Descripción del proyecto
Hacer zoom en las estructuras proteicas podría ampliar significativamente las oportunidades de tratamiento de la disfunción
Las proteínas, enzimas y hormonas son moléculas grandes y complejas necesarias para la mayoría de las funciones celulares. Como es de esperar, la disfunción proteica es la raíz de numerosas enfermedades y afecciones. Un mecanismo característico de la actividad proteica es agregarse a otras moléculas en sitios específicos mediante cambios conformacionales. La caracterización precisa de la estructura 3D detallada subyacente a funciones específicas es fundamental para avanzar en la comprensión. La criomicroscopía electrónica ganadora de un premio Nobel permite la toma de imágenes de especímenes hidratados congelados en su estado nativo sin tintes ni fijadores y ha abierto una nueva ventana a las estructuras celulares finas y la resolución molecular. IMpaCT está utilizando esta técnica revolucionaria para visualizar proteínas relacionadas con la salud humana, a la vez que aumenta la visibilidad de la universidad coordinadora.
Objetivo
Proteins are building blocks of life, often forming complexes with essential cellular roles. Dysfunction of proteins or their complexes in cells are associated with a wide variety of pathologies, making them excellent drug target candidates. Their 3D structures are essential for a rational drug design. However, many important proteins and complexes are extremely hard to crystallize. Recent advances in Cryo-Electron Microscopy methodologies enabled the structure determination of large macromolecular complexes at near-atomic resolution. An unprecedented level of detail in cell imaging using Cryo-Electron Tomography is also now possible. IMpaCT will improve the expertise at the UNL in Cryo-EM methodologies applied to the structural studies of proteins related to human health (e.g. cancer, antiviral infections) from individual molecules to macromolecular complexes, both isolated and in a cellular context. To this end, UNL will team up with renowned Cryo-EM experts from the National Centre for Biotechnology of the Spanish National Research Council (Spain), the Institute of Biotechnology of the University of Helsinki (Finland) and the Weizmann Institute of Science (Israel). A solid work programme grounded on hands-on Workshops will be complemented with short-term visits to the partner labs, visits from partner labs experts to ITQB NOVA, seminars by leading experts in Cryo-EM (e.g. Nobel Prize winners), industry stakeholders’ engagement via a Mini-symposium and workshop participation, knowledge transfer to other research groups in Portugal and a WP devoted to early-stage researchers. An ambitious Dissemination, Exploitation and Communication plan will be implemented to leverage the research results potentiated by this project as high-impact publications and invited communications at international meetings, leading to a fundamental outcome: an increased visibility and activity of UNL researchers as players in the Cryo-EM field.
Ámbito científico
Palabras clave
Programa(s)
- H2020-EU.4.b. - Twinning of research institutions Main Programme
Tema(s)
Convocatoria de propuestas
Consulte otros proyectos de esta convocatoriaConvocatoria de subcontratación
H2020-WIDESPREAD-2018-03
Régimen de financiación
CSA - Coordination and support actionCoordinador
1099 085 Lisboa
Portugal