Beyond super-resolution: ultra-resolution imaging provides solutions for synapse physiology and brain pathology

Informazioni relative al progetto

ULTRARESOLUTION

ID dell’accordo di sovvenzione: 951275

DOI

10.3030/951275

Data della firma CE 1 Marzo 2021

Data di avvio 1 Giugno 2021

Data di completamento 31 Maggio 2027

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 11 100 221,00

Contributo UE

€ 11 100 221,00

Coordinato da

UNIVERSITAETSMEDIZIN GOETTINGEN - GEORG-AUGUST-UNIVERSITAET GOETTINGEN - STIFTUNG OEFFENTLICHEN RECHTS
Germany

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Pubblicazioni

Peer reviewed articles (17)

Microtubules as a versatile reference standard for expansion microscopy

Autori: Rajdeep Chowdhury, Tiago Mimoso, Abed Alrahman Chouaib, Nikolaos Mougios, Donatus Krah, Felipe Opazo, Sarah Köster, Silvio O. Rizzoli, Ali H. Shaib
Pubblicato in: Communications Biology, Numero 8, 2025, Pagina/e USA, ISSN 2399-3642
Editore: Springer Science and Business Media LLC
DOI: 10.1038/s42003-025-07967-3

PCNA as Protein‐Based Nanoruler for Sub‐10 nm Fluorescence Imaging

Autori: Dominic A. Helmerich, Made Budiarta, Danush Taban, Sören Doose, Gerti Beliu, Markus Sauer
Pubblicato in: Advanced Materials, Numero 36, 2024, ISSN 0935-9648
Editore: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202310104

Convex hull as diagnostic tool in single-molecule localization microscopy.

Autori: Ebert V, Eiring P, Helmerich DA, Seifert R, Sauer M, Doose S.
Pubblicato in: Bioinformatics, 2022, ISSN 1367-4811
Editore: Oxford University Press
DOI: 10.1093/bioinformatics/btac700

Functionalized Docetaxel Probes for Refined Visualization of Mitotic Spindles by Expansion Microscopy

Autori: Gang Wen, Xiong Chen, Patrick Eiring, Volker Leen, Johan Hofkens, Markus Sauer
Pubblicato in: Journal of the American Chemical Society, Numero 147, 2025, Pagina/e 6604-6611, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.4c15608

Multiplexed expansion revealing for imaging multiprotein nanostructures in healthy and diseased brain

Autori: Jinyoung Kang, Margaret E. Schroeder, Youngmi Lee, Chaitanya Kapoor, Eunah Yu, Tyler B. Tarr, Kat Titterton, Menglong Zeng, Demian Park, Emily Niederst, Donglai Wei, Guoping Feng, Edward S. Boyden
Pubblicato in: Nature Communications, Numero 15, 2024, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-024-53729-w

Extracellular matrix remodeling throughendocytosis and resurfacing of Tenascin-R

Autori: Dankovich TM, Kaushik R, Olsthoorn LHM, Petersen GC, Giro PE, Kluever V, Agüi-Gonzalez P, Grewe K, Bao G, Beuermann S, Hadi HA, Doeren J, Klöppner S, Cooper BH, Dityatev A, Rizzoli SO
Pubblicato in: Nature Communications, 2021, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-021-27462-7

ReCSAI: recursive compressed sensing artificial intelligence for confocal lifetime localization microscopy.

Autori: Reinhard S, Helmerich DA, Boras D, Sauer M, Kollmannsberger P
Pubblicato in: BMC Bioinformatics, 2022, ISSN 1471-2105
Editore: BioMed Central
DOI: 10.1186/s12859-022-05071-5

Visualization of F-Actin Through Expansion Microscopy (ExM) with Trifunctional Linker-Conjugated Phalloidin

Autori: Jianjun Huang, Gang Wen, Thibo Iven, Débora Linhares, Leewon Koo, Markus Sauer, Wim Dehaen, Volker Leen, Johan Hofkens
Pubblicato in: BIO-PROTOCOL, Numero 15, 2025, ISSN 2331-8325
Editore: Bio-Protocol, LLC
DOI: 10.21769/bioprotoc.5273

Photoswitching fingerprint analysis bypasses the 10-nm resolution barrier

Autori: Helmerich DA, Beliu G, Taban D, Meub M, Streit M, Kuhlemann A, Doose S, Sauer M
Pubblicato in: Nature Methods, 2022, ISSN 1548-7105
Editore: Nature Research
DOI: 10.1038/s41592-022-01548-6

Decoding the molecular interplay of CD20 and therapeutic antibodies with fast volumetric nanoscopy

Autori: Arindam Ghosh, Mara Meub, Dominic A. Helmerich, Julia Weingart, Patrick Eiring, Thomas Nerreter, K. Martin Kortüm, Sören Doose, Markus Sauer
Pubblicato in: Science, Numero 387, 2025, ISSN 0036-8075
Editore: American Association for the Advancement of Science
DOI: 10.1126/science.adq4510

