Beyond super-resolution: ultra-resolution imaging provides solutions for synapse physiology and brain pathology

Project Information

ULTRARESOLUTION

Grant agreement ID: 951275

DOI

10.3030/951275

EC signature date 1 March 2021

Start date 1 June 2021

End date 31 May 2027

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 11 100 221,00

EU contribution

€ 11 100 221,00

Coordinated by

UNIVERSITAETSMEDIZIN GOETTINGEN - GEORG-AUGUST-UNIVERSITAET GOETTINGEN - STIFTUNG OEFFENTLICHEN RECHTS
Germany

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (17)

Microtubules as a versatile reference standard for expansion microscopy

Author(s): Rajdeep Chowdhury, Tiago Mimoso, Abed Alrahman Chouaib, Nikolaos Mougios, Donatus Krah, Felipe Opazo, Sarah Köster, Silvio O. Rizzoli, Ali H. Shaib
Published in: Communications Biology, Issue 8, 2025, Page(s) USA, ISSN 2399-3642
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s42003-025-07967-3

PCNA as Protein‐Based Nanoruler for Sub‐10 nm Fluorescence Imaging

Author(s): Dominic A. Helmerich, Made Budiarta, Danush Taban, Sören Doose, Gerti Beliu, Markus Sauer
Published in: Advanced Materials, Issue 36, 2024, ISSN 0935-9648
Publisher: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202310104

Convex hull as diagnostic tool in single-molecule localization microscopy.

Author(s): Ebert V, Eiring P, Helmerich DA, Seifert R, Sauer M, Doose S.
Published in: Bioinformatics, 2022, ISSN 1367-4811
Publisher: Oxford University Press
DOI: 10.1093/bioinformatics/btac700

Functionalized Docetaxel Probes for Refined Visualization of Mitotic Spindles by Expansion Microscopy

Author(s): Gang Wen, Xiong Chen, Patrick Eiring, Volker Leen, Johan Hofkens, Markus Sauer
Published in: Journal of the American Chemical Society, Issue 147, 2025, Page(s) 6604-6611, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.4c15608

Multiplexed expansion revealing for imaging multiprotein nanostructures in healthy and diseased brain

Author(s): Jinyoung Kang, Margaret E. Schroeder, Youngmi Lee, Chaitanya Kapoor, Eunah Yu, Tyler B. Tarr, Kat Titterton, Menglong Zeng, Demian Park, Emily Niederst, Donglai Wei, Guoping Feng, Edward S. Boyden
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-53729-w

Extracellular matrix remodeling throughendocytosis and resurfacing of Tenascin-R

Author(s): Dankovich TM, Kaushik R, Olsthoorn LHM, Petersen GC, Giro PE, Kluever V, Agüi-Gonzalez P, Grewe K, Bao G, Beuermann S, Hadi HA, Doeren J, Klöppner S, Cooper BH, Dityatev A, Rizzoli SO
Published in: Nature Communications, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-27462-7

ReCSAI: recursive compressed sensing artificial intelligence for confocal lifetime localization microscopy.

Author(s): Reinhard S, Helmerich DA, Boras D, Sauer M, Kollmannsberger P
Published in: BMC Bioinformatics, 2022, ISSN 1471-2105
Publisher: BioMed Central
DOI: 10.1186/s12859-022-05071-5

Visualization of F-Actin Through Expansion Microscopy (ExM) with Trifunctional Linker-Conjugated Phalloidin

Author(s): Jianjun Huang, Gang Wen, Thibo Iven, Débora Linhares, Leewon Koo, Markus Sauer, Wim Dehaen, Volker Leen, Johan Hofkens
Published in: BIO-PROTOCOL, Issue 15, 2025, ISSN 2331-8325
Publisher: Bio-Protocol, LLC
DOI: 10.21769/bioprotoc.5273

Photoswitching fingerprint analysis bypasses the 10-nm resolution barrier

Author(s): Helmerich DA, Beliu G, Taban D, Meub M, Streit M, Kuhlemann A, Doose S, Sauer M
Published in: Nature Methods, 2022, ISSN 1548-7105
Publisher: Nature Research
DOI: 10.1038/s41592-022-01548-6

Decoding the molecular interplay of CD20 and therapeutic antibodies with fast volumetric nanoscopy

Author(s): Arindam Ghosh, Mara Meub, Dominic A. Helmerich, Julia Weingart, Patrick Eiring, Thomas Nerreter, K. Martin Kortüm, Sören Doose, Markus Sauer
Published in: Science, Issue 387, 2025, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.adq4510

Enhanced synaptic protein visualization by multicolor super-resolution expansion microscopy

