Technologies of the Future for Low-Cost Enzymes for Environment-Friendly Products

Informazioni relative al progetto

FuturEnzyme

ID dell’accordo di sovvenzione: 101000327

DOI 10.3030/101000327

Data della firma CE 22 Aprile 2021

Data di avvio 1 Giugno 2021

Data di completamento 31 Maggio 2025

Finanziato da

• SOCIETAL CHALLENGES - Food security, sustainable agriculture and forestry, marine, maritime and inland water research, and the bioeconomy

Costo totale € 5 995 035,13

Contributo UE € 5 995 035,13

Coordinato da

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain

Risultati finali

Websites, patent fillings, videos etc. (3)

Visual identity guidelines

This deliverable will consist in a series of visual elements to create distinction and a strong visual identity for the FuturEnzyme project as well as template for presentations posters and reports

FuturEnzyme website

This deliverable will consist in an open website to assure communication towards the outside world that will be maintained and regularly updated throughout the project The website will serve as the main interface for distributing project information and results as elaborated in all WPs

FuturEnzyme project leaflet and brochure

This deliverable will consist in a series of leaflet and brochure materials for disseminating and communicating Project objectives and outcomes for consumers policymakers This material will be available through the website

Pubblicazioni

Peer reviewed articles (38)

Improving bioprocess conditions for the production of prodigiosin using a marine Serratia rubidaea strain

Autori: Ricardo F. S. Pereira, Carla C. C. R. de Carvalho
Pubblicato in: Marine Drugs, Numero 22(4), 2024, Pagina/e 142, ISSN 1660-3397
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/md22040142

Metagenomic mining for esterases in the microbial community of Los Rueldos acid mine drainage formation

Autori: Paula Vidal, Mónica Martínez-Martínez, Laura Fernandez-Lopez, Sergi Roda, Celia Méndez-García, Olga V. Golyshina, Víctor Guallar, Ana I. Peláez, Manuel Ferrer
Pubblicato in: Frontiers in Microbiology, Numero Volume 13, 2022, Pagina/e 868839, ISSN 1664-302X
Editore: Frontiers Media
DOI: 10.3389/fmicb.2022.868839

An archaeal lid-containing feruloyl esterase degrades polyethylene terephthalate

Autori: Perez-Garcia, Pablo; Chow, Jennifer; Costanzi, Elisa; Gurschke, Marno; Dittrich, Jonas; Dierkes, Robert F.; Molitor, Rebecka; Applegate, Violetta; Feuerriegel, Golo; Tete, Prince; Danso, Dominik; Thies, Stephan; Schumacher, Julia; Pfleger, Christopher; Jaeger, Karl-Erich; Gohlke, Holger; Smits, Sander H. J.; Schmitz, Ruth A.; Streit, Wolfgang R.
Pubblicato in: Communications Chemistry, Numero 6, 2023, Pagina/e 193, ISSN 2399-3669
Editore: Nature Portfolio
DOI: 10.1038/s42004-023-00998-z

A Choice Experiment Model for Sustainable Consumer Goods: A Systematic Literature Review and Workflow Design

Autori: Saija, Maria Elena; Daniotti, Sara; Bosco, Diego; Re, Ilaria
Pubblicato in: A Choice Experiment Model for Sustainable Consumer Goods: A Systematic Literature Review and Workflow Design, Numero 15, 2023, Pagina/e 13183, ISSN 2071-1050
Editore: MDPI Open Access Publishing
DOI: 10.3390/su151713183

Process Development for Benzyl Alcohol Production by Whole-Cell Biocatalysis in Stirred and Packed Bed Reactors

Autori: Carlos Rodrigues; Carla De Carvalho
Pubblicato in: Microorganisms, Numero Volume 10; Numero 5, 2022, Pagina/e 966, ISSN 2076-2607
Editore: MDPI
DOI: 10.3390/microorganisms10050966

A novel metagenome-derived viral RNA polymerase and its application in a cell-free expression system for metagenome screening