Enhanced synaptic protein visualization by multicolor super-resolution expansion microscopy

Autori: Janna Eilts, Sebastian Reinhard, Nikolas Michetschläger, Christian Werner, Markus Sauer
Pubblicato in: Neurophotonics, Numero 10, 2024, ISSN 2329-423X
Editore: SPIE-Intl Soc Optical Eng
DOI: 10.1117/1.nph.10.4.044412

One-step nanoscale expansion microscopy reveals individual protein shapes

Autori: Ali H. Shaib, Abed Alrahman Chouaib, Rajdeep Chowdhury, Jonas Altendorf, Daniel Mihaylov, Chi Zhang, Donatus Krah, Vanessa Imani, Russell K. W. Spencer, Svilen Veselinov Georgiev, Nikolaos Mougios, Mehar Monga, Sofiia Reshetniak, Tiago Mimoso, Han Chen, Parisa Fatehbasharzad, Dagmar Crzan, Kim-Ann Saal, Mohamad Mahdi Alawieh, Nadia Alawar, Janna Eilts, Jinyoung Kang, Alireza Soleimani, Marcus Mül
Pubblicato in: Nature Biotechnology, 2024, ISSN 1087-0156
Editore: Nature Publishing Group
DOI: 10.1038/s41587-024-02431-9

Single-shot 20-fold expansion microscopy

Autori: Shiwei Wang, Tay Won Shin, Harley B. Yoder, Ryan B. McMillan, Hanquan Su, Yixi Liu, Chi Zhang, Kylie S. Leung, Peng Yin, Laura L. Kiessling, Edward S. Boyden
Pubblicato in: Nature Methods, Numero 21, 2024, Pagina/e 2128-2134, ISSN 1548-7091
Editore: Nature Publishing Group
DOI: 10.1038/s41592-024-02454-9

Coronaviruses Use ACE2 Monomers as Entry-Receptors

Autori: Eiring P, Klein T, Backes S, Streit M, Jungblut M, Doose S, Beliu G, Sauer M.
Pubblicato in: Angew Chem Int Ed Engl, 2023, ISSN 1521-3757
Editore: Society of German Chemists
DOI: 10.1002/anie.202300821

Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons.

Autori: Diogo Bessa-Neto; Gerti Beliu; Alexander Kuhlemann; Valeria Pecoraro; Sören Doose; Natacha Retailleau; Nicolas Chevrier; David Perrais; Markus Sauer; Daniel Choquet
Pubblicato in: "Nature Communications, Nature Publishing Group, 2021, 12 (1), ⟨10.1038/s41467-021-27025-w⟩", Numero 1, 2021, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-021-27025-w

Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy

Autori: Jan Christoph Thiele; Oleksii Nevskyi; Dominic A. Helmerich; Markus Sauer; Jörg Enderlein; Jörg Enderlein
Pubblicato in: Frontiers in Bioinformatics, Vol 1 (2021), Numero 1, 2021, ISSN 2673-7647
Editore: Frontiers
DOI: 10.3389/fbinf.2021.740281

Heat denaturation enables multicolor X10-STED microscopy.

Autori: Saal KA, Shaib AH, Mougios N, Crzan D, Opazo F, Rizzoli SO
Pubblicato in: Scientific Reports, 2023, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-023-32524-5

Other (3)

Visualizing proteins by expansion microscopy.

Autori: Ali H. Shaib, Abed Alrahman Chouaib, Rajdeep Chowdhury, Daniel Mihaylov, Chi Zhang, Vanessa Imani, Svilen Veselinov Georgiev, Nikolaos Mougios, Mehar Monga, Sofiia Reshetniak, Tiago Mimoso, Han Chen, Parisa Fatehbasharzad, Dagmar Crzan, Kim-Ann Saal, Nadia Alawar, Janna Eilts, Jinyoung Kang, Luis Alvarez, Claudia Trenkwalder, Brit Mollenhauer, Tiago F. Outeiro, Sarah Köster, Julia Preobraschenski
Pubblicato in: BioRxiv, 2023
Editore: BioRxiv
DOI: 10.1101/2022.08.03.502284

A Multifunctional Anchor for Multimodal Expansion Microscopy.

Autori: Yi Cui, Gaojie Yang, Daniel R. Goodwin, Ciara H. O’Flanagan, Anubhav Sinha, Chi Zhang, Kristina E. Kitko, Demian Park, Samuel Aparicio, IMAXT Consortium, Edward S. Boyden
Pubblicato in: BioRxiv, 2022
Editore: BioRxiv
DOI: 10.1101/2022.06.19.496699

Trifunctional linkers enable improved visualization of actin by expansion microscopy.

Autori: G Wen, MD Lycas, Y Jia, V Leen, M Sauer, J Hofkens
Pubblicato in: BioRxiv, 2023
Editore: BioRxiv
DOI: 10.1101/2023.05.10.540261v1