RISK assessment of chemicals integrating HUman centric Next generation Testing strategies promoting the 3Rs

Project Information

RISK-HUNT3R

Grant agreement ID: 964537

DOI 10.3030/964537

EC signature date 22 January 2021

Start date 1 June 2021

End date 31 May 2026

Funded under

• SOCIETAL CHALLENGES - Health, demographic change and well-being

Total cost € 23 177 768,75

EU contribution € 22 927 768,75

Coordinated by

UNIVERSITEIT LEIDEN
Netherlands

Publications

Peer reviewed articles (24)

SimRFlow: An R-based workflow for automated high-throughput PBPK simulation with the Simcyp® simulator

Author(s): Khalidi H, Onasanwo A, Islam B, Jo H, Fisher C, Aidley R, Gardner I, Bois FY.
Published in: Frontiers in Pharmacology, Issue 25 August 2022, 2022, ISSN 1663-9812
Publisher: Frontiers Media S.A.
DOI: 10.3389/fphar.2022.929200

Generation of Human Nociceptor-Enriched Sensory Neurons for the Study of Pain-Related Dysfunctions

Author(s): Holzer, A. K., Karreman, C., Suciu, I., Furmanowsky, L. S., Wohlfarth, H., Loser, D., Dirks, W. G., Pardo González, E., and Leist, M.
Published in: Stem Cells Transl Med., Issue 11(7), July 2022, 2022, Page(s) 727–741, ISSN 0029-3970
Publisher: Oxford University Press
DOI: 10.1093/stcltm/szac031

A framework for chemical safety assessment incorporating new approach methodologies within REACH

Author(s): Ball N, Bars R, Botham PA, Cuciureanu A, Cronin MTD, Doe JE, Dudzina T, Gant TW, Leist M, van Ravenzwaay B
Published in: Arch Toxicol., 2022, ISSN 2193-1801
Publisher: Springer Science and Business Media Deutschland GmbH
DOI: 10.1007/s00204-021-03215-9

Next-generation risk assessment of chemicals – Rolling out a human-centric testing strategy to drive 3R implementation: The RISK-HUNT3R project perspective

Author(s): Pallocca G., Moné J. M., Kamp H., Luijten M., van de Water B., Leist M.
Published in: Altex, Issue 39 (2), 7 April 2022, 2022, ISSN 2193-1801
Publisher: Springer Science and Business Media Deutschland GmbH
DOI: 10.14573/altex.2204051

Profiling of Human Neural Crest Chemoattractant Activity as a Replacement of Fetal Bovine Serum for In Vitro Chemotaxis Assays

Author(s): Dolde X, Karreman C, Wiechers M, Schildknecht S, Leist M
Published in: International Journal of Molecular Sciences, Issue 22(18):10079, 2021, ISSN 2078-2489
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms221810079

Mapping the cellular response to electron transport chain inhibitors reveals selective signaling networks triggered by mitochondrial perturbation

Author(s): van der Stel W, Yang H, Vrijenhoek NG, Schimming JP, Callegaro G, Carta G, Darici S, Delp J, Forsby A, White A, le Dévédec S, Leist M, Jennings P, Beltman JB, van de Water B, Danen EHJ
Published in: Archives of Toxicology, Issue 96(1), January 2022, 2022, ISSN 1868-8551
Publisher: Springer-Spektrum
DOI: 10.1007/s00204-021-03160-7

Specific Attenuation of Purinergic Signaling during Bortezomib-Induced Peripheral Neuropathy In Vitro

Author(s): Holzer, Anna-Katharina, Ilinca Suciu, Christiaan Karreman, Thomas Goj, and Marcel Leist
Published in: International Journal of Molecular Sciences, Issue 23(7), 29 March 2022, 2022, ISSN 2078-2489
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23073734

Current Status and Challenges of Human Induced Pluripotent Stem Cell-Derived Liver Models in Drug Discovery

Author(s): Tricot T, Verfaillie C, Kumar M
Published in: Cells, Issue january 27, 2022; 11: 442, 2022, ISSN 2078-2489
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cells11030442