Autori: Yuchen Han, Birhanu M. Kinfu, Fabian Blombach, Gwenny Cackett, Hongli Zhang, Pablo Pérez‑García, Ines Krohn, Jesper Salomon, Volkan Besirlioglu, Tayebeh Mirzaeigarakani, Ulrich Schwaneberg, Jennifer Chow, Finn Werner, Wolfgang R. Streit
Pubblicato in: Scientific Reports, Numero Volume 12, 2022, Pagina/e 17882, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-022-22383-x

Design and Characterization of In-One Protease-Esterase PluriZyme

Autori: Fernandez-Lopez, Laura; Roda, Sergi; Gonzalez-Alfonso, José L; Plou, Francisco J; Guallar, Víctor; Ferrer, Manuel
Pubblicato in: International Journal of Molecular Sciences, Numero Volume 23, Numero 21, 2022, Pagina/e 13337, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms232113337

Plasmonic photothermal activation of an organosilica shielded cold-adapted lipase coimmobilised with gold nanoparticles on silica particles

Autori: Giunta, Carolina I; Nazemi, Seyed Amirabbas; Olesinska, Magdalena; Shahgaldian, Patrick
Pubblicato in: Nanoscale Advances, Numero 5, 2023, Pagina/e 81-87, ISSN 2516-0230
Editore: Royal Society of Chemistry
DOI: 10.1039/d2na00605g

Enhancing the Hydrolytic Activity of a Lipase towards Larger Triglycerides through Lid Domain Engineering

Autori: Fernandez-Lopez, Laura; Roda, Sergi; Robles-Martín, A; Muñoz-Tafalla, Rubén; Almendral, David; Ferrer, Manuel; Guallar, Víctor
Pubblicato in: International Journal of Molecular Sciences, Numero 24, 2023, Pagina/e 18, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms241813768

Sub-micro- and nano-sized polyethylene terephthalate deconstruction with engineered protein nanopores

Autori: Robles-Martín, Ana; Amigot-Sánchez, Rafael; Fernandez-Lopez, Laura; Gonzalez-Alfonso, Jose L; Roda, Sergi; Alcolea-Rodriguez, Víctor; Heras-Márquez, Diego; Almendral, David; Coscolín, Cristina; Plou, Francisco J; Portela, Raquel; Bañares, Miguel A; Martínez-del-Pozo, Álvaro; García-Linares, Sara; Ferrer, Manuel; Guallar, Víctor
Pubblicato in: Nature Catalysis, Numero 6, 2023, Pagina/e 174–1185, ISSN 2520-1158
Editore: Nature Portfolio
DOI: 10.1038/s41929-023-01048-6

Natranaeroarchaeum sulfidigenes gen. nov., sp. nov., carbohydrate-utilizing sulfur-respiring haloarchaeon from hypersaline soda lakes, a member of a new family Natronoarchaeaceae fam. nov. in the order Halobacteriales

Autori: Dimitry Y. Sorokin, Michail Yakimov, Enzo Messina, Alexander Y. Merkel, Michel Koenen, Nicole J. Bale, Jaap S. Sinninghe Damsté
Pubblicato in: ystematic and Applied Microbiology, Numero Volume 45, 2022, Pagina/e 126356, ISSN 0723-2020
Editore: Elsevier BV
DOI: 10.1016/j.syapm.2022.126356

Increased Thermostability of an Engineered Flavin-Containing Monooxygenase to Remediate Trimethylamine in Fish Protein Hydrolysates

Autori: Goris, Marianne; Cea-Rama, Isabel; Puntervoll, Pål; Ree, Rasmus; Almendral, David; Sanz-Aparicio, Julia; Ferrer, Manuel; Bjerga, Gro Elin Kjæreng
Pubblicato in: Applied and Environmental Microbiology, Numero 2, 2023, ISSN 0099-2240
Editore: American Society for Microbiology
DOI: 10.1128/aem.00390-23

