DNA helicases in genome maintenance: from molecular and cellular mechanisms to specific inhibitors as potential drugs

Informazioni relative al progetto

AntiHelix

ID dell’accordo di sovvenzione: 859853

DOI

10.3030/859853

Progetto chiuso

Data della firma CE 9 Agosto 2019

Data di avvio 1 Ottobre 2019

Data di completamento 30 Settembre 2024

Finanziato da

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Costo totale

€ 3 508 622,28

Contributo UE

€ 3 508 622,28

Coordinato da

CONSIGLIO NAZIONALE DELLE RICERCHE
Italy

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Other (8)

Training Events M25-M48

"The following Training Events have been planned:1. NAPLES, Italy (M28): Transferable Skills Course #3 (2 day); Hands-on Practical Class #3 (2 days).2. AMSTERDAM, Netherlands (M34): Research Theoretical Course #3 (2 days); Transferable Skills Course #4 (1 day); Hands-on Practical Class #4 (2 days).3. ESSEN; Germany (M42): Research Theoretical Course #4 (1 day); Transferable Skills Course #5 (1 day); Hands-on Practical Class #5 (3 days)."

List of publications in science communication magazines and/or national newspapers
Recruitment of ESRs
Award of PhD degrees for 3-year PhD courses
Training Events M1-M24

The following Training Events have been planned1TRIESTE Italy M13 Research Theoretical Course 1 1 day Transferable Skills Course 1 2 days Handson Practical Class 1 3 days2 DORTMUND Germany M20 Research Theoretical Course 2 2 days Transferable Skills Course 2 2 days Handson Practical Class 2 2 days

List of Workshops/Open Days
List of events connected with ESOF2020 and the European Researchers' Nights/Science Fairs

List of events connected with ESOF2020 and the European Researchers Nights/Science Fairs

AntiHelix Press Office

An international Press Office dedicated to the AntiHelix programme will be operated by SISSA Medialab (Trieste, Italy). Press releases will be periodically prepared by this Press Office for two main news services (Eurekalert and Alpha Galileo) to enhance the visibility and coverage of the project.

Documents, reports (2)

List of Joint Supervisors and local academic Supervisors
List of presentations at conferences by ESRs and PIs

Websites, patent fillings, videos etc. (3)

List of articles/videos by ESRs on web sites

A list of articles/videos by ESRs on web sites is planned at M

AntiHelix accounts on web social media
AntiHelix project website

Pubblicazioni

Peer reviewed articles (25)

Ion-mediated condensation controls the mechanics of mitotic chromosomes

Autori: Hannes Witt, Janni Harju, Emma M. J. Chameau, Charlotte M. A. Bruinsma, Tinka V. M. Clement, Christian F. Nielsen, Ian D. Hickson, Erwin J. G. Peterman, Chase P. Broedersz, Gijs J. L. Wuite
Pubblicato in: Nature Materials, Numero 23, 2024, Pagina/e 1556-1562, ISSN 1476-1122
Editore: Nature Publishing Group
DOI: 10.1038/s41563-024-01975-0

SCAI promotes error‐free repair of DNA interstrand crosslinks via the Fanconi anemia pathway

Autori: Lisa Schubert, Ivo A Hendriks, Emil P T Hertz, Wei Wu, Selene Sellés‐Baiget, Saskia Hoffmann, Keerthana Stine Viswalingam, Irene Gallina, Satyakrishna Pentakota, Bente Benedict, Joachim Johansen, Katja Apelt, Martijn S Luijsterburg, Simon Rasmussen, Michael Lisby, Ying Liu, Michael L Nielsen, Niels Mailand, Julien P Duxin
Pubblicato in: EMBO reports, Numero 23, 2023, ISSN 1469-221X
Editore: Nature Publishing Group
DOI: 10.15252/embr.202153639

RECQL4 affects MHC class II‐mediated signalling and favours an immune‐evasive signature that limits response to immune checkpoint inhibitor therapy in patients with malignant melanoma

Autori: Sara Egea‐Rodriguez, Renáta Váraljai, Thierry M. Nordmann, Restuan Lubis, Manuel Philip, Florian Rambow, Alexander Roesch, Michael Flaig, Susanne Horn, Raphael Stoll, Fang Zhao, Annette Paschen, Bert Klebl, Ian D. Hickson, Dirk Schadendorf, Matthias Mann, Iris Helfrich
Pubblicato in: Clinical and Translational Medicine, Numero 15, 2025, ISSN 2001-1326
Editore: Wiley online library
DOI: 10.1002/ctm2.70094

Optical tweezers for drug discovery

Autori: Matthew T.J. Halma, Jack A. Tuszynski, Gijs J.L. Wuite
Pubblicato in: Drug Discovery Today, Numero 28, 2023, Pagina/e 103443, ISSN 1359-6446
Editore: Elsevier BV
DOI: 10.1016/j.drudis.2022.103443

