DNA helicases in genome maintenance: from molecular and cellular mechanisms to specific inhibitors as potential drugs

Project Information

AntiHelix

Grant agreement ID: 859853

DOI

10.3030/859853

Project closed

EC signature date 9 August 2019

Start date 1 October 2019

End date 30 September 2024

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost

€ 3 508 622,28

EU contribution

€ 3 508 622,28

Coordinated by

CONSIGLIO NAZIONALE DELLE RICERCHE
Italy

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Other (8)

Training Events M25-M48

"The following Training Events have been planned:1. NAPLES, Italy (M28): Transferable Skills Course #3 (2 day); Hands-on Practical Class #3 (2 days).2. AMSTERDAM, Netherlands (M34): Research Theoretical Course #3 (2 days); Transferable Skills Course #4 (1 day); Hands-on Practical Class #4 (2 days).3. ESSEN; Germany (M42): Research Theoretical Course #4 (1 day); Transferable Skills Course #5 (1 day); Hands-on Practical Class #5 (3 days)."

List of publications in science communication magazines and/or national newspapers
Recruitment of ESRs
Award of PhD degrees for 3-year PhD courses
Training Events M1-M24

The following Training Events have been planned1TRIESTE Italy M13 Research Theoretical Course 1 1 day Transferable Skills Course 1 2 days Handson Practical Class 1 3 days2 DORTMUND Germany M20 Research Theoretical Course 2 2 days Transferable Skills Course 2 2 days Handson Practical Class 2 2 days

List of Workshops/Open Days
List of events connected with ESOF2020 and the European Researchers' Nights/Science Fairs

List of events connected with ESOF2020 and the European Researchers Nights/Science Fairs

AntiHelix Press Office

An international Press Office dedicated to the AntiHelix programme will be operated by SISSA Medialab (Trieste, Italy). Press releases will be periodically prepared by this Press Office for two main news services (Eurekalert and Alpha Galileo) to enhance the visibility and coverage of the project.

Documents, reports (2)

List of Joint Supervisors and local academic Supervisors
List of presentations at conferences by ESRs and PIs

Websites, patent fillings, videos etc. (3)

List of articles/videos by ESRs on web sites

A list of articles/videos by ESRs on web sites is planned at M

AntiHelix accounts on web social media
AntiHelix project website

Publications

Peer reviewed articles (25)

Ion-mediated condensation controls the mechanics of mitotic chromosomes

Author(s): Hannes Witt, Janni Harju, Emma M. J. Chameau, Charlotte M. A. Bruinsma, Tinka V. M. Clement, Christian F. Nielsen, Ian D. Hickson, Erwin J. G. Peterman, Chase P. Broedersz, Gijs J. L. Wuite
Published in: Nature Materials, Issue 23, 2024, Page(s) 1556-1562, ISSN 1476-1122
Publisher: Nature Publishing Group
DOI: 10.1038/s41563-024-01975-0

SCAI promotes error‐free repair of DNA interstrand crosslinks via the Fanconi anemia pathway

Author(s): Lisa Schubert, Ivo A Hendriks, Emil P T Hertz, Wei Wu, Selene Sellés‐Baiget, Saskia Hoffmann, Keerthana Stine Viswalingam, Irene Gallina, Satyakrishna Pentakota, Bente Benedict, Joachim Johansen, Katja Apelt, Martijn S Luijsterburg, Simon Rasmussen, Michael Lisby, Ying Liu, Michael L Nielsen, Niels Mailand, Julien P Duxin
Published in: EMBO reports, Issue 23, 2023, ISSN 1469-221X
Publisher: Nature Publishing Group
DOI: 10.15252/embr.202153639

RECQL4 affects MHC class II‐mediated signalling and favours an immune‐evasive signature that limits response to immune checkpoint inhibitor therapy in patients with malignant melanoma

Author(s): Sara Egea‐Rodriguez, Renáta Váraljai, Thierry M. Nordmann, Restuan Lubis, Manuel Philip, Florian Rambow, Alexander Roesch, Michael Flaig, Susanne Horn, Raphael Stoll, Fang Zhao, Annette Paschen, Bert Klebl, Ian D. Hickson, Dirk Schadendorf, Matthias Mann, Iris Helfrich
Published in: Clinical and Translational Medicine, Issue 15, 2025, ISSN 2001-1326
Publisher: Wiley online library
DOI: 10.1002/ctm2.70094

Optical tweezers for drug discovery

Author(s): Matthew T.J. Halma, Jack A. Tuszynski, Gijs J.L. Wuite
Published in: Drug Discovery Today, Issue 28, 2023, Page(s) 103443, ISSN 1359-6446
Publisher: Elsevier BV
DOI: 10.1016/j.drudis.2022.103443