Establishment of a human cell-based in vitro battery to assess developmental neurotoxicity hazard of chemicals

Author(s): Blum J, Masjosthusmann S, Bartmann K, Bendt F, Dolde X, Dönmez A, Förster N, Holzer AK, Hübenthal U, Keßel HE, Kilic S, Klose J, Pahl M, Stürzl LC, Mangas I, Terron A, Crofton KM, Scholze M, Mosig A, Leist M, Fritsche E.
Published in: Chemosphere, Issue 31;311(Pt 2):137035, 2022, ISSN 1879-1298
Publisher: Elsevier
DOI: 10.1016/j.chemosphere.2022.137035

Optimization of the TeraTox Assay for Preclinical Teratogenicity Assessment

Author(s): Jaklin M, Zhang JD, Schäfer N, Clemann N, Barrow P, Küng E, Sach-Peltason L, McGinnis C, Leist M, Kustermann S.
Published in: Toxicological Sciences, Issue 188(1):17-33, 2022, ISSN 0029-3970
Publisher: Oxford University Press
DOI: 10.1093/toxsci/kfac046

Circulating (Poly)phenol Metabolites: Neuroprotection in a 3D Cell Model of Parkinson’s Disease

Author(s): Carecho R, Figueira I, Terrasso AP, Godinho-Pereira J, de Oliveira Sequeira C, Pereira SA, Milenkovic D, Leist M, Brito C, Nunes Dos Santos C.
Published in: Mol. Nutr. Food Res., Issue 29 December 2021, 2021, ISSN 1613-4125
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/mnfr.202100959

Mapping the dynamics of Nrf2 antioxidant and NFκB inflammatory responses by soft electrophilic chemicals in human liver cells defines the transition from adaptive to adverse responses

Author(s): Ter Braak B, Klip JE, Wink S, Hiemstra S, Cooper SL, Middleton A, White A, van de Water B
Published in: Toxicology In Vitro, 2022, ISSN 1879-1298
Publisher: Elsevier
DOI: 10.1016/j.tiv.2022.105419

Acute effects of the imidacloprid metabolite desnitro-imidacloprid on human nACh receptors relevant for neuronal signaling

Author(s): Loser D, Grillberger K, Hinojosa MG, Blum J, Haufe Y, Danker T, Johansson Y, Möller C, Nicke A, Bennekou SH, Gardner I, Bauch C, Walker P, Forsby A, Ecker GF, Kraushaar U, Leist M.
Published in: Arch Toxicol, Issue 95(12).3695-3716, 2021, ISSN 2193-1801
Publisher: Springer Science and Business Media Deutschland GmbH
DOI: 10.1007/s00204-021-03168-z

Transcriptomic-based evaluation of trichloroethylene glutathione and cysteine conjugates demonstrate phenotype-dependent stress responses in a panel of human in vitro models

Author(s): Capinha L, Zhang Y, Holzer AK, Ückert AK, Zana M, Carta G, Murphy C, Baldovini J, Mazidi Z, Grillari J, Dinnyes A, van de Water B, Leist M, Commandeur JNM, Jennings P
Published in: Archives of Toxicology, 2023, ISSN 2193-1801
Publisher: Springer Science and Business Media Deutschland GmbH
DOI: 10.1007/s00204-022-03436-6

Studying Metabolism by NMR-Based Metabolomics

Author(s): Sofia Moco
Published in: Frontiers Molecular Biosciences, Issue 9:882487, 2022, 2022, ISSN 1663-9812
Publisher: Frontiers Media S.A.
DOI: 10.3389/fmolb.2022.882487

Metabolic regulation by p53 prevents R-loop-associated genomic instability

Author(s): Emanuele Panatta, Alessio Butera, Eleonora Mammarella, Consuelo Pitolli, Alessandro Mauriello, Marcel Leist, Richard A. Knight, Gerry Melino, Ivano Amelio
Published in: Cell Reports, Issue Volume 41, Issue 5, 1 November 2022, 2022, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2022.111568