Structural and biochemical characterization of the C‐terminal region of the human <scp>RTEL</scp>1 helicase

Autori: Giuseppe Cortone, Melissa A. Graewert, Manil Kanade, Antonio Longo, Raghurama Hegde, Amaia González‐Magaña, Belén Chaves‐Arquero, Francisco J. Blanco, Luisa M. R. Napolitano, Silvia Onesti
Pubblicato in: Protein Science, Numero 33, 2024, ISSN 0961-8368
Editore: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.5093

Unravelling How Single-Stranded DNA Binding Protein Coordinates DNA Metabolism Using Single-Molecule Approaches

Autori: Longfu Xu, Matthew T. J. Halma, Gijs J. L. Wuite
Pubblicato in: International Journal of Molecular Sciences, Numero 24, 2023, Pagina/e 2806, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24032806

Nonlinear mechanics of human mitotic chromosomes

Autori: Anna E. C. Meijering, Kata Sarlós, Christian F. Nielsen, Hannes Witt, Janni Harju, Emma Kerklingh, Guus H. Haasnoot, Anna H. Bizard, Iddo Heller, Chase P. Broedersz, Ying Liu, Erwin J. G. Peterman, Ian D. Hickson, Gijs J. L. Wuite
Pubblicato in: Nature, Numero 605, 2023, Pagina/e 545-550, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/s41586-022-04666-5

Genomic integrity and mitochondrial metabolism defects in Warsaw syndrome cells: a comparison with Fanconi anemia

Autori: Bottega R, Ravera S, Napolitano LMR, Chiappetta V, Zini N, Crescenzi B, Arniani S, Faleschini M, Cortone G, Faletra F, Medagli B, Sirchia F, Moretti M, de Lange J, Cappelli E, Mecucci C, Onesti S, Pisani FM, Savoia A.
Pubblicato in: Journal of cellular physiology, 2021, ISSN 0021-9541
Editore: John Wiley & Sons Inc.
DOI: 10.1002/jcp.30265

Folate stress induces SLX1- and RAD51-dependent mitotic DNA synthesis at the fragile X locus in human cells.

Autori: Garribba L, Bjerregaard VA, Gonçalves Dinis MM, Özer Ö, Wu W, Sakellariou D, Pena-Diaz J, Hickson ID, Liu Y.
Pubblicato in: Proceedings of the National Academy of Sciences of the United States of America, 2020, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.1921219117

Mapping fast DNA polymerase exchange during replication

Autori: Longfu Xu, Matthew T. J. Halma, Gijs J. L. Wuite
Pubblicato in: Nature Communications, Numero 15, 2024, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-024-49612-3

Therapeutic potential of compounds targeting SARS-CoV-2 helicase

Autori: Matthew T. J. Halma, Mark J. A. Wever, Sanne Abeln, Didier Roche, Gijs J. L. Wuite
Pubblicato in: Frontiers in Chemistry, Numero 10, 2022, ISSN 2296-2646
Editore: Frontiers Media SA
DOI: 10.3389/fchem.2022.1062352

Mitotic DNA synthesis in response to replication stress requires the sequential action of DNA polymerases zeta and delta in human cells

Autori: Wei Wu, Szymon A. Barwacz, Rahul Bhowmick, Katrine Lundgaard, Marisa M. Gonçalves Dinis, Malgorzata Clausen, Masato T. Kanemaki, Ying Liu
Pubblicato in: Nature Communications, Numero 14, 2023, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-35992-5

FANCJ DNA helicase is recruited to the replisome by AND-1 to ensure genome stability

Autori: Ana Boavida, Luisa MR Napolitano, Diana Santos, Giuseppe Cortone, Nanda K Jegadesan, Silvia Onesti, Dana Branzei, Francesca M Pisani
Pubblicato in: EMBO Reports, Numero 25, 2024, Pagina/e 876-901, ISSN 1469-3178
Editore: Springer Nature Group
DOI: 10.1038/s44319-023-00044-y

Spotlight on Warsaw Breakage Syndrome.

Autori: Francesca M Pisani
Pubblicato in: The Application of Clinical Genetics, Vol Volume 12, Pp 239-248 (2019), Numero 2, 2019, ISSN 1178-704X
Editore: Dove Medical Press Ltd
DOI: 10.2147/tacg.s186476

Functional Coupling between DNA Replication and Sister Chromatid Cohesion Establishment

Autori: Ana Boavida; Diana Santos; Mohammad Mahtab; Francesca M. Pisani
Pubblicato in: International Journal of Molecular Sciences, Vol 22, Iss 2810, p 2810 (2021), Numero 1, 2021, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22062810

Regulation of T7 gp2.5 binding dynamics by its C-terminal tail, template conformation and sequence

