RISK assessment of chemicals integrating HUman centric Next generation Testing strategies promoting the 3Rs

Informazioni relative al progetto

RISK-HUNT3R

ID dell’accordo di sovvenzione: 964537

DOI 10.3030/964537

Data della firma CE 22 Gennaio 2021

Data di avvio 1 Giugno 2021

Data di completamento 31 Maggio 2026

Finanziato da

• SOCIETAL CHALLENGES - Health, demographic change and well-being

Costo totale € 23 177 768,75

Contributo UE € 22 927 768,75

Coordinato da

UNIVERSITEIT LEIDEN
Netherlands

Pubblicazioni

Peer reviewed articles (24)

SimRFlow: An R-based workflow for automated high-throughput PBPK simulation with the Simcyp® simulator

Autori: Khalidi H, Onasanwo A, Islam B, Jo H, Fisher C, Aidley R, Gardner I, Bois FY.
Pubblicato in: Frontiers in Pharmacology, Numero 25 August 2022, 2022, ISSN 1663-9812
Editore: Frontiers Media S.A.
DOI: 10.3389/fphar.2022.929200

Generation of Human Nociceptor-Enriched Sensory Neurons for the Study of Pain-Related Dysfunctions

Autori: Holzer, A. K., Karreman, C., Suciu, I., Furmanowsky, L. S., Wohlfarth, H., Loser, D., Dirks, W. G., Pardo González, E., and Leist, M.
Pubblicato in: Stem Cells Transl Med., Numero 11(7), July 2022, 2022, Pagina/e 727–741, ISSN 0029-3970
Editore: Oxford University Press
DOI: 10.1093/stcltm/szac031

A framework for chemical safety assessment incorporating new approach methodologies within REACH

Autori: Ball N, Bars R, Botham PA, Cuciureanu A, Cronin MTD, Doe JE, Dudzina T, Gant TW, Leist M, van Ravenzwaay B
Pubblicato in: Arch Toxicol., 2022, ISSN 2193-1801
Editore: Springer Science and Business Media Deutschland GmbH
DOI: 10.1007/s00204-021-03215-9

Next-generation risk assessment of chemicals – Rolling out a human-centric testing strategy to drive 3R implementation: The RISK-HUNT3R project perspective

Autori: Pallocca G., Moné J. M., Kamp H., Luijten M., van de Water B., Leist M.
Pubblicato in: Altex, Numero 39 (2), 7 April 2022, 2022, ISSN 2193-1801
Editore: Springer Science and Business Media Deutschland GmbH
DOI: 10.14573/altex.2204051

Profiling of Human Neural Crest Chemoattractant Activity as a Replacement of Fetal Bovine Serum for In Vitro Chemotaxis Assays

Autori: Dolde X, Karreman C, Wiechers M, Schildknecht S, Leist M
Pubblicato in: International Journal of Molecular Sciences, Numero 22(18):10079, 2021, ISSN 2078-2489
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms221810079

Mapping the cellular response to electron transport chain inhibitors reveals selective signaling networks triggered by mitochondrial perturbation

Autori: van der Stel W, Yang H, Vrijenhoek NG, Schimming JP, Callegaro G, Carta G, Darici S, Delp J, Forsby A, White A, le Dévédec S, Leist M, Jennings P, Beltman JB, van de Water B, Danen EHJ
Pubblicato in: Archives of Toxicology, Numero 96(1), January 2022, 2022, ISSN 1868-8551
Editore: Springer-Spektrum
DOI: 10.1007/s00204-021-03160-7

Specific Attenuation of Purinergic Signaling during Bortezomib-Induced Peripheral Neuropathy In Vitro

Autori: Holzer, Anna-Katharina, Ilinca Suciu, Christiaan Karreman, Thomas Goj, and Marcel Leist
Pubblicato in: International Journal of Molecular Sciences, Numero 23(7), 29 March 2022, 2022, ISSN 2078-2489
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23073734

Current Status and Challenges of Human Induced Pluripotent Stem Cell-Derived Liver Models in Drug Discovery

Autori: Tricot T, Verfaillie C, Kumar M
Pubblicato in: Cells, Numero january 27, 2022; 11: 442, 2022, ISSN 2078-2489
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cells11030442

