Super-sensitive detection of Alzheimer’s disease biomarkers in plasma by an innovative droplet split-and-stack approach

Projektinformationen

SensApp

ID Finanzhilfevereinbarung: 829104

DOI

10.3030/829104

Projekt abgeschlossen

EK-Unterschriftsdatum 17 Dezember 2018

Startdatum 1 Januar 2019

Enddatum 30 Juni 2022

Finanziert unter

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Gesamtkosten

€ 3 287 562,50

EU-Beitrag

€ 3 287 562,50

Koordiniert durch

CONSIGLIO NAZIONALE DELLE RICERCHE
Italy

CORDIS bietet Links zu öffentlichen Ergebnissen und Veröffentlichungen von HORIZONT-Projekten.

Links zu Ergebnissen und Veröffentlichungen von RP7-Projekten sowie Links zu einigen Typen spezifischer Ergebnisse wie Datensätzen und Software werden dynamisch von OpenAIRE abgerufen.

Leistungen

Demonstrators, pilots, prototypes (3)

Integrated system for calibration and testing
First prototypes of climate chamber module and liquid handling module and specification for the automated system

Realization of the first prototypes of climate chamber module and liquid handling module and specification for the automated system

First prototype of the three individual layers for successive full integration

Realization of the first prototype of the three individual layers for successive full integration of the super-sensor.

Websites, patent fillings, videos etc. (3)

Logo and webpage

Realization of the logo of the Project and of a dedicated website with domain .eu in the address.

Flyers with project targets and first results and promotional movie

Flyers which describe the project targets and the first results achieved. Movie which explains the aims of the Project and its impact in order to disseminate as best as possible the research activities.

International Symposium

Organization of an International Symposium involving the main Universities and Research Centers and Companies working in the field of early diagnosis in neurodegenerative diseases but also on fields regarding microscale fluid handling and immunoreactions at sub-microliter levels.

Documents, reports (5)

Final plan for dissemination and exploitation

Report on the final plan for dissemination and exploitation of the results

Technical/scientific review meeting 1 documents

Document delivered as first report with a summary on the work in progress and on the use of resources including those regarding the dissemination activities

Report on sensitivity and specificity for tau

Report containing the data obtained for the sensitivity and the specificity of the supersensor for the biomarker tau

Report on sensitivity and specificity for Abeta1-42

Report containing the data concerning the results on the sensitivity and specificity of the supersensor for the biomarker Abeta142

Report on sensitivity and specificity for P-tau

Report document containing the data obtained for the sensitivity and the specificity of the supersensor for the biomarker Ptau

Veröffentlichungen

Peer reviewed articles (11)

High‐Rate Accumulation of Tiny Aqueous Droplets Using a Pyroelectrohydrodynamic Jet System

Autoren: Volodymyr Tkachenko, Reinhard Schwödiauer, Romina Rega, Simona Itri, Martina Mugnano, Danila del Giudice, Heidi Ottevaere, Yunfeng Nie, Pietro Ferraro, Simonetta Grilli
Veröffentlicht in: Advanced Engineering Materials, 2021, Seite(n) 2100756, ISSN 1438-1656
Herausgeber: John Wiley & Sons Ltd.
DOI: 10.1002/adem.202100756

Direct printing of gold nanospheres from colloidal solutions by pyro-electrohydrodynamic jet allows hypersensitive SERS sensing

Autoren: Marianna Pannico, Pellegrino Musto, Romina Rega, Veronica Vespini, Simonetta Grilli, Pietro Ferraro
Veröffentlicht in: Applied Surface Science, Ausgabe 531, 2020, Seite(n) 147393, ISSN 0169-4332
Herausgeber: Elsevier BV
DOI: 10.1016/j.apsusc.2020.147393

Miniaturized, high numerical aperture confocal fluorescence detection enhanced with pyroelectric droplet accumulation for sub-attomole analyte diagnosis

Autoren: YUNFENG NIE, UUSITALO SANNA, TEEMU SIPOLA, ANNUKKA KOKKONEN, INKA PÄKKILÄ, JUHA SUMEN, KATARIINA RAHKAMAA-TOLONEN, VOLODYMYR TKACHENKO, VERONICA VESPINI, SARA COPPOLA, PIETRO FERRARO, SIMONETTA GRILLI, HEIDI OTTEVAERE
Veröffentlicht in: Biomedical Optics Express, 2023, ISSN 2156-7085
Herausgeber: The Optical Society
DOI: 10.1364/boe.504757

High-accuracy characterization of pyroelectric materials: A noncontact method based on surface potential measurements

Autoren: Reinhard Schwödiauer, Simona Bauer-Gogonea, Martin Kaltenbrunner, Volodymyr Tkachenko, Simonetta Grilli
Veröffentlicht in: JOURNAL OF ADVANCED DIELECTRICS, 2023, ISSN 2010-1368
Herausgeber: World Scientific
DOI: 10.1142/s2010135x23410023

Rapid Formation of Self‐Supporting Polydimethylsiloxane Sheets with Periodic Clusters of Embedded Nickel Nanoparticles

Autoren: Romina Rega, Juan Francisco Muñoz Martinez, Vito Pagliarulo, Angela Longo, Mariano Palomba, Gianfranco Carotenuto, Pietro Ferraro, Simonetta Grilli
Veröffentlicht in: Advanced Materials Interfaces, 2021, Seite(n) 2002216, ISSN 2196-7350
Herausgeber: Wiley-VCH GmbH
DOI: 10.1002/admi.202002216

