Projektbeschreibung
Echtzeitbildgebung der Fischentwicklung
Die häufige Fehlentwicklung bei Plattfischen während ihrer Metamorphose ist ein erhebliches Hindernis für ihre effektive Aquakultur. Der Übergang von der Larve zum Fisch ist zwar bereits eingehend erforscht, doch die bisherigen Methoden stützten sich hauptsächlich auf histologische Schnitte und Genexpressionsanalysen. Das EU-finanzierte Projekt OPTIMAR möchte die spezifischen molekularen Signalwege untersuchen, die mit einer anormalen Metamorphose in Zusammenhang stehen. Die Forschenden werden dazu nichtinvasive optische Bildgebungsinstrumente entwickeln, die in der Lage sind, die Mechanismen der Metamorphose in ganzen, lebenden Plattfischen direkt zu visualisieren. In Verbindung mit verschiedenen Bildrekonstruktionsverfahren wird es dann möglich sein, hochwertige Bilder zu erzeugen und Marker für normale und anormale Entwicklung zu identifizieren. Die Ergebnisse werden dazu beitragen, die Plattfisch-Aquakultur nachhaltiger und zugleich profitabler zu machen.
Ziel
Aquaculture is a sustainable means of generating high-quality fish to satisfy the growing global fish consumption. Flatfish are attractive species for aquaculture due to their high market value and consumer demand. However, abnormal development during metamorphosis is often a problem and has hampered the development of successful flatfish aquaculture. Hence, it is critical to understand the specific molecular signalling pathways associated with abnormal metamorphosis.
Larval metamorphosis has been studied using histological sections and gene expression profiling. However, the first requires sacrificing fish and the latter uses pool of larvae. Hence, there is an increasing demand for 3D imaging tools to directly visualise the mechanisms of metamorphosis in whole live flatfish. This offers a unique opportunity for optical imaging (OI) modalities, which are becoming popular for imaging targeted biological processes in model organisms (e.g. zebrafish). However, their full potential for biology and non-model organisms is being overlooked.
The vision for this fellowship is to develop a non-invasive OI tool for real-time 3D in vivo imaging, for studying the molecular mechanisms regulating flatfish metamorphosis. The project combines recent advances in the mathematics of forward and inverse problems, marine molecular biology and modern OI technologies, including selective plane illumination microscopy and optical projection tomography. The developed OI tools will provide clear high-resolution pictures from the inside of live flatfish. Accurate light propagation models and image reconstruction methods will be developed to obtain high-quality images of biological significance. Moreover, novel acquisition schemes will be implemented to enable real-time 3D imaging. Finally, the OI tools will be used for in vivo flatfish imaging to obtain markers of normal and abnormal metamorphosis. This is essential to establish a sustainable and profitable flatfish aquaculture industry.
Wissenschaftliches Gebiet
Schlüsselbegriffe
Programm/Programme
Thema/Themen
Aufforderung zur Vorschlagseinreichung
Andere Projekte für diesen Aufruf anzeigenUnterauftrag
H2020-WF-01-2018
Finanzierungsplan
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Koordinator
8005-139 FARO
Portugal