Anaerobic carboxydotrophy in sulfur-respiring haloarchaea from hypersaline lakes

Autori: Dimitry Y. Sorokin, Alexander Y. Merkel, Enzo Messina, Claudia Tugui, Martin Pabst, Peter N. Golyshin & Michail M. Yakimov 
Pubblicato in: The ISME Journal, Numero Volume 16, 2022, Pagina/e 1534–1546, ISSN 1751-7362
Editore: Nature Publishing Group
DOI: 10.1038/s41396-022-01206-x

Exploiting cyclodextrins as artificial chaperones to enhance enzyme protection through supramolecular engineering

Autori: Foroutan Kalourazi, Ali; Amirabbas Nazemi, Seyed; Unniram Parambi, Ajmal Roshan; Muñoz-Tafalla, Ruben; Vidal, Paula; Shahangian, S. Shirin; Guallar, Víctor; Ferrer, Manuel; Shahgaldian, Patrick
Pubblicato in: Nanoscale, Numero 16, 2024, Pagina/e 5123-5129, ISSN 2040-3372
Editore: The Royal Society of Chemistry
DOI: 10.1039/D3NR06044F

Isolation and Characterization of a Serratia rubidaea from a Shallow Water Hydrothermal Vent

Autori: Pereira, Ricardo F. S.; Ferreira, Maria J.; Oliveira, M. Conceição; Serra, Maria C.; de Carvalho, Carla C. C. R.
Pubblicato in: Marine Drugs, Numero 21, 2023, Pagina/e 599, ISSN 1660-3397
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/md21120599

Halopseudomonas species: Cultivation and molecular genetic tools

Autori: Kruse, Luzie; Loeschcke, Anita; de Witt, Jan; Wierckx, Nick; Jaeger, Karl-Erich; Thies, Stephan
Pubblicato in: Microbial Biotechnology, Numero 17, 2024, Pagina/e e14369, ISSN 1751-7915
Editore: Wiley
DOI: 10.1111/1751-7915.14369

Calm and Frenzy: marine obligate hydrocarbonoclastic bacteria sustain ocean wellness

Autori: Michail M. Yakimov; Rafael Bargiela; Peter N. Golyshin
Pubblicato in: Current Opinion in Biotechnology, Numero Volume 73, 2022, Pagina/e 337-345, ISSN 0958-1669
Editore: Elsevier BV
DOI: 10.1016/j.copbio.2021.09.015

Crystal structure of a family VIII β-lactamase fold hydrolase reveals the molecular mechanism for its broad substrate scope

Autori: Isabel Cea-Rama, Cristina Coscolín, Jose L. Gonzalez-Alfonso, Jog Raj, Marko Vasiljević, Francisco J. Plou, Manuel Ferrer, Julia Sanz-Aparicio
Pubblicato in: FEBS Journal, Numero Volume 289, Numero 21, 2022, Pagina/e 6714-6730, ISSN 1742-464X
Editore: Blackwell Publishing Inc.
DOI: 10.1111/febs.16554

Transforming an esterase into an enantioselective catecholase through bioconjugation of a versatile metal-chelating inhibitor

Autori: Fernandez-Lopez, Laura; Cea-Rama, Isabel; Alvarez-Malmagro, Julia; Ressmann, Anna K; Gonzalez-Alfinso, Jose Luis; Coscolín, Cristina; Shahgaldian, Patrick; Plou, Francisco J; Modregger, Jan; Pita, Marcos; Sanz-Aparicio, Julia; Ferrer, Manuel
Pubblicato in: Chemical Communications, Numero 1, 2023, ISSN 1359-7345
Editore: Royal Society of Chemistry
DOI: 10.1039/D3CC01946B

Thermophilic carboxylesterases from hydrothermal vents of the volcanic island of Ischia active on synthetic and biobased polymers and mycotoxins

Autori: Distaso, Marco; Chernikova, Tatyana; Bargiela, Rafael; Coscolín, Cristina; Stogios, Peter; Gonzalez-Alfonso, Jose L; Lemak, Sofia; Khusnutdinova, Anna; Plou, Francisco J; Evdokimova, Elena; Savchenko, Alexei; Lunev, Evgenii A; Yakimov, Michail M; Golyshina, Olga; Ferrer, Manuel; Yakunin, Alexander F; Golyshin, Peter N
Pubblicato in: Applied and Environmental Microbiology, 2023, ISSN 0099-2240
Editore: American Society for Microbiology
DOI: 10.1101/2022.09.17.508236

