Obiettivo
* Development of candidate vaccines against parasitic filarial infections, in particular Onchocerca volvulus.
* To identify, isolate and express antigens that are specific to the larval stages of these parasites.
* To analyse of the immune responses of humans exposed to O.volvulus and animals infected with Acanthoche lonema viteae to these antigens.
* To assess the protective capacity of these antigens in a rodent filarial model system.
Expected Outcome
* The identification of novel candidate vaccine antigens for onchocerciasis.
* The identification of the type of immune responses that are protectiveagainst filarial infections in humans and animal models.
* Production of quantities of L3 and L4 larval stages of the parasites Onchocerca volvulus and Acanthocheilonema viteae.
* Identification and characterisation of antigens that are specific to the larval stages of the parasite by protein analysis and differential display PCR.
* Cloning and Expression of stage specific antigens from cDNA libraries of each larval stage.
* Characterisation of the cellular and humoral responses to the larval antigens of putatively immune humans exposed to onchocerciasis transmisson.
* Evaluation of the protective capacity of the recombinant larval antigens in a rodent filarial model system.
Campo scientifico
Argomento(i)
Invito a presentare proposte
Data not availableMeccanismo di finanziamento
CSC - Cost-sharing contractsCoordinatore
M5 4WT Salford
Regno Unito