A DNA NANOtechology toolkit for artificial CELL design

Información del proyecto

NANOCELL

Identificador del acuerdo de subvención: 851667

DOI

10.3030/851667

Proyecto cerrado

Fecha de la firma de la CE 4 Octubre 2019

Fecha de inicio 1 Enero 2020

Fecha de finalización 30 Septiembre 2025

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 1 497 477,00

Aportación de la UE

€ 1 497 477,00

Coordinado por

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
United Kingdom

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Publicaciones

Peer reviewed articles (29)

Amphiphilic DNA nanostructures for bottom-up synthetic biology.

Autores: Roger Rubio-Sánchez; Roger Rubio-Sánchez; Giacomo Fabrini; Pietro Cicuta; Lorenzo Di Michele; Lorenzo Di Michele
Publicado en: ChemComm, 2021, ISSN 1359-7345
Editor: Royal Society of Chemistry
DOI: 10.1039/d1cc04311k

Cation-responsive and photocleavable hydrogels from non-canonical amphiphilic DNA nanostructures

Autores: Giacomo Fabrini; Aisling Minard; Ryan A. Brady; Marco Di Antonio; Lorenzo Di Michele
Publicado en: Nano Letters, 2022, ISSN 1530-6984
Editor: American Chemical Society
DOI: 10.1021/acs.nanolett.1c03314

Detecting Nanoscale Distribution of Protein Pairs by Proximity-Dependent Super-resolution Microscopy.

Autores: Alexander H. Clowsley; William T. Kaufhold; Tobias Lutz; Anna Meletiou; Lorenzo Di Michele; Christian Soeller
Publicado en: Journal of the American Chemical Society, 2020, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1101/591081

Interplay of the mechanical and structural properties of DNA nanostructures determines their electrostatic interactions with lipid membranes

Autores: Diana Morzy; Cem Tekin; Vincenzo Caroprese; Roger Rubio-Sánchez; Lorenzo Di Michele; Maartje M. C. Bastings
Publicado en: Nanoscale, 2023, ISSN 2040-3372
Editor: Royal Society of Chemistry
DOI: 10.1039/d2nr05368c

Thermally Driven Membrane Phase Transitions Enable Content Reshuffling in Primitive Cells.

Autores: Roger Rubio-Sánchez; Derek K. O’Flaherty; Anna Wang; Francesca Coscia; Gianluca Petris; Lorenzo Di Michele; Lorenzo Di Michele; Pietro Cicuta; Claudia Bonfio; Claudia Bonfio
Publicado en: Journal of the American Chemical Society, Edición 5, 2021, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.1c06595

Haptotactic Motion of Multivalent Vesicles Along Ligand-Density Gradients

Autores: Hannah Sleath, Bortolo M. Mognetti, Yuval Elani, Lorenzo Di Michele
Publicado en: Langmuir, Edición 41, 2025, Página(s) 11474-11485, ISSN 0743-7463
Editor: American Chemical Society
DOI: 10.1021/acs.langmuir.5c00494

Modular RNA motifs for orthogonal phase separated compartments

Autores: Jaimie Marie Stewart, Shiyi Li, Anli Tang, Melissa Ann Klocke, Martin Vincent Gobry, Giacomo Fabrini, Lorenzo Di Michele, Paul W.K. Rothemund, Elisa Franco
Publicado en: Nature Communications, 2025, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1101/2023.10.06.561123

Thermal oscillations enable reshuffling of genetic material in a primitive cell cycle

Autores: Roger Rubio-Sánchez, Derek O’Flaherty, Anna Wang, Francesca Coscia, Lorenzo Di Michele, Pietro Cicuta, Claudia Bonfio
Publicado en: Journal of the American Chemical Society, 2024, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1101/2021.04.01.438038

Internal Phase Separation in Synthetic DNA Condensates

Autores: Diana A. Tanase, Dino Osmanović, Roger Rubio‐Sánchez, Layla Malouf, Elisa Franco, Lorenzo Di Michele
Publicado en: Advanced Science, Edición 12, 2025, ISSN 2198-3844
Editor: Wiley
DOI: 10.1002/advs.202506275

Cations Regulate Membrane Attachment and Functionality of DNA Nanostructures

Autores: Diana Morzy, Roger Rubio-Sánchez, Himanshu Joshi, Aleksei Aksimentiev, Lorenzo Di Michele, Ulrich F. Keyser
Publicado en: Journal of the American Chemical Society, Edición 143/19, 2021, Página(s) 7358-7367, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.1c00166

