Descripción del proyecto
Dispositivo microfluídico de órganos sobre un chip para mejorar el desarrollo preclínico de fármacos
El proyecto financiado con fondos europeos PORTable STaNDOuTs tiene por objeto desarrollar dispositivos microfluídicos de órganos sobre un chip integrados con electrodos para realizar mediciones de la resistencia eléctrica transepitelial (TEER) y reemplazar los actuales procedimientos preclínicos del proceso de desarrollo de fármacos, los cuales no resultan eficientes en cuanto a tiempo y costes. La medición TEER resulta rápida y no invasiva, e indica la integridad de la barrera de la membrana celular de distintos órganos necesarios para la homeostasis del cuerpo humano. El objetivo es fabricar un dispositivo perfundido con tres compartimentos: uno para el intestino, uno para el hígado y uno para los riñones, equipados de forma independiente con electrodos para medir los valores TEER y detectar la integridad de las barreras de la membrana celular de cada compartimento orgánico. Posteriormente, la tecnología desarrollada se validará por su capacidad para estudiar la nefrotoxicidad inducida por fármacos.
Objetivo
PORTable STaNDOuTs aims to develop a multiple Organs-on-a-Chip (MOOC) microfluidic device integrated with electrodes for carrying out Transepithelial electrical resistance (TEER) measurements. The key motivation for adopting such an approach is to develop a rapid, versatile and cost-effective prototype with ability for real-time monitoring to replace the conventional long and cost-ineffective preclinical procedures involved in the drug development process. On administration of an oral pharmaceutical drug, major organs that are involved in the absorption and metabolism of drug are intestine and liver. The drug is then distributed to different target organs such as brain, heart, lung, etc. Finally, the drug metabolites are eliminated from the human system with the help of kidney. Additionally, it has also been found that certain drug metabolites produced by the liver cause non-target drug induced organ toxicity. TEER measurement is a rapid and non-invasive technique for the real-time detection of pathophysiology. TEER indicates the integrity of the cell membrane barrier of different organs that is necessary for maintaining homeostasis within the human body. In view of this, we intend to fabricate a physiologically-mimicking perfused intestine-liver-kidney microfluidic chip with provision for carrying out in-chip TEER measurements. The device will be designed to consist of three compartments for organs namely, intestine, liver and kidney, independently equipped with electrodes for measuring TEER values to detect the integrity of cell membrane barriers in each organ compartment. The developed device will be then subsequently validated for its ability to study drug-induced nephrotoxity. Furthermore, the device will also be made versatile to incorporate other organs of interest to study non-target organ toxicity. Thus, the developed MOOC microfluidic device will surely boost the drug development process that will successively improve the health security of the society.
Ámbito científico
- medical and health sciencesbasic medicinepharmacology and pharmacydrug discovery
- natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidics
- medical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugs
- medical and health sciencesbasic medicinephysiologypathophysiology
- medical and health sciencesbasic medicinephysiologyhomeostasis
Palabras clave
Programa(s)
Régimen de financiación
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinador
93100 Montreuil
Francia
Organización definida por ella misma como pequeña y mediana empresa (pyme) en el momento de la firma del acuerdo de subvención.