High Accuracy Classification of Developmental Toxicants by In Vitro Tests of Human Neuroepithelial and Cardiomyoblast Differentiation

Author(s): Seidel F, Cherianidou A, Kappenberg F, Marta M, Dreser N, Blum J, Waldmann T, Blüthgen N, Meisig J, Madjar K, Henry M, Rotshteyn T, Scholtz-Illigens A, Marchan R, Edlund K, Leist M, Rahnenführer J, Sachinidis A, Hengstler JG.
Published in: Cells, Issue 11(21):3404, 2022, ISSN 2078-2489
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cells11213404

Dynamic Metabolic and Transcriptional Responses of Proteasome-Inhibited Neurons

Author(s): Suciu, I.; Delp, J.; Gutbier, S.; Ückert, A.-K.; Spreng, A.-S.; Eberhard, P.; Karreman, C.; Schreiber, F.; Madjar, K.; Rahnenführer, J.; Celardo, I.; Amelio, I.; Leist, M
Published in: Antioxidants, 2023, ISSN 2078-2489
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/antiox12010164

Distinct and Dynamic Transcriptome Adaptations of iPSC-Generated Astrocytes after Cytokine Stimulation

Author(s): Spreng AS, Brüll M, Leisner H, Suciu I, Leist M.
Published in: Human Stem Cells in Disease Modelling, Drug Discovery and Regenerative Medicine, Issue 11(17):2644, 25 August 2022, 2022, ISSN 2078-2489
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cells11172644

Use of metabolic glycoengineering and pharmacological inhibitors to assess lipid and protein sialylation on cells.

Author(s): Kranaster P, Blum J, Dold JEGA, Wittmann V, Leist M.
Published in: Neurochem, 2022, ISSN 1613-4125
Publisher: John Wiley & Sons Ltd.
DOI: 10.1111/jnc.15737

Large-scale perfused tissues via synthetic 3D soft microfluidics

Author(s): Grebenyuk S, Abdel Fattah AR, Kumar M, Toprakhisar B, Rustandi G, Vananroye A, Salmon I, Verfaillie C, Grillo M, Ranga A
Published in: Nat Commun, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-35619-1

On the Usefulness of Animals as a Model System (Part II): Considering Benefits within Distinct Use Domains

Author(s): G. Pallocca, M. Leist
Published in: ALTEX- Alternatives to Animal Experimentation, Issue 39(3), 20 July 2022, 2022, ISSN 1868-8551
Publisher: Springer Spektrum
DOI: 10.14573/altex.2207111

Synuclein Family Members Prevent Membrane Damage by Counteracting α-Synuclein Aggregation

Author(s): Scheibe C, Karreman C, Schildknecht S, Leist M, Hauser K.
Published in: Biomolecules, Issue 11(8): 1067, Aug 2021, 2021, ISSN 2078-2489
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/biom11081067

Classification of Developmental Toxicants in a Human iPSC Transcriptomics-Based Test

Author(s): Cherianidou A, Seidel F, Kappenberg F, Dreser N, Blum J, Waldmann T, Blüthgen N, Meisig J, Madjar K, Henry M, Rotshteyn T, Marchan R, Edlund K, Leist M, Rahnenführer J, Sachinidis A, Hengstler JG.
Published in: Chemical Research in Toxicology, 2022, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/acs.chemrestox.1c00392

Non-peer reviewed articles (2)

Guidance document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0

Author(s): Pamies D, Leist M, Coecke S, Bowe G, Allen DG, Gstraunthaler G, Bal-Price A, Pistollato F, de Vries RBM, Hogberg HT, Hartung T, Stacey G.
Published in: ALTEX, Issue 39:30-70, 2022, ISSN 1868-8551
Publisher: Springer Spektrum
DOI: 10.14573/altex.2111011

On the usefulness of animals as a model system (part I): Overview of criteria and focus on robustness

Author(s): Pallocca G., Rovida C., Leist M
Published in: ALTEX, Issue 2022;39(2):347-353, 2022, ISSN 2193-1801
Publisher: Springer Science and Business Media Deutschland GmbH
DOI: 10.14573/altex.2203291