Co-transcriptional production of programmable RNA condensates and synthetic organelles

Autores: Giacomo Fabrini, Nada Farag, Sabrina Pia Nuccio, Shiyi Li, Jaimie M. Stewart, Anli A. Tang, Reece McCoy, Róisín M. Owens, Paul W. K. Rothemund, Elisa Franco, Marco Di Antonio, Lorenzo Di Michele
Publicado en: Nature Nanotechnology, 2025, ISSN 1748-3387
Editor: Nature Publishing Group
DOI: 10.1101/2023.10.06.561174

The ALS/FTD-related C9orf72 hexanucleotide repeat expansion forms RNA condensates through multimolecular G-quadruplexes

Autores: Federica Raguseo; Yiran Wang; Jessica Li; Marija Petrić Howe; Rubika Balendra; Anouk Huyghebaert; Devkee M. Vadukul; Diana A. Tanase; Thomas E. Maher; Layla Malouf; Roger Rubio-Sánchez; Francesco A. Aprile; Yuval Elani; Rickie Patani; Lorenzo Di Michele; Marco Di Antonio
Publicado en: Nature Communications, 2023, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-023-43872-1

Fast, multicolour optical sectioning over extended fields of view with patterned illumination and machine learning

Autores: Ward, Edward N; McClelland, Rebecca M; Lamb, Jacob R; Rubio-Sánchez, Roger; Christensen, Charles N; Mazumder, Bismoy; Kapsiani, Sofia; Mascheroni, Luca; Di Michele, Lorenzo; Kaminski Schierle, Gabriele S; Kaminski, Clemens F
Publicado en: Biomedical Optics Express, Edición 3, 2024, ISSN 2156-7085
Editor: The Optical Society
DOI: 10.1364/boe.510912

Modulating membrane fusion through the design of fusogenic DNA circuits and bilayer composition

Autores: Miguel Paez-Perez; I. Alasdair Russell; Pietro Cicuta; Lorenzo Di Michele
Publicado en: Soft Matter, 2022, ISSN 1744-683X
Editor: Royal Society of Chemistry
DOI: 10.1039/d2sm00863g

Probing the Mechanical Properties of DNA Nanostructures with Metadynamics

Autores: Will T. Kaufhold, Wolfgang Pfeifer, Carlos E. Castro, and Lorenzo Di Michele
Publicado en: ACS Nano, 2022, ISSN 1936-0851
Editor: American Chemical Society
DOI: 10.1021/acsnano.1c08999

Patterning and dynamics of membrane adhesion under hydraulic stress

Autores: Céline Dinet, Alejandro Torres-Sánchez, Roberta Lanfranco, Lorenzo Di Michele, Marino Arroyo, Margarita Staykova
Publicado en: Nature Communications, Edición 14, 2023, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-023-43246-7

Sculpting DNA-based synthetic cells through phase separation and phase-targeted activity

Autores: Layla Malouf; Diana A. Tanase; Giacomo Fabrini; Miguel Paez-Perez; Adrian Leathers; Michael J. Booth; Lorenzo Di Michele
Publicado en: Chem, 2023, ISSN 2451-9294
Editor: Cell Press
DOI: 10.1016/j.chempr.2023.10.004

Adaptable DNA interactions regulate surface triggered self assembly

Autores: Roberta Lanfranco, Pritam Kumar Jana, Gilles Bruylants, Pietro Cicuta, Bortolo Matteo Mognetti, Lorenzo Di Michele
Publicado en: Nanoscale, Edición 12/36, 2020, Página(s) 18616-18620, ISSN 2040-3364
Editor: Royal Society of Chemistry
DOI: 10.1039/d0nr04461j

Reaction-diffusion patterning of DNA-based artificial cells

Autores: Adrian Leathers; Michal Walczak; Ryan A. Brady; Assala Al Samad; Jurij Kotar; Michael J. Booth; Pietro Cicuta; Lorenzo Di Michele
Publicado en: Journal of the American Chemical Society, 2022, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.2c06140