AsiteDesign: a Semirational Algorithm for an Automated EnzymeDesign

Autori: Roda, Sergi; Terholsen, Henrik; Heike Meyer, Jule Ruth; Cañellas-Solé, Albert; Guallar, Víctor; Bornscheuer, Uwe; Kazemi, Masoud
Pubblicato in: The Journal of Physical Chemistry B, Numero 127, 2023, Pagina/e 2661−2670, ISSN 1520-6106
Editore: American Chemical Society
DOI: 10.1021/acs.jpcb.2c07091

The metagenome-derived esterase PET40 is highlypromiscuous and hydrolyses polyethyleneterephthalate (PET)

Autori: Zhang, Hongli; Dierkes, Robert F.; Perez-Garcia, Pablo; Costanzi, Elisa; Dittrich, Jonas; Cea, Pablo A.; Gurschke, Marno; Applegate, Violetta; Partus, Kristina; Schmeisser, Christel; Pfleger, Christopher; Gohlke, Holger; Smits, Sander H. J.; Jennifer Chow; Wolfgang R. Streit
Pubblicato in: The FEBS Journal, 2023, ISSN 1742-464X
Editore: Blackwell Publishing Inc.
DOI: 10.1111/febs.16924

EP-Pred: A Machine Learning Tool for Bioprospecting Promiscuous Ester Hydrolases

Autori: Xiang, R; Fernandez-Lopez, Laura; Robles-Martín, Ana; Ferrer, Manuel; Guallar, Víctor
Pubblicato in: Biomolecules, Numero Volume 12, issue 10, 2022, Pagina/e 1529, ISSN 2218-273X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/biom12101529

Interplay of intracellular and trans-cellular DNA methylation in natural archaeal consortia

Autori: Reva, Oleg N.; La Cono, Violetta; Crisafi, Francesca; Smedile, Francesco; Mudaliyar, Manasi; Ghosal, Debnath; Giuliano, Laura; Krupovic, Mart; Yakimov, Michail M.
Pubblicato in: Environmental Microbiology Reports, Numero 16, 2024, Pagina/e e13258, ISSN 1758-2229
Editore: Wiley-Blackwell
DOI: 10.1111/1758-2229.13258

A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions

Autori: Sergi Roda, Laura Fernandez-Lopez, Marius Benedens, Alexander Bollinger, Stephan Thies, Julia Schumacher, Cristina Coscolín, Masoud Kazemi, Gerard Santiago, Christoph G. W. Gertzen, Jose L. Gonzalez-Alfonso, Francisco J. Plou, Karl-Erich Jaeger, Sander H. J. Smits, Manuel Ferrer, Víctor Guallar
Pubblicato in: Angewandte Chemie International Edition, Numero Volume 61, Numero 37, 2022, Pagina/e e202207344, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202207344

Cultivation of a vampire: ‘Candidatus Absconditicoccus praedator’

Autori: Michail M. Yakimov; Alexander Y. Merkel; Vasil A. Gaisin; Martin Pilhofer; Enzo Messina; John E. Hallsworth; Alexandra A. Klyukina; Ekaterina N. Tikhonova; Vladimir M. Gorlenko
Pubblicato in: Environmental Microbiology, Numero Volume 24, Numero 1, 2021, Pagina/e 30-49, ISSN 1462-2920
Editore: Wiley-Blackwell
DOI: 10.1111/1462-2920.15823

Enzymes for consumer products to achieve climate neutrality

Autori: Molina-Espeja, Patricia; Sanz-Aparicio, Julia; Golyshin, Peter N; Robles-Martín, Ana; Guallar, Victor; Beltrametti, Fabrizio; Müller, Markus; Yakimov, Mikhail; Modregger, Jan; van Logchem, Moniec; Corvini, Philippe; Shahgaldian, Patrick; Degering, Christian; Wieland, Susanne; Timm, Anne; de Carvalho, Carla CCR; Ilaria, Re; Daniotti, Sara; Thies, Stephan; Jaeger, Karl-Erich; Chow, Jennifer; Strei
Pubblicato in: Oxford Open Climate Change, Numero 3, 2023, Pagina/e kgad003, ISSN 2634-4068
Editore: Oxford University Press
DOI: 10.1093/oxfclm/kgad003