Establishment of a human cell-based in vitro battery to assess developmental neurotoxicity hazard of chemicals

Autori: Blum J, Masjosthusmann S, Bartmann K, Bendt F, Dolde X, Dönmez A, Förster N, Holzer AK, Hübenthal U, Keßel HE, Kilic S, Klose J, Pahl M, Stürzl LC, Mangas I, Terron A, Crofton KM, Scholze M, Mosig A, Leist M, Fritsche E.
Pubblicato in: Chemosphere, Numero 31;311(Pt 2):137035, 2022, ISSN 1879-1298
Editore: Elsevier
DOI: 10.1016/j.chemosphere.2022.137035

Optimization of the TeraTox Assay for Preclinical Teratogenicity Assessment

Autori: Jaklin M, Zhang JD, Schäfer N, Clemann N, Barrow P, Küng E, Sach-Peltason L, McGinnis C, Leist M, Kustermann S.
Pubblicato in: Toxicological Sciences, Numero 188(1):17-33, 2022, ISSN 0029-3970
Editore: Oxford University Press
DOI: 10.1093/toxsci/kfac046

Circulating (Poly)phenol Metabolites: Neuroprotection in a 3D Cell Model of Parkinson’s Disease

Autori: Carecho R, Figueira I, Terrasso AP, Godinho-Pereira J, de Oliveira Sequeira C, Pereira SA, Milenkovic D, Leist M, Brito C, Nunes Dos Santos C.
Pubblicato in: Mol. Nutr. Food Res., Numero 29 December 2021, 2021, ISSN 1613-4125
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/mnfr.202100959

Mapping the dynamics of Nrf2 antioxidant and NFκB inflammatory responses by soft electrophilic chemicals in human liver cells defines the transition from adaptive to adverse responses

Autori: Ter Braak B, Klip JE, Wink S, Hiemstra S, Cooper SL, Middleton A, White A, van de Water B
Pubblicato in: Toxicology In Vitro, 2022, ISSN 1879-1298
Editore: Elsevier
DOI: 10.1016/j.tiv.2022.105419

Acute effects of the imidacloprid metabolite desnitro-imidacloprid on human nACh receptors relevant for neuronal signaling

Autori: Loser D, Grillberger K, Hinojosa MG, Blum J, Haufe Y, Danker T, Johansson Y, Möller C, Nicke A, Bennekou SH, Gardner I, Bauch C, Walker P, Forsby A, Ecker GF, Kraushaar U, Leist M.
Pubblicato in: Arch Toxicol, Numero 95(12).3695-3716, 2021, ISSN 2193-1801
Editore: Springer Science and Business Media Deutschland GmbH
DOI: 10.1007/s00204-021-03168-z

Transcriptomic-based evaluation of trichloroethylene glutathione and cysteine conjugates demonstrate phenotype-dependent stress responses in a panel of human in vitro models

Autori: Capinha L, Zhang Y, Holzer AK, Ückert AK, Zana M, Carta G, Murphy C, Baldovini J, Mazidi Z, Grillari J, Dinnyes A, van de Water B, Leist M, Commandeur JNM, Jennings P
Pubblicato in: Archives of Toxicology, 2023, ISSN 2193-1801
Editore: Springer Science and Business Media Deutschland GmbH
DOI: 10.1007/s00204-022-03436-6

Studying Metabolism by NMR-Based Metabolomics

Autori: Sofia Moco
Pubblicato in: Frontiers Molecular Biosciences, Numero 9:882487, 2022, 2022, ISSN 1663-9812
Editore: Frontiers Media S.A.
DOI: 10.3389/fmolb.2022.882487

Metabolic regulation by p53 prevents R-loop-associated genomic instability

Autori: Emanuele Panatta, Alessio Butera, Eleonora Mammarella, Consuelo Pitolli, Alessandro Mauriello, Marcel Leist, Richard A. Knight, Gerry Melino, Ivano Amelio
Pubblicato in: Cell Reports, Numero Volume 41, Numero 5, 1 November 2022, 2022, ISSN 2211-1247
Editore: Cell Press
DOI: 10.1016/j.celrep.2022.111568