DNA-Based Optical Quantification of Ion Transport across Giant Vesicles

Autores: Marcus Fletcher; Jinbo Zhu; Roger Rubio-Sánchez; Sarah E Sandler; Kareem Al Nahas; Lorenzo Di Michele; Ulrich F Keyser; Ran Tivony
Publicado en: ACS Nano, 2023, ISSN 1936-0851
Editor: American Chemical Society
DOI: 10.1021/acsnano.2c07496

DNA-Origami Line-Actants Control Domain Organization and Fission in Synthetic Membranes

Autores: Roger Rubio-Sánchez; Bortolo Matteo Mognetti; Pietro Cicuta; Lorenzo Di Michele
Publicado en: Journal of the American Chemical Society, 2022, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.3c01493

A Modular, Dynamic, DNA-Based Platform for Regulating Cargo Distribution and Transport between Lipid Domains

Autores: Roger Rubio-Sánchez, Simone Eizagirre Barker, Michal Walczak, Pietro Cicuta, Lorenzo Di Michele
Publicado en: Nano Letters, Edición 21/7, 2021, Página(s) 2800-2808, ISSN 1530-6984
Editor: American Chemical Society
DOI: 10.1021/acs.nanolett.0c04867

A Synthetic Signaling Network Imitating the Actionof Immune Cells in Response to Bacterial Metabolism

Autores: Michal Walczak, Leonardo Mancini, Jiayi Xu, Federica Raguseo, Jurij Kotar, Pietro Cicuta,and Lorenzo Di Michele
Publicado en: Advanced Materials, 2023, ISSN 1521-4095
Editor: Wiley
DOI: 10.1002/adma.202301562

Lipid vesicle-based molecular robots

Autores: Zugui Peng; Shoji Iwabuchi; Kayano Izumi; Sotaro Takiguchi; Misa Yamaji; Shoko Fujita; Harune Suzuki; Fumika Kambara; Genki Fukasawa; Aileen Cooney; Lorenzo Di Michele; Yuval Elani; Tomoaki Matsuura; Ryuji Kawano
Publicado en: Lab on a Chip, 2024, ISSN 1473-0189
Editor: Royal Society of Chemistry
DOI: 10.1039/d3lc00860f

Influence of hydrophobic moieties on the crystallization of amphiphilic DNA nanostructures

Autores: Jurij Kotar; Adrian Leathers; Michal Walczak; Ryan Brady; Lorenzo Di Michele
Publicado en: The Journal of Chemical Physics, 2023, ISSN 0021-9606
Editor: American Institute of Physics
DOI: 10.1063/5.0132484

Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy

Autores: Alexander H. Clowsley, William T. Kaufhold, Tobias Lutz, Anna Meletiou, Lorenzo Di Michele, Christian Soeller
Publicado en: Nature Communications, Edición 12/1, 2021, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-020-20686-z

DNA-assisted selective electrofusion (DASE) of <i>Escherichia coli</i> and giant lipid vesicles

Autores: Pietro Cicuta; Lorenzo Di Michele; Sho Takamori; Shoji Takeuchi
Publicado en: Nanoscale, 2022, ISSN 2040-3372
Editor: Royal Society of Chemistry
DOI: 10.1039/d2nr03105a

Cation-Controlled Assembly, Activity, and Organization of Biomimetic DNA Receptors in Synthetic Cell Membranes

Autores: Elita Peters, Diana A. Tanase, Lorenzo Di Michele, Roger Rubio-Sánchez
Publicado en: Journal of the American Chemical Society, Edición 147, 2025, Página(s) 33780-33789, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.5c09928

Responsive core-shell DNA particles trigger lipid-membrane disruption and bacteria entrapment

Autores: Michal Walczak, Ryan A. Brady, Leonardo Mancini, Claudia Contini, Roger Rubio-Sánchez, William T. Kaufhold, Pietro Cicuta, Lorenzo Di Michele
Publicado en: Nature Communications, Edición 12/1, 2021, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-021-24989-7

Non-peer reviewed articles (1)

Enzyme-Responsive DNA Condensates

Autores: Juliette Bucci, Layla Malouf, Diana A. Tanase, Nada Farag, Jacob R. Lamb, Roger Rubio-Sánchez, Serena Gentile, Erica Del Grosso, Clemens F. Kaminski, Lorenzo Di Michele, Francesco Ricci
Publicado en: Journal of the American Chemical Society, Edición 146, 2024, Página(s) 31529-31537, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.4c08919