Technologies of the Future for Low-Cost Enzymes for Environment-Friendly Products

Rezultaty

This deliverable will consist in a series of visual elements to create distinction and a strong visual identity for the FuturEnzyme project as well as template for presentations posters and reports

FuturEnzyme website

This deliverable will consist in an open website to assure communication towards the outside world that will be maintained and regularly updated throughout the project The website will serve as the main interface for distributing project information and results as elaborated in all WPs

FuturEnzyme project leaflet and brochure

This deliverable will consist in a series of leaflet and brochure materials for disseminating and communicating Project objectives and outcomes for consumers policymakers This material will be available through the website

Publikacje

Improving bioprocess conditions for the production of prodigiosin using a marine Serratia rubidaea strain

Autorzy: Ricardo F. S. Pereira, Carla C. C. R. de Carvalho
Opublikowane w: Marine Drugs, Numer 22(4), 2024, Strona(/y) 142, ISSN 1660-3397
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/md22040142

Metagenomic mining for esterases in the microbial community of Los Rueldos acid mine drainage formation

Autorzy: Paula Vidal, Mónica Martínez-Martínez, Laura Fernandez-Lopez, Sergi Roda, Celia Méndez-García, Olga V. Golyshina, Víctor Guallar, Ana I. Peláez, Manuel Ferrer
Opublikowane w: Frontiers in Microbiology, Numer Volume 13, 2022, Strona(/y) 868839, ISSN 1664-302X
Wydawca: Frontiers Media
DOI: 10.3389/fmicb.2022.868839

An archaeal lid-containing feruloyl esterase degrades polyethylene terephthalate

Autorzy: Perez-Garcia, Pablo; Chow, Jennifer; Costanzi, Elisa; Gurschke, Marno; Dittrich, Jonas; Dierkes, Robert F.; Molitor, Rebecka; Applegate, Violetta; Feuerriegel, Golo; Tete, Prince; Danso, Dominik; Thies, Stephan; Schumacher, Julia; Pfleger, Christopher; Jaeger, Karl-Erich; Gohlke, Holger; Smits, Sander H. J.; Schmitz, Ruth A.; Streit, Wolfgang R.
Opublikowane w: Communications Chemistry, Numer 6, 2023, Strona(/y) 193, ISSN 2399-3669
Wydawca: Nature Portfolio
DOI: 10.1038/s42004-023-00998-z

A Choice Experiment Model for Sustainable Consumer Goods: A Systematic Literature Review and Workflow Design

Autorzy: Saija, Maria Elena; Daniotti, Sara; Bosco, Diego; Re, Ilaria
Opublikowane w: A Choice Experiment Model for Sustainable Consumer Goods: A Systematic Literature Review and Workflow Design, Numer 15, 2023, Strona(/y) 13183, ISSN 2071-1050
Wydawca: MDPI Open Access Publishing
DOI: 10.3390/su151713183

Process Development for Benzyl Alcohol Production by Whole-Cell Biocatalysis in Stirred and Packed Bed Reactors

Autorzy: Carlos Rodrigues; Carla De Carvalho
Opublikowane w: Microorganisms, Numer Volume 10; Numer 5, 2022, Strona(/y) 966, ISSN 2076-2607
Wydawca: MDPI
DOI: 10.3390/microorganisms10050966

A novel metagenome-derived viral RNA polymerase and its application in a cell-free expression system for metagenome screening

Autorzy: Yuchen Han, Birhanu M. Kinfu, Fabian Blombach, Gwenny Cackett, Hongli Zhang, Pablo Pérez‑García, Ines Krohn, Jesper Salomon, Volkan Besirlioglu, Tayebeh Mirzaeigarakani, Ulrich Schwaneberg, Jennifer Chow, Finn Werner, Wolfgang R. Streit
Opublikowane w: Scientific Reports, Numer Volume 12, 2022, Strona(/y) 17882, ISSN 2045-2322
Wydawca: Nature Publishing Group
DOI: 10.1038/s41598-022-22383-x

Design and Characterization of In-One Protease-Esterase PluriZyme

Autorzy: Fernandez-Lopez, Laura; Roda, Sergi; Gonzalez-Alfonso, José L; Plou, Francisco J; Guallar, Víctor; Ferrer, Manuel
Opublikowane w: International Journal of Molecular Sciences, Numer Volume 23, Numer 21, 2022, Strona(/y) 13337, ISSN 1422-0067
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms232113337

Plasmonic photothermal activation of an organosilica shielded cold-adapted lipase coimmobilised with gold nanoparticles on silica particles