Structural and biochemical characterization of the C‐terminal region of the human <scp>RTEL</scp>1 helicase

Author(s): Giuseppe Cortone, Melissa A. Graewert, Manil Kanade, Antonio Longo, Raghurama Hegde, Amaia González‐Magaña, Belén Chaves‐Arquero, Francisco J. Blanco, Luisa M. R. Napolitano, Silvia Onesti
Published in: Protein Science, Issue 33, 2024, ISSN 0961-8368
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.5093

Unravelling How Single-Stranded DNA Binding Protein Coordinates DNA Metabolism Using Single-Molecule Approaches

Author(s): Longfu Xu, Matthew T. J. Halma, Gijs J. L. Wuite
Published in: International Journal of Molecular Sciences, Issue 24, 2023, Page(s) 2806, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24032806

Nonlinear mechanics of human mitotic chromosomes

Author(s): Anna E. C. Meijering, Kata Sarlós, Christian F. Nielsen, Hannes Witt, Janni Harju, Emma Kerklingh, Guus H. Haasnoot, Anna H. Bizard, Iddo Heller, Chase P. Broedersz, Ying Liu, Erwin J. G. Peterman, Ian D. Hickson, Gijs J. L. Wuite
Published in: Nature, Issue 605, 2023, Page(s) 545-550, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-022-04666-5

Genomic integrity and mitochondrial metabolism defects in Warsaw syndrome cells: a comparison with Fanconi anemia

Author(s): Bottega R, Ravera S, Napolitano LMR, Chiappetta V, Zini N, Crescenzi B, Arniani S, Faleschini M, Cortone G, Faletra F, Medagli B, Sirchia F, Moretti M, de Lange J, Cappelli E, Mecucci C, Onesti S, Pisani FM, Savoia A.
Published in: Journal of cellular physiology, 2021, ISSN 0021-9541
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/jcp.30265

Folate stress induces SLX1- and RAD51-dependent mitotic DNA synthesis at the fragile X locus in human cells.

Author(s): Garribba L, Bjerregaard VA, Gonçalves Dinis MM, Özer Ö, Wu W, Sakellariou D, Pena-Diaz J, Hickson ID, Liu Y.
Published in: Proceedings of the National Academy of Sciences of the United States of America, 2020, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1921219117

Mapping fast DNA polymerase exchange during replication

Author(s): Longfu Xu, Matthew T. J. Halma, Gijs J. L. Wuite
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-49612-3

Therapeutic potential of compounds targeting SARS-CoV-2 helicase

Author(s): Matthew T. J. Halma, Mark J. A. Wever, Sanne Abeln, Didier Roche, Gijs J. L. Wuite
Published in: Frontiers in Chemistry, Issue 10, 2022, ISSN 2296-2646
Publisher: Frontiers Media SA
DOI: 10.3389/fchem.2022.1062352

Mitotic DNA synthesis in response to replication stress requires the sequential action of DNA polymerases zeta and delta in human cells

Author(s): Wei Wu, Szymon A. Barwacz, Rahul Bhowmick, Katrine Lundgaard, Marisa M. Gonçalves Dinis, Malgorzata Clausen, Masato T. Kanemaki, Ying Liu
Published in: Nature Communications, Issue 14, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-35992-5

FANCJ DNA helicase is recruited to the replisome by AND-1 to ensure genome stability

Author(s): Ana Boavida, Luisa MR Napolitano, Diana Santos, Giuseppe Cortone, Nanda K Jegadesan, Silvia Onesti, Dana Branzei, Francesca M Pisani
Published in: EMBO Reports, Issue 25, 2024, Page(s) 876-901, ISSN 1469-3178
Publisher: Springer Nature Group
DOI: 10.1038/s44319-023-00044-y

Spotlight on Warsaw Breakage Syndrome.

Author(s): Francesca M Pisani
Published in: The Application of Clinical Genetics, Vol Volume 12, Pp 239-248 (2019), Issue 2, 2019, ISSN 1178-704X
Publisher: Dove Medical Press Ltd
DOI: 10.2147/tacg.s186476

Functional Coupling between DNA Replication and Sister Chromatid Cohesion Establishment

Author(s): Ana Boavida; Diana Santos; Mohammad Mahtab; Francesca M. Pisani
Published in: International Journal of Molecular Sciences, Vol 22, Iss 2810, p 2810 (2021), Issue 1, 2021, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22062810

Regulation of T7 gp2.5 binding dynamics by its C-terminal tail, template conformation and sequence