An archaeal lid-containing feruloyl-esterase degrades polyethylene terephthalate (PET)

Autori: Perez-Garcia, Pablo; Chow, Jennifer; Costanzi, Elisa; Gurschke, Marno; Dittrich, Jonas; Dierkes, Robert F.; Molitor, Rebecka; Applegate, Violetta; Feuerriegel, Golo; Tete, Prince; Danso, Dominik; Thies, Stephan; Schumacher, Julia; Pfleger, Christopher; Jaeger, Karl-Erich; Gohlke, Holger; Smits, Sander H. J.; Schmitz, Ruth A.; Streit, Wolfgang R.
Pubblicato in: Communications chemistry, Numero 6, 2023, Pagina/e 193, ISSN 2399-3669
Editore: Nature Portfolio
DOI: 10.21203/rs.3.rs-3196380/v1

The mobility of the cap domain is essential for the substrate promiscuity of a family IV esterase from sorghum rhizosphere microbiome

Autori: Distaso, Marco; Cea-Rama, Isabel; Coscolín, Cristina; Chernikova, Tatyana; Tran, Hai; Ferrer, Manuel; Sanz-Aparicio, Julia; Golyshin, Peter
Pubblicato in: Applied and Environmental Microbiology, 2023, ISSN 0099-2240
Editore: American Society for Microbiology
DOI: 10.1128/aem.01807-22

Nanohaloarchaea as beneficiaries of xylan degradation by haloarchaea

Autori: La Cono, Violetta; Messina, Enzo; Reva, Olga; Smedile, Francesco; La Spada, Gina; Crisafi, Francesca; Marturano, Laura; Miguez, Noa; Ferrer, Manuel; Selivanova, Elena A; Golyshina, Olga V; Golyshin, Peter N; Rohde, Manfred; Krupovic, Mart; Merkel, Alexander Y; Sorokin, Dimitry Y; Hallsworth, John E; Yakimov, Michail M
Pubblicato in: Microbial Biotechnology, 2023, ISSN 1751-7915
Editore: Wiley-Blackwell
DOI: 10.1111/1751-7915.14272

Halapricum desulfuricans sp. nov., carbohydrate-utilizing, sulfur-respiring haloarchaea from hypersaline lakes

Autori: Dimitry Y. Sorokin, Michail M. Yakimov, Enzo Messina, Alexander Y. Merkel, Michel Koenen, Nicole J. Bale, Jaap S. Sinninghe D
Pubblicato in: Systematic and Applied Microbiology, Numero 44, 2021, Pagina/e 126249, ISSN 0723-2020
Editore: Elsevier BV
DOI: 10.1016/j.syapm.2021.126249

Marine Bioprospecting, Biocatalysis and Process Development

Autori: Rodrigues, Carlos J.C.; de Carvalho, Carla C.C.R.
Pubblicato in: Microorganisms, Numero Volume 10, issue 10, 2022, Pagina/e 1965, ISSN 2076-2607
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/microorganisms10101965

Functional diversity of nanohaloarchaea within xylan-degrading consortia

Autori: Oleg, Reva; Enzo, Messina; Violetta, La Cono; Francesca, Crisafi; Francesco, Smedile; Gina, La Spada; Laura, Marturano; Elena A, Selivanova; Manfred, Rohde; Mart, Krupovic; Michail M, Yakimov
Pubblicato in: Frontiers in Microbiology, Numero 14, 2023, Pagina/e 1182464, ISSN 1664-302X
Editore: Frontiers Media
DOI: 10.3389/fmicb.2023.1182464

Nanobiocatalysts with inbuilt cofactor recycling for oxidoreductase catalysis in organic solvents