Author(s): Janna Eilts, Sebastian Reinhard, Nikolas Michetschläger, Christian Werner, Markus Sauer
Published in: Neurophotonics, Issue 10, 2024, ISSN 2329-423X
Publisher: SPIE-Intl Soc Optical Eng
DOI: 10.1117/1.nph.10.4.044412

One-step nanoscale expansion microscopy reveals individual protein shapes

Author(s): Ali H. Shaib, Abed Alrahman Chouaib, Rajdeep Chowdhury, Jonas Altendorf, Daniel Mihaylov, Chi Zhang, Donatus Krah, Vanessa Imani, Russell K. W. Spencer, Svilen Veselinov Georgiev, Nikolaos Mougios, Mehar Monga, Sofiia Reshetniak, Tiago Mimoso, Han Chen, Parisa Fatehbasharzad, Dagmar Crzan, Kim-Ann Saal, Mohamad Mahdi Alawieh, Nadia Alawar, Janna Eilts, Jinyoung Kang, Alireza Soleimani, Marcus Mül
Published in: Nature Biotechnology, 2024, ISSN 1087-0156
Publisher: Nature Publishing Group
DOI: 10.1038/s41587-024-02431-9

Single-shot 20-fold expansion microscopy

Author(s): Shiwei Wang, Tay Won Shin, Harley B. Yoder, Ryan B. McMillan, Hanquan Su, Yixi Liu, Chi Zhang, Kylie S. Leung, Peng Yin, Laura L. Kiessling, Edward S. Boyden
Published in: Nature Methods, Issue 21, 2024, Page(s) 2128-2134, ISSN 1548-7091
Publisher: Nature Publishing Group
DOI: 10.1038/s41592-024-02454-9

Coronaviruses Use ACE2 Monomers as Entry-Receptors

Author(s): Eiring P, Klein T, Backes S, Streit M, Jungblut M, Doose S, Beliu G, Sauer M.
Published in: Angew Chem Int Ed Engl, 2023, ISSN 1521-3757
Publisher: Society of German Chemists
DOI: 10.1002/anie.202300821

Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons.

Author(s): Diogo Bessa-Neto; Gerti Beliu; Alexander Kuhlemann; Valeria Pecoraro; Sören Doose; Natacha Retailleau; Nicolas Chevrier; David Perrais; Markus Sauer; Daniel Choquet
Published in: "Nature Communications, Nature Publishing Group, 2021, 12 (1), ⟨10.1038/s41467-021-27025-w⟩", Issue 1, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-27025-w

Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy

Author(s): Jan Christoph Thiele; Oleksii Nevskyi; Dominic A. Helmerich; Markus Sauer; Jörg Enderlein; Jörg Enderlein
Published in: Frontiers in Bioinformatics, Vol 1 (2021), Issue 1, 2021, ISSN 2673-7647
Publisher: Frontiers
DOI: 10.3389/fbinf.2021.740281

Heat denaturation enables multicolor X10-STED microscopy.

Author(s): Saal KA, Shaib AH, Mougios N, Crzan D, Opazo F, Rizzoli SO
Published in: Scientific Reports, 2023, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-023-32524-5

Other (3)

Visualizing proteins by expansion microscopy.

Author(s): Ali H. Shaib, Abed Alrahman Chouaib, Rajdeep Chowdhury, Daniel Mihaylov, Chi Zhang, Vanessa Imani, Svilen Veselinov Georgiev, Nikolaos Mougios, Mehar Monga, Sofiia Reshetniak, Tiago Mimoso, Han Chen, Parisa Fatehbasharzad, Dagmar Crzan, Kim-Ann Saal, Nadia Alawar, Janna Eilts, Jinyoung Kang, Luis Alvarez, Claudia Trenkwalder, Brit Mollenhauer, Tiago F. Outeiro, Sarah Köster, Julia Preobraschenski
Published in: BioRxiv, 2023
Publisher: BioRxiv
DOI: 10.1101/2022.08.03.502284

A Multifunctional Anchor for Multimodal Expansion Microscopy.

Author(s): Yi Cui, Gaojie Yang, Daniel R. Goodwin, Ciara H. O’Flanagan, Anubhav Sinha, Chi Zhang, Kristina E. Kitko, Demian Park, Samuel Aparicio, IMAXT Consortium, Edward S. Boyden
Published in: BioRxiv, 2022
Publisher: BioRxiv
DOI: 10.1101/2022.06.19.496699

Trifunctional linkers enable improved visualization of actin by expansion microscopy.

Author(s): G Wen, MD Lycas, Y Jia, V Leen, M Sauer, J Hofkens
Published in: BioRxiv, 2023
Publisher: BioRxiv
DOI: 10.1101/2023.05.10.540261v1