Author(s): Longfu Xu, Jordi Cabanas-Danés, Matthew T J Halma, Iddo Heller, Sarah A Stratmann, Antoine M van Oijen, Seung-Joo Lee, Erwin J G Peterman, Gijs J L Wuite
Published in: Nucleic Acids Research, Issue 51, 2023, Page(s) 6540-6553, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkad485

Warsaw Breakage Syndrome associated DDX11 helicase resolves G-quadruplex structures to support sister chromatid cohesion

Author(s): van Schie JJM, Faramarz A, Balk JA, Stewart GS, Cantelli E, Oostra AB, Rooimans MA, Parish JL, de Almeida Estéves C, Dumic K, Barisic I, Diderich KEM, van Slegtenhorst MA, Mahtab M, Pisani FM, Te Riele H, Ameziane N, Wolthuis RMF, de Lange J.
Published in: Nature Communications, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-18066-8

The prevention and resolution of DNA replication–transcription conflicts in eukaryotic cells

Author(s): Wei Wu; Ian D. Hickson; Ying Liu
Published in: Genome Instability & Disease, Issue 1, 2020, ISSN 2524-7662
Publisher: Springer
DOI: 10.1007/s42764-020-00012-z

Structure-based discovery of first inhibitors targeting the helicase activity of human PIF1

Author(s): Mark J A Wever, Francesca R Scommegna, Sara Egea-Rodriguez, Saba Dehghani-Tafti, Jose Brandao-Neto, Jean-François Poisson, Iris Helfrich, Alfred A Antson, Vincent Rodeschini, Ben Bax, Didier Roche, Cyril M Sanders
Published in: Nucleic Acids Research, Issue 51, 2024, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkae897

Exploring the G-quadruplex binding and unwinding activity of the bacterial FeS helicase DinG

Author(s): Elisa De Piante, Federica D’Aria, Luisa M. R. Napolitano, Jussara Amato, Simone Pirrello, Silvia Onesti, Concetta Giancola
Published in: Scientific Reports, Issue 13, 2023, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-023-39675-5

RTEL1 suppresses G-quadruplex-associated R-loops at difficult-to-replicate loci in the human genome

Author(s): Wu W, Bhowmick R, Vogel I, Özer Ö, Ghisays F, Thakur RS, Sanchez de Leon E, Richter PH, Ren L, Petrini JH, Hickson ID, Liu Y
Published in: Nature structural and molecular biology, 2020, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/s41594-020-0408-6

Role of the DDX11 DNA Helicase in Warsaw Breakage Syndrome Etiology

Author(s): Ana Boavida; Francesca M. Pisani; Mohammad Mahtab; Diana Santos
Published in: International Journal of Molecular Sciences, Issue 1, 2021, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22052308

Completing genome replication outside of S phase

Author(s): Rahul Bhowmick, Ian D. Hickson, Ying Liu
Published in: Molecular Cell, Issue 83, 2024, Page(s) 3596-3607, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2023.08.023

The Genome Stability Maintenance DNA Helicase DDX11 and Its Role in Cancer

Author(s): Mohammad Mahtab; Ana Boavida; Diana Santos; Francesca M. Pisani
Published in: Genes, Issue 2, 2021, ISSN 2073-4425
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes12030395

Folate Deficiency Triggers the Abnormal Segregation of a Region With Large Cluster of CG-Rich Trinucleotide Repeats on Human Chromosome 2

Author(s): Garribba L, Vogel I, Lerdrup M, Gonçalves Dinis MM, Ren L, Liu Y
Published in: Frontiers in genetics, 2021, ISSN 1664-8021
Publisher: Frontiers Media
DOI: 10.3389/fgene.2021.695124

Conference proceedings (1)

P12.11 RECQL4 promotes immune evasion and limits response to anti-PD-1 therapy and survival in melanoma patients

Author(s): S Egea-Rodriguez, R Váraljai, TM Nordmann, R Lubis, M Philip, F Rambow, A Roesch, D Schadendorf, B Klebl, ID Hickson, M Mann, S Horn, I Helfrich
Published in: Poster Presentations, 2024, Page(s) A36.2-A37
Publisher: BMJ Publishing Group Ltd
DOI: 10.1136/jitc-2024-itoc10.65

Non-peer reviewed articles (1)

49th Annual Meeting of the Arbeitsgemeinschaft Dermatologische Forschung (ADF)

Author(s): Egea-Rodriguez, S., Philip, M., Váraljai, R., Schadendorf, D., Horn, S., & Helfrich, I.
Published in: Experimental Dermatology, Issue 32, 2023, ISSN 0906-6705
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/exd.14726