Autori: Sahlin, Jenny; Wu, Congyu; Buscemi, Andrea; Schärer, Claude; Nazemi, Seyed Amirabbas; Rejaul, S. K.; Herrera-Reinoza, Nataly; Jung, Thomas A.; Shahgaldian, Patrick
Pubblicato in: Nanoscale Advances, Numero 5, 2023, Pagina/e 5036-5044, ISSN 2516-0230
Editore: The Royal Society of Chemistry
DOI: 10.1039/D3NA00413A

Enzyme adaptation to habitat thermal legacy shapes the thermal plasticity of marine microbiomes

Autori: Marasco, Ramona; Fusi, Marco; Coscolín, Cristina; Barozzi, Alan; Almendral, David; Bargiela, Rafael; Gohlke neé Nutschel, Christina; Pfleger, Christopher; Dittrich, Jonas; Gohlke, Holger; Matesaz, Ruth; Sanchez-Carrillo, Sergio; Mapelli, Francesca; Chernikova, Tatynana N; Golyshin, Peter N; Ferrer, Manuel; Daffonchio, Daniele
Pubblicato in: Nature Communications, Numero 14, 2023, Pagina/e 1045, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-36610-0

Behind the Scenes of PluriZyme Designs

Autori: Robles-Martín, Ana; Roda, Sergi; Muñoz-Tafalla, Rubén; Guallar, Víctor
Pubblicato in: Eng, Numero 5(1), 2024, Pagina/e 91-103, ISSN 2673-4117
Editore: MDPI
DOI: 10.3390/eng5010006

Harnessing extremophilic carboxylesterases for applications in polyester depolymerisation and plastic waste recycling

Autori: Williams, Gwion B; Ma, Hairong; Khusnutdinova, Anna N.; Yakunin, Alexander F.; Golyshin, Peter N
Pubblicato in: Essays in Biochemistry, Numero 67, 2023, Pagina/e 715-729, ISSN 0071-1365
Editore: Portland Press, Ltd.
DOI: 10.1042/EBC20220255

Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma

Autori: Carlos J. C. Rodrigues, Carla C. C. R. de Carvalho
Pubblicato in: Frontiers in Bioengineering and Biotechnology, Numero Volume 10, 2022, Pagina/e 964589, ISSN 2296-4185
Editore: Frontiers Media S.A.
DOI: 10.3389/fbioe.2022.964589

Other (2)

Fantastic enzymes: where and how to find them

Autori: Ferrer, Manuel; Molina-Espeja, Patricia; Daniotti, Sara; Müller, Markus
Pubblicato in: The FEBS Network, 2022
Editore: Wiley-Blackwell Publishing Ltd
DOI: 10.5281/zenodo.7260738

Se buscan enzimas, razón: el medio ambiente

Autori: Manuel Ferrer, Cristina Coscolín Galán, Patricia Molina Espeja
Pubblicato in: The Conversation España, 2021, ISSN 2201-5639
Editore: The Conversation
DOI: 10.5281/zenodo.5566131

Non-peer reviewed articles (2)

The first archaeal PET-degrading enzyme belongs to the feruloyl-esterase family

Autori: Perez-Garcia, Pablo; Chow, Jennifer; Costanzi, Elisa; Gurschke, Marno F; Dittrich, Jonas; Dierkes, Robert F; Applegate, Violetta; Feuerriegel, Golo; Tete, Prince; Danso, Dominik; Schumacher, Julia; Pfleger, Christopher; Gohlke, Holger; Smits, Sander HJ; Schmitz, Ruth A; Streit, Wolfgang
Pubblicato in: bioRvix, 2022, ISSN 2692-8205
Editore: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.10.14.512230

El uso de enzimas para conseguir productos de consumo diario más sostenibles

Autori: Ferrer, Manuel; Molina, Patricia; Peñalver, Carolina
Pubblicato in: Revista Ambienta, Numero 128 (Innovation), 2021, ISSN 2605-4787
Editore: Gobierno de España, Ministerio para la Transición Ecológica y el Reto Demográfico
DOI: 10.5281/zenodo.5384608