Beyond super-resolution: ultra-resolution imaging provides solutions for synapse physiology and brain pathology

Información del proyecto

ULTRARESOLUTION

Identificador del acuerdo de subvención: 951275

DOI

10.3030/951275

Fecha de la firma de la CE 1 Marzo 2021

Fecha de inicio 1 Junio 2021

Fecha de finalización 31 Mayo 2027

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 11 100 221,00

Aportación de la UE

€ 11 100 221,00

Coordinado por

UNIVERSITAETSMEDIZIN GOETTINGEN - GEORG-AUGUST-UNIVERSITAET GOETTINGEN - STIFTUNG OEFFENTLICHEN RECHTS
Germany

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Publicaciones

Peer reviewed articles (17)

Microtubules as a versatile reference standard for expansion microscopy

Autores: Rajdeep Chowdhury, Tiago Mimoso, Abed Alrahman Chouaib, Nikolaos Mougios, Donatus Krah, Felipe Opazo, Sarah Köster, Silvio O. Rizzoli, Ali H. Shaib
Publicado en: Communications Biology, Edición 8, 2025, Página(s) USA, ISSN 2399-3642
Editor: Springer Science and Business Media LLC
DOI: 10.1038/s42003-025-07967-3

PCNA as Protein‐Based Nanoruler for Sub‐10 nm Fluorescence Imaging

Autores: Dominic A. Helmerich, Made Budiarta, Danush Taban, Sören Doose, Gerti Beliu, Markus Sauer
Publicado en: Advanced Materials, Edición 36, 2024, ISSN 0935-9648
Editor: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202310104

Convex hull as diagnostic tool in single-molecule localization microscopy.

Autores: Ebert V, Eiring P, Helmerich DA, Seifert R, Sauer M, Doose S.
Publicado en: Bioinformatics, 2022, ISSN 1367-4811
Editor: Oxford University Press
DOI: 10.1093/bioinformatics/btac700

Functionalized Docetaxel Probes for Refined Visualization of Mitotic Spindles by Expansion Microscopy

Autores: Gang Wen, Xiong Chen, Patrick Eiring, Volker Leen, Johan Hofkens, Markus Sauer
Publicado en: Journal of the American Chemical Society, Edición 147, 2025, Página(s) 6604-6611, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.4c15608

Multiplexed expansion revealing for imaging multiprotein nanostructures in healthy and diseased brain

Autores: Jinyoung Kang, Margaret E. Schroeder, Youngmi Lee, Chaitanya Kapoor, Eunah Yu, Tyler B. Tarr, Kat Titterton, Menglong Zeng, Demian Park, Emily Niederst, Donglai Wei, Guoping Feng, Edward S. Boyden
Publicado en: Nature Communications, Edición 15, 2024, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-024-53729-w

Extracellular matrix remodeling throughendocytosis and resurfacing of Tenascin-R

Autores: Dankovich TM, Kaushik R, Olsthoorn LHM, Petersen GC, Giro PE, Kluever V, Agüi-Gonzalez P, Grewe K, Bao G, Beuermann S, Hadi HA, Doeren J, Klöppner S, Cooper BH, Dityatev A, Rizzoli SO
Publicado en: Nature Communications, 2021, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-021-27462-7

ReCSAI: recursive compressed sensing artificial intelligence for confocal lifetime localization microscopy.

Autores: Reinhard S, Helmerich DA, Boras D, Sauer M, Kollmannsberger P
Publicado en: BMC Bioinformatics, 2022, ISSN 1471-2105
Editor: BioMed Central
DOI: 10.1186/s12859-022-05071-5

Visualization of F-Actin Through Expansion Microscopy (ExM) with Trifunctional Linker-Conjugated Phalloidin

Autores: Jianjun Huang, Gang Wen, Thibo Iven, Débora Linhares, Leewon Koo, Markus Sauer, Wim Dehaen, Volker Leen, Johan Hofkens
Publicado en: BIO-PROTOCOL, Edición 15, 2025, ISSN 2331-8325
Editor: Bio-Protocol, LLC
DOI: 10.21769/bioprotoc.5273

Photoswitching fingerprint analysis bypasses the 10-nm resolution barrier

Autores: Helmerich DA, Beliu G, Taban D, Meub M, Streit M, Kuhlemann A, Doose S, Sauer M
Publicado en: Nature Methods, 2022, ISSN 1548-7105
Editor: Nature Research
DOI: 10.1038/s41592-022-01548-6

Decoding the molecular interplay of CD20 and therapeutic antibodies with fast volumetric nanoscopy

Autores: Arindam Ghosh, Mara Meub, Dominic A. Helmerich, Julia Weingart, Patrick Eiring, Thomas Nerreter, K. Martin Kortüm, Sören Doose, Markus Sauer
Publicado en: Science, Edición 387, 2025, ISSN 0036-8075
Editor: American Association for the Advancement of Science
DOI: 10.1126/science.adq4510

