Project description
Studying the architecture of metaphase chromosomes
Chromosomes acquire their most compact state just before separation during metaphase. The EU-funded MONOCHROME project aims to develop an approach that allows real-time super-resolution imaging and manipulation of entire non-fixed metaphase chromosomes under near-physiological conditions. Researchers propose to obtain quantitative images of the proteins that establish the architecture of metaphase chromosomes and analyse the internal protein dynamics at the molecular level. The introduction of force-induced expansion microscopy, together with advanced labelling and imaging techniques, will make possible the validation and correction of the current models of metaphase organisation as well as an exploration of the physical properties of chromosomes and chromosome separation dynamics.
Fields of science
- natural scienceschemical sciencesanalytical chemistryquantitative analysis
- natural sciencesphysical sciencesopticsmicroscopysuper resolution microscopy
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteins
- medical and health sciencesclinical medicineoncology
- natural sciencesbiological sciencesgeneticschromosomes
Programme(s)
Topic(s)
Funding Scheme
ERC-ADG - Advanced Grant
Host institution
1081 HV Amsterdam
Netherlands
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Beneficiaries (1)
1081 HV Amsterdam
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