Autori: Longfu Xu, Jordi Cabanas-Danés, Matthew T J Halma, Iddo Heller, Sarah A Stratmann, Antoine M van Oijen, Seung-Joo Lee, Erwin J G Peterman, Gijs J L Wuite
Pubblicato in: Nucleic Acids Research, Numero 51, 2023, Pagina/e 6540-6553, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkad485

Warsaw Breakage Syndrome associated DDX11 helicase resolves G-quadruplex structures to support sister chromatid cohesion

Autori: van Schie JJM, Faramarz A, Balk JA, Stewart GS, Cantelli E, Oostra AB, Rooimans MA, Parish JL, de Almeida Estéves C, Dumic K, Barisic I, Diderich KEM, van Slegtenhorst MA, Mahtab M, Pisani FM, Te Riele H, Ameziane N, Wolthuis RMF, de Lange J.
Pubblicato in: Nature Communications, 2020, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-020-18066-8

The prevention and resolution of DNA replication–transcription conflicts in eukaryotic cells

Autori: Wei Wu; Ian D. Hickson; Ying Liu
Pubblicato in: Genome Instability & Disease, Numero 1, 2020, ISSN 2524-7662
Editore: Springer
DOI: 10.1007/s42764-020-00012-z

Structure-based discovery of first inhibitors targeting the helicase activity of human PIF1

Autori: Mark J A Wever, Francesca R Scommegna, Sara Egea-Rodriguez, Saba Dehghani-Tafti, Jose Brandao-Neto, Jean-François Poisson, Iris Helfrich, Alfred A Antson, Vincent Rodeschini, Ben Bax, Didier Roche, Cyril M Sanders
Pubblicato in: Nucleic Acids Research, Numero 51, 2024, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkae897

Exploring the G-quadruplex binding and unwinding activity of the bacterial FeS helicase DinG

Autori: Elisa De Piante, Federica D’Aria, Luisa M. R. Napolitano, Jussara Amato, Simone Pirrello, Silvia Onesti, Concetta Giancola
Pubblicato in: Scientific Reports, Numero 13, 2023, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-023-39675-5

RTEL1 suppresses G-quadruplex-associated R-loops at difficult-to-replicate loci in the human genome

Autori: Wu W, Bhowmick R, Vogel I, Özer Ö, Ghisays F, Thakur RS, Sanchez de Leon E, Richter PH, Ren L, Petrini JH, Hickson ID, Liu Y
Pubblicato in: Nature structural and molecular biology, 2020, ISSN 1545-9993
Editore: Nature Publishing Group
DOI: 10.1038/s41594-020-0408-6

Role of the DDX11 DNA Helicase in Warsaw Breakage Syndrome Etiology

Autori: Ana Boavida; Francesca M. Pisani; Mohammad Mahtab; Diana Santos
Pubblicato in: International Journal of Molecular Sciences, Numero 1, 2021, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22052308

Completing genome replication outside of S phase

Autori: Rahul Bhowmick, Ian D. Hickson, Ying Liu
Pubblicato in: Molecular Cell, Numero 83, 2024, Pagina/e 3596-3607, ISSN 1097-2765
Editore: Cell Press
DOI: 10.1016/j.molcel.2023.08.023

The Genome Stability Maintenance DNA Helicase DDX11 and Its Role in Cancer

Autori: Mohammad Mahtab; Ana Boavida; Diana Santos; Francesca M. Pisani
Pubblicato in: Genes, Numero 2, 2021, ISSN 2073-4425
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes12030395

Folate Deficiency Triggers the Abnormal Segregation of a Region With Large Cluster of CG-Rich Trinucleotide Repeats on Human Chromosome 2

Autori: Garribba L, Vogel I, Lerdrup M, Gonçalves Dinis MM, Ren L, Liu Y
Pubblicato in: Frontiers in genetics, 2021, ISSN 1664-8021
Editore: Frontiers Media
DOI: 10.3389/fgene.2021.695124

Conference proceedings (1)

P12.11 RECQL4 promotes immune evasion and limits response to anti-PD-1 therapy and survival in melanoma patients

Autori: S Egea-Rodriguez, R Váraljai, TM Nordmann, R Lubis, M Philip, F Rambow, A Roesch, D Schadendorf, B Klebl, ID Hickson, M Mann, S Horn, I Helfrich
Pubblicato in: Poster Presentations, 2024, Pagina/e A36.2-A37
Editore: BMJ Publishing Group Ltd
DOI: 10.1136/jitc-2024-itoc10.65

Non-peer reviewed articles (1)

49th Annual Meeting of the Arbeitsgemeinschaft Dermatologische Forschung (ADF)

Autori: Egea-Rodriguez, S., Philip, M., Váraljai, R., Schadendorf, D., Horn, S., & Helfrich, I.
Pubblicato in: Experimental Dermatology, Numero 32, 2023, ISSN 0906-6705
Editore: Blackwell Publishing Inc.
DOI: 10.1111/exd.14726