Cel The testing of cosmetological (and pharmaceutical) preparations will have to be performed in a near future by procedures alternative to animal experiments to comply with the Sixth Amendment (93-35-EEC) to the EC Cosmetic Directive (976- 768-EEC). In vitro recombined skin using human cells represents an elegant procedure for this purpose. The presently available models suffer imperfections that will be addressed in the present project to aim at the preparation of an in-vitro test for skin irritancy. It will display meaningful endpoints, should prove reproducible by the use of chemically defined culture products and immortalised cell lines and be easy-to-use by introducing adequate markers of the irritant reaction. The programme will be a shared activity of six academic research units representing a large part of the scientific european potential in the domain and associated industrial laboratories experts in the use of reconstructed skin for irritant testing. The project will be developed in four partially overlapping steps addressing to defined problems. Step 1- improvement of the quality of the stratum corneum in-vitro, introduction of endothelial cells in the dermal compartment and determination of in-vivo related endpoints of irritancy. Step 2 - replacement of the human cell strains by immortalised human cell lines and determination of the conditions required to obtain near physiological functions and the above determined selected end-points of the irritant reaction. Step 3 introduction of the markers of the irritant reaction by infection or transfection of constructions made of a reporter gene driven by the promoter of a protein participating in the process in the different lines of cells (keratinocytes, fibroblasts and endothelial cells). Step 4 - preparation of a model suitable for prevalidation testing in collaboration with industrial partners. The benefits of the project is scientific by the european collaboration required to address to the specific know-how of the different types of cells included in the model, economical by helping the industry to comply to the EEC directives and ethical by a contribution to the faster suppression of testing in animals by an alternative procedure. Dziedzina nauki nauki przyrodniczenauki biologicznebiochemiabiocząsteczkibiałka Program(-y) FP4-BIOTECH 2 - Specific research, technological development and demonstration programme in the field of biotechnology, 1994-1998 Temat(-y) 070104 - Cell cultures for the development of in vitro tests Zaproszenie do składania wniosków Data not available System finansowania CSC - Cost-sharing contracts Koordynator Université de Liège Adres 4000 Liège Belgia Zobacz na mapie Wkład UE € 0,00 Uczestnicy (5) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko Institut National de la Santé et de la Recherche Médicale Francja Wkład UE € 0,00 Adres Avenue claude vellefaux 75475 Paris Zobacz na mapie Istituto di Ricerche Farmacologiche Mario Negri Włochy Wkład UE € 0,00 Adres Via eritrea 62 20157 Milano Zobacz na mapie Rijksuniversiteit Leiden Niderlandy Wkład UE € 0,00 Adres 10,rijnsburgerweg 2300 RC Leiden Zobacz na mapie Stiftung Deutsches Krebsforschungszentrum Niemcy Wkład UE € 0,00 Adres 280,im neuenheimer feld 69120 Heidelberg Zobacz na mapie Universität Köln Niemcy Wkład UE € 0,00 Adres 9,joseph stelzmann straße 50931 Köln Zobacz na mapie
