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Depicting the richest source of pathogen linked miscellaneous gene expression in orphan crops by Nanopore.

Descrizione del progetto

Perché una pianta di pepe è resistente alle malattie

Il Piper colubrinum, utilizzato per curare la pododermatite, è un parente selvatico delle specie coltivate di pepe nero, il Piper nigrum. Mentre il Piper colubrinum è resistente alla malattia, il Piper nigrum (una pregiata spezia) non lo è. Mancano dati confermati sulle sequenze genomiche di entrambe le specie. Inoltre, la ragione esatta della resistenza del Piper colubrinum non è stata ancora chiarita. Per trovare le risposte a tali quesiti, il progetto OCNANO finanziato dall’UE utilizzerà un dispositivo portatile in tempo reale chiamato MinION per il sequenziamento del DNA e dell’RNA. Nello specifico, il dispositivo annoterà i trascrittomi di entrambe le specie di pepe, utilizzando inoltre una cattura basata su array per individuare l’espressione genica dell’agente patogeno durante l’infezione. Il progetto metterà anche a confronto l’analisi dell’espressione genica del Piper nigrum e del Piper colubrinum durante l’infezione.

Obiettivo

Modern agriculture is dominated by crops noticeable for intense food supply leaving behind a class of neglected or orphan crops. Relatively poorly studied orphan crops have the potential to diversify the human diet, increase agricultural food productivity levels and enable more sustainable and resilient agro- and horti-food systems. Here, we focus on two such crops-Piper nigrum (black pepper) the most important spice traded internationally and Piper colubrinum, a wild relative of black pepper introduced from Brazil, which is the only known source of resistance to diseases such as foot rot or quick wilt caused by an Oomycete Phytophthora capsici. Negligible genome sequence data exists for either species. Although agricultural practices for making P. nigrum resistant to this Oomycete by interspecific hybridization with P.colubrinum are well known, no effective strategies have been generated yet. The exact reason behind the resistance of P.colubrinum is not yet understood, thus leaving open a fertile area of investigation.With this background, OCNANO aims to reveal the basis of pathogen resistance that distinguish resistant from susceptible species of Piper. We will employ Oxford nanopore MinION technology to address this issue with three specific objectives:(1) Annotate the transcriptomes of P.nigrum and P. colubrinum; (2) Identify pathogen gene expression during infection using a capture array; (3) Comparative analysis of P. nigrum and P. colubrinum gene expression during infection. Innovative strategies like nanopore in combination with other sequencing approaches can transform annotation and enable the richest understanding of gene expression during pathogen attack. Research progressing towards the rescue of such orphan crops from their environmental stresses, by applying leading edge technologies, can further enhance crop productivity, food security, knowledge based economy and society and contribute to the achievement of several UN Sustainable Development Goals.

Meccanismo di finanziamento

MSCA-IF-EF-ST - Standard EF

Coordinatore

UNIVERSITY OF DUNDEE
Contribution nette de l'UE
€ 224 933,76
Indirizzo
Nethergate
DD1 4HN Dundee
Regno Unito

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Regione
Scotland Eastern Scotland Angus and Dundee City
Tipo di attività
Higher or Secondary Education Establishments
Collegamenti
Altri finanziamenti
€ 0,00