High Accuracy Classification of Developmental Toxicants by In Vitro Tests of Human Neuroepithelial and Cardiomyoblast Differentiation

Autori: Seidel F, Cherianidou A, Kappenberg F, Marta M, Dreser N, Blum J, Waldmann T, Blüthgen N, Meisig J, Madjar K, Henry M, Rotshteyn T, Scholtz-Illigens A, Marchan R, Edlund K, Leist M, Rahnenführer J, Sachinidis A, Hengstler JG.
Pubblicato in: Cells, Numero 11(21):3404, 2022, ISSN 2078-2489
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cells11213404

Dynamic Metabolic and Transcriptional Responses of Proteasome-Inhibited Neurons

Autori: Suciu, I.; Delp, J.; Gutbier, S.; Ückert, A.-K.; Spreng, A.-S.; Eberhard, P.; Karreman, C.; Schreiber, F.; Madjar, K.; Rahnenführer, J.; Celardo, I.; Amelio, I.; Leist, M
Pubblicato in: Antioxidants, 2023, ISSN 2078-2489
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/antiox12010164

Distinct and Dynamic Transcriptome Adaptations of iPSC-Generated Astrocytes after Cytokine Stimulation

Autori: Spreng AS, Brüll M, Leisner H, Suciu I, Leist M.
Pubblicato in: Human Stem Cells in Disease Modelling, Drug Discovery and Regenerative Medicine, Numero 11(17):2644, 25 August 2022, 2022, ISSN 2078-2489
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cells11172644

Use of metabolic glycoengineering and pharmacological inhibitors to assess lipid and protein sialylation on cells.

Autori: Kranaster P, Blum J, Dold JEGA, Wittmann V, Leist M.
Pubblicato in: Neurochem, 2022, ISSN 1613-4125
Editore: John Wiley & Sons Ltd.
DOI: 10.1111/jnc.15737

Large-scale perfused tissues via synthetic 3D soft microfluidics

Autori: Grebenyuk S, Abdel Fattah AR, Kumar M, Toprakhisar B, Rustandi G, Vananroye A, Salmon I, Verfaillie C, Grillo M, Ranga A
Pubblicato in: Nat Commun, 2023, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-022-35619-1

On the Usefulness of Animals as a Model System (Part II): Considering Benefits within Distinct Use Domains

Autori: G. Pallocca, M. Leist
Pubblicato in: ALTEX- Alternatives to Animal Experimentation, Numero 39(3), 20 July 2022, 2022, ISSN 1868-8551
Editore: Springer Spektrum
DOI: 10.14573/altex.2207111

Synuclein Family Members Prevent Membrane Damage by Counteracting α-Synuclein Aggregation

Autori: Scheibe C, Karreman C, Schildknecht S, Leist M, Hauser K.
Pubblicato in: Biomolecules, Numero 11(8): 1067, Aug 2021, 2021, ISSN 2078-2489
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/biom11081067

Classification of Developmental Toxicants in a Human iPSC Transcriptomics-Based Test

Autori: Cherianidou A, Seidel F, Kappenberg F, Dreser N, Blum J, Waldmann T, Blüthgen N, Meisig J, Madjar K, Henry M, Rotshteyn T, Marchan R, Edlund K, Leist M, Rahnenführer J, Sachinidis A, Hengstler JG.
Pubblicato in: Chemical Research in Toxicology, 2022, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/acs.chemrestox.1c00392

Non-peer reviewed articles (2)

Guidance document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0

Autori: Pamies D, Leist M, Coecke S, Bowe G, Allen DG, Gstraunthaler G, Bal-Price A, Pistollato F, de Vries RBM, Hogberg HT, Hartung T, Stacey G.
Pubblicato in: ALTEX, Numero 39:30-70, 2022, ISSN 1868-8551
Editore: Springer Spektrum
DOI: 10.14573/altex.2111011

On the usefulness of animals as a model system (part I): Overview of criteria and focus on robustness

Autori: Pallocca G., Rovida C., Leist M
Pubblicato in: ALTEX, Numero 2022;39(2):347-353, 2022, ISSN 2193-1801
Editore: Springer Science and Business Media Deutschland GmbH
DOI: 10.14573/altex.2203291