiscuità enzimatica in modo da raccomandare nuovi percorsi per la produzione di composti noti e di nuova concezione.
Obiettivo
Chemical synthesis is often difficult and may be subject to unclear mechanisms, frequently producing undesired by-products, while the complexity of some compounds may rendered them virtually unattainable. Nature can provide a wealth of products, however typically at very low concentrations, which makes their extraction process expensive. Conversely, microbial production is a well-established process for compounds naturally produced by the hosts or for which there are fully characterised biosynthetic pathways. However, relying on nature’s diversity as a provider for industrial applications typically requires major screenings for pathways, genes and optimisation of cell factories for the efficient production of the target compounds.
SHIKIFACTORY100 project aims towards the production of a universe of more than 100 high-added value compounds from the shikimate pathway, a hub in cell metabolism, through the development of an optimised shikimate chassis) and the proposal and implementation of novel biosynthetic routes exploring enzyme promiscuity to propose new pathways for the production of known and newly designed compounds. To champion SHIKIFACTORY100 project a consortium with some of the most relevant players in Europe has been assembled, covering research leading institutions in computational design, rapid prototyping and in vivo implementation of pathways and cell factories. As such, six world-leading research partners, four biotechnology SMEs with complementary focus areas and one large industrial company have been integrated. The research partners will be responsible for novel methods for pathway discovery and compound design, collaborating with the SMEs in the implementation and optimisation of the chassis, while SMEs and industrial partners will lead the assessment of the products and the exploitation of the results for different application areas. The industrial partner will be responsible for the validation of the production.
Campo scientifico
Parole chiave
Programma(i)
Argomento(i)
Invito a presentare proposte
Vedi altri progetti per questo bandoBando secondario
H2020-NMBP-BIO-2018-two-stage
Meccanismo di finanziamento
RIA - Research and Innovation actionCoordinatore
4715 387 Braga
Portogallo
L’organizzazione si è definita una PMI (piccola e media impresa) al momento della firma dell’accordo di sovvenzione.