Cellular Uptake of Mildly Oxidized Nanographene for Drug-Delivery Applications

Autoren: M. Mugnano, G. C. Lama, R. Castaldo, V. Marchesano, F. Merola, D. del Giudice, A. Calabuig, G. Gentile, V. Ambrogi, P. Cerruti, P. Memmolo, V. Pagliarulo, P. Ferraro, S. Grilli
Veröffentlicht in: ACS Applied Nano Materials, Ausgabe 3/1, 2019, Seite(n) 428-439, ISSN 2574-0970
Herausgeber: ACS Publications
DOI: 10.1021/acsanm.9b02035

Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation

Autoren: Romina Rega, Martina Mugnano, Emilia Oleandro, Volodymyr Tkachenko, Danila del Giudice, Gianluca Bagnato, Pietro Ferraro, Simonetta Grilli, Sebastiano Gangemi
Veröffentlicht in: Sensors, Ausgabe 20/12, 2020, Seite(n) 3567, ISSN 1424-8220
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s20123567

Quantitative determination of rapid biomass formation on pyro-electrifiedpolymer sheets

Autoren: Emilia Oleandro, Romina Rega, Martina Mugnano, Filomena Nazzaro, Pietro Ferraro, Simonetta Grilli
Veröffentlicht in: Biofilm, Ausgabe 3, 2021, Seite(n) 100040, ISSN 2590-2075
Herausgeber: Elsevier
DOI: 10.1016/j.bioflm.2020.100040

Highly sensitive detection of the neurodegenerative biomarker Tau by using the concentration effect of the pyro-electrohydrodynamic jetting

Autoren: Concetta Di Natale, Sara Coppola, Veronica Vespini, Volodymyr Tkachenko, Simone Russo, Giuseppina Luciani, Giuseppe Vitiello, Francesca Ferranti, Silvia Mari, Pietro Ferraro, Pier Luca Maffettone, Simonetta Grilli
Veröffentlicht in: Biosensors and bioelectronics, 2024, ISSN 0956-5663
Herausgeber: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2024.116234

Pyro-Electrification of Freestanding Polymer Sheets: A New Tool for Cation-Free Manipulation of Cell Adhesion in vitro

Autoren: Romina Rega, Oriella Gennari, Laura Mecozzi, Vito Pagliarulo, Martina Mugnano, Emilia Oleandro, Filomena Nazzaro, Pietro Ferraro, Simonetta Grilli
Veröffentlicht in: Frontiers in Chemistry, Ausgabe 7, 2019, ISSN 2296-2646
Herausgeber: Frontiers
DOI: 10.3389/fchem.2019.00429

A pin-based pyro-electrohydrodynamic jet sensor for tuning theaccumulation of biomolecules down to sub-picogram level detection

Autoren: The detection of low abundant biomarkers is of great interest in different clinical cases, such as the early diagnosis of Alzheimer's disease. Here we present what we call pin-based pyro-electrohydrodynamic jet (p-jet) sensor that can linearly tune the accumulation rate of biomolecules in tiny droplets. In this newly revealed pinbased configuration we demonstrate the ability to detect biomolecules
Veröffentlicht in: Sensing and Bio-Sensing Research, Ausgabe 22141804, 2022, ISSN 2214-1804
Herausgeber: Elsevier BV
DOI: 10.1016/j.sbsr.2022.100536

Thesis and dissertations (2)

Dispensing pico droplets by pyroelectrohydrodynamic jetting: simulations and experimental results

Autoren: Anna Faraone
Veröffentlicht in: 2019
Herausgeber: Zendo
DOI: 10.5281/zenodo.3530462

Numerical simulations of droplet dynamics through an orifice – pin system for pyroelectrodynamic shooting

Autoren: Pentella Domenico
Veröffentlicht in: 2020
Herausgeber: Zenodo
DOI: 10.5281/zenodo.4767244

Conference proceedings (2)

SensApp: a FET-open project for developing a supersensor able to detect Alzheimer's disease biomarkers in blood

Autoren: Romina Rega, Martina Mugnano, Danila del Giudice, Simona Itri, Volodymyr Tkachenko, Veronica Vespini, Sara Coppola, Pietro Ferraro, Heidi Ottevaere, Yunfeng Nie, Sanna Uusitalo, Reinhard Schwoediauer, Martin Kaltenbrunner, Markku Känsäkoski, Emanuela Mazzon, Pier Luca Maffettone, Gaetano D'Avino, Simonetta Grilli
Veröffentlicht in: Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 2021, Seite(n) 58, ISBN 9781510644076
Herausgeber: SPIE
DOI: 10.1117/12.2596966

Highly sensitive detection of low abundant molecules by pyro-electrohydro-dynamic jetting

Autoren: ROMINA REGA, Martina Mugnano, Danila Del Giudice, Simona Itri, Volodymyr Tkachenko, veronica Vespini, Sara Coppola, Emanuela Mazzon, Emilia Oleandro, Pietro Ferraro, Simonetta Grilli
Veröffentlicht in: Biophotonics in Point-of-Care, 2020, Seite(n) 38, ISBN 9781510634954
Herausgeber: SPIE
DOI: 10.1117/12.2564859