Periodic Reporting for period 1 - PROTON (Proton transport and proton-coupled transport)
Okres sprawozdawczy: 2019-09-01 do 2021-08-31
Secure, competitive, and sustainable energy production is a major challenge facing human societies. Biomimetic solutions such as the development of new biofuel cells are hampered by our thus far incomplete understanding of proton transfer reactions. The same holds for health threats to humanity: Curing diseases like cancer, obesity, chronic gastritis, gastric and duodenal ulcers, requires to pharmacologically interfere - in their molecular details - with yet unresolved proton transfer reactions.
Here we aim at clarifying the molecular reaction mechanism in the confines of interfacial water layers and proteinaceous cavities with emphasis on arrangement and mobility of proton relay moieties. Achieving this requires an interdisciplinary, multi-level approach comprising cutting edge technologies like second harmonic imaging, single molecule and time resolved fluorescence microscopy and spectroscopy, advanced calculations of proton transfer, bioengineering of membrane channel and transporter containing systems, synthetic design of biomimetic proton channels, solving protein structures and rational drug design.
Proton transfer is crucial in numerous biological and chemical processes, e.g. in cellular proton pumps or in hydrogen fuel cells. Even though their empirical study began with the origin of chemistry, many details of the proton transfer mechanism are still unresolved and understanding the way in which confined water mediates proton dynamics remains a fundamental challenge in chemistry and biochemistry. Transmembrane proton gradients are essential to life on earth as they are intricately linked to both photosynthesis and synthesis of adenosine triphosphate (ATP, the energy currency of life). Yet, once protons have crossed the membrane, they do not freely exchange with protons on the receiving site. An energy barrier with the height of ~30 kT opposes their release into the bulk. The mainly entropic nature of the barrier ensures high lateral proton mobility. However, besides being attributed to structured water,,the molecular origin of that barrier remained thus far elusive. Yet, newly developed label-free and charge-sensitive dynamic imaging techniques of lipid membrane hydration, hydration of active protein sites as well as their dipolar relaxation dynamics now offer the possibility to explore the interplay between structural features of the hydration shell and proton migration on the millisecond time scale. Likewise, technically demanding ab-initio molecular dynamics (MD) simulations of protons adjacent to lipid bilayers also promise insight into the molecular proton migration mechanism. By levering on these new methods for (i) visualising proton surface transport as well as (ii) assessing its energetics and combining them with approaches for deciphering the structure of G-protein-coupled receptors (GPCRs) and other proton-dependent membrane protein, the PROTON project will perform ground-breaking work in this field.
Here we aim at clarifying the molecular reaction mechanism in the confines of interfacial water layers and proteinaceous cavities with emphasis on arrangement and mobility of proton relay moieties. Achieving this requires an interdisciplinary, multi-level approach comprising cutting edge technologies like second harmonic imaging, single molecule and time resolved fluorescence microscopy and spectroscopy, advanced calculations of proton transfer, bioengineering of membrane channel and transporter containing systems, synthetic design of biomimetic proton channels, solving protein structures and rational drug design.
Proton transfer is crucial in numerous biological and chemical processes, e.g. in cellular proton pumps or in hydrogen fuel cells. Even though their empirical study began with the origin of chemistry, many details of the proton transfer mechanism are still unresolved and understanding the way in which confined water mediates proton dynamics remains a fundamental challenge in chemistry and biochemistry. Transmembrane proton gradients are essential to life on earth as they are intricately linked to both photosynthesis and synthesis of adenosine triphosphate (ATP, the energy currency of life). Yet, once protons have crossed the membrane, they do not freely exchange with protons on the receiving site. An energy barrier with the height of ~30 kT opposes their release into the bulk. The mainly entropic nature of the barrier ensures high lateral proton mobility. However, besides being attributed to structured water,,the molecular origin of that barrier remained thus far elusive. Yet, newly developed label-free and charge-sensitive dynamic imaging techniques of lipid membrane hydration, hydration of active protein sites as well as their dipolar relaxation dynamics now offer the possibility to explore the interplay between structural features of the hydration shell and proton migration on the millisecond time scale. Likewise, technically demanding ab-initio molecular dynamics (MD) simulations of protons adjacent to lipid bilayers also promise insight into the molecular proton migration mechanism. By levering on these new methods for (i) visualising proton surface transport as well as (ii) assessing its energetics and combining them with approaches for deciphering the structure of G-protein-coupled receptors (GPCRs) and other proton-dependent membrane protein, the PROTON project will perform ground-breaking work in this field.
PROTON has recruited, enrolled in PhD programms and started training of 15 PhD students. Two training events where organised where PhD students, beneficiaries and most of the partner organisations took part. The first meeting contained an introductory workshop on molecular dynamics simulations, and a soft skill coaching "Teambuilding". The second meeting offered a course on fluorescence techniques, and a soft skill coaching "Open science in practice". On both meetings one day was dedicated to external invited speakers - acknowledged leaders in the field. The first scientific results will be reported in a due date in May.
PROTON will train 15 PhD students, who will acquire a solid state-of-the-art multidisciplinary scientific training in all kinds of proton migration/reaction systems, covering from basic science to industrial applications, thus preparing them to generate new scientific knowledge of the highest impact. In addition, practical training on transferable skills will increase their employability and qualify them for responsible positions in private and public sectors. Cross-disciplinary strategies and close collaboration with industry will enable them to resolve the molecular details of proton driven processes in all kinds of settings - enabling the improvement of biomimetic applications – up to fuel cells - and to identify lead substances which may serve to pharmacologically interfere with proton transport through membrane channels and transporters.