Enhanced synaptic protein visualization by multicolor super-resolution expansion microscopy

Autores: Janna Eilts, Sebastian Reinhard, Nikolas Michetschläger, Christian Werner, Markus Sauer
Publicado en: Neurophotonics, Edición 10, 2024, ISSN 2329-423X
Editor: SPIE-Intl Soc Optical Eng
DOI: 10.1117/1.nph.10.4.044412

One-step nanoscale expansion microscopy reveals individual protein shapes

Autores: Ali H. Shaib, Abed Alrahman Chouaib, Rajdeep Chowdhury, Jonas Altendorf, Daniel Mihaylov, Chi Zhang, Donatus Krah, Vanessa Imani, Russell K. W. Spencer, Svilen Veselinov Georgiev, Nikolaos Mougios, Mehar Monga, Sofiia Reshetniak, Tiago Mimoso, Han Chen, Parisa Fatehbasharzad, Dagmar Crzan, Kim-Ann Saal, Mohamad Mahdi Alawieh, Nadia Alawar, Janna Eilts, Jinyoung Kang, Alireza Soleimani, Marcus Mül
Publicado en: Nature Biotechnology, 2024, ISSN 1087-0156
Editor: Nature Publishing Group
DOI: 10.1038/s41587-024-02431-9

Single-shot 20-fold expansion microscopy

Autores: Shiwei Wang, Tay Won Shin, Harley B. Yoder, Ryan B. McMillan, Hanquan Su, Yixi Liu, Chi Zhang, Kylie S. Leung, Peng Yin, Laura L. Kiessling, Edward S. Boyden
Publicado en: Nature Methods, Edición 21, 2024, Página(s) 2128-2134, ISSN 1548-7091
Editor: Nature Publishing Group
DOI: 10.1038/s41592-024-02454-9

Coronaviruses Use ACE2 Monomers as Entry-Receptors

Autores: Eiring P, Klein T, Backes S, Streit M, Jungblut M, Doose S, Beliu G, Sauer M.
Publicado en: Angew Chem Int Ed Engl, 2023, ISSN 1521-3757
Editor: Society of German Chemists
DOI: 10.1002/anie.202300821

Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons.

Autores: Diogo Bessa-Neto; Gerti Beliu; Alexander Kuhlemann; Valeria Pecoraro; Sören Doose; Natacha Retailleau; Nicolas Chevrier; David Perrais; Markus Sauer; Daniel Choquet
Publicado en: "Nature Communications, Nature Publishing Group, 2021, 12 (1), ⟨10.1038/s41467-021-27025-w⟩", Edición 1, 2021, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-021-27025-w

Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy

Autores: Jan Christoph Thiele; Oleksii Nevskyi; Dominic A. Helmerich; Markus Sauer; Jörg Enderlein; Jörg Enderlein
Publicado en: Frontiers in Bioinformatics, Vol 1 (2021), Edición 1, 2021, ISSN 2673-7647
Editor: Frontiers
DOI: 10.3389/fbinf.2021.740281

Heat denaturation enables multicolor X10-STED microscopy.

Autores: Saal KA, Shaib AH, Mougios N, Crzan D, Opazo F, Rizzoli SO
Publicado en: Scientific Reports, 2023, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-023-32524-5

Other (3)

Visualizing proteins by expansion microscopy.

Autores: Ali H. Shaib, Abed Alrahman Chouaib, Rajdeep Chowdhury, Daniel Mihaylov, Chi Zhang, Vanessa Imani, Svilen Veselinov Georgiev, Nikolaos Mougios, Mehar Monga, Sofiia Reshetniak, Tiago Mimoso, Han Chen, Parisa Fatehbasharzad, Dagmar Crzan, Kim-Ann Saal, Nadia Alawar, Janna Eilts, Jinyoung Kang, Luis Alvarez, Claudia Trenkwalder, Brit Mollenhauer, Tiago F. Outeiro, Sarah Köster, Julia Preobraschenski
Publicado en: BioRxiv, 2023
Editor: BioRxiv
DOI: 10.1101/2022.08.03.502284

A Multifunctional Anchor for Multimodal Expansion Microscopy.

Autores: Yi Cui, Gaojie Yang, Daniel R. Goodwin, Ciara H. O’Flanagan, Anubhav Sinha, Chi Zhang, Kristina E. Kitko, Demian Park, Samuel Aparicio, IMAXT Consortium, Edward S. Boyden
Publicado en: BioRxiv, 2022
Editor: BioRxiv
DOI: 10.1101/2022.06.19.496699

Trifunctional linkers enable improved visualization of actin by expansion microscopy.

Autores: G Wen, MD Lycas, Y Jia, V Leen, M Sauer, J Hofkens
Publicado en: BioRxiv, 2023
Editor: BioRxiv
DOI: 10.1101/2023.05.10.540261v1