Autorzy: Giunta, Carolina I; Nazemi, Seyed Amirabbas; Olesinska, Magdalena; Shahgaldian, Patrick
Opublikowane w: Nanoscale Advances, Numer 5, 2023, Strona(/y) 81-87, ISSN 2516-0230
Wydawca: Royal Society of Chemistry
DOI: 10.1039/d2na00605g

Enhancing the Hydrolytic Activity of a Lipase towards Larger Triglycerides through Lid Domain Engineering

Autorzy: Fernandez-Lopez, Laura; Roda, Sergi; Robles-Martín, A; Muñoz-Tafalla, Rubén; Almendral, David; Ferrer, Manuel; Guallar, Víctor
Opublikowane w: International Journal of Molecular Sciences, Numer 24, 2023, Strona(/y) 18, ISSN 1422-0067
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms241813768

Sub-micro- and nano-sized polyethylene terephthalate deconstruction with engineered protein nanopores

Autorzy: Robles-Martín, Ana; Amigot-Sánchez, Rafael; Fernandez-Lopez, Laura; Gonzalez-Alfonso, Jose L; Roda, Sergi; Alcolea-Rodriguez, Víctor; Heras-Márquez, Diego; Almendral, David; Coscolín, Cristina; Plou, Francisco J; Portela, Raquel; Bañares, Miguel A; Martínez-del-Pozo, Álvaro; García-Linares, Sara; Ferrer, Manuel; Guallar, Víctor
Opublikowane w: Nature Catalysis, Numer 6, 2023, Strona(/y) 174–1185, ISSN 2520-1158
Wydawca: Nature Portfolio
DOI: 10.1038/s41929-023-01048-6

Natranaeroarchaeum sulfidigenes gen. nov., sp. nov., carbohydrate-utilizing sulfur-respiring haloarchaeon from hypersaline soda lakes, a member of a new family Natronoarchaeaceae fam. nov. in the order Halobacteriales

Autorzy: Dimitry Y. Sorokin, Michail Yakimov, Enzo Messina, Alexander Y. Merkel, Michel Koenen, Nicole J. Bale, Jaap S. Sinninghe Damsté
Opublikowane w: ystematic and Applied Microbiology, Numer Volume 45, 2022, Strona(/y) 126356, ISSN 0723-2020
Wydawca: Elsevier BV
DOI: 10.1016/j.syapm.2022.126356

Increased Thermostability of an Engineered Flavin-Containing Monooxygenase to Remediate Trimethylamine in Fish Protein Hydrolysates

Autorzy: Goris, Marianne; Cea-Rama, Isabel; Puntervoll, Pål; Ree, Rasmus; Almendral, David; Sanz-Aparicio, Julia; Ferrer, Manuel; Bjerga, Gro Elin Kjæreng
Opublikowane w: Applied and Environmental Microbiology, Numer 2, 2023, ISSN 0099-2240
Wydawca: American Society for Microbiology
DOI: 10.1128/aem.00390-23

Anaerobic carboxydotrophy in sulfur-respiring haloarchaea from hypersaline lakes

Autorzy: Dimitry Y. Sorokin, Alexander Y. Merkel, Enzo Messina, Claudia Tugui, Martin Pabst, Peter N. Golyshin & Michail M. Yakimov
Opublikowane w: The ISME Journal, Numer Volume 16, 2022, Strona(/y) 1534–1546, ISSN 1751-7362
Wydawca: Nature Publishing Group
DOI: 10.1038/s41396-022-01206-x

Exploiting cyclodextrins as artificial chaperones to enhance enzyme protection through supramolecular engineering

Autorzy: Foroutan Kalourazi, Ali; Amirabbas Nazemi, Seyed; Unniram Parambi, Ajmal Roshan; Muñoz-Tafalla, Ruben; Vidal, Paula; Shahangian, S. Shirin; Guallar, Víctor; Ferrer, Manuel; Shahgaldian, Patrick
Opublikowane w: Nanoscale, Numer 16, 2024, Strona(/y) 5123-5129, ISSN 2040-3372
Wydawca: The Royal Society of Chemistry
DOI: 10.1039/D3NR06044F

Isolation and Characterization of a Serratia rubidaea from a Shallow Water Hydrothermal Vent

Autorzy: Pereira, Ricardo F. S.; Ferreira, Maria J.; Oliveira, M. Conceição; Serra, Maria C.; de Carvalho, Carla C. C. R.
Opublikowane w: Marine Drugs, Numer 21, 2023, Strona(/y) 599, ISSN 1660-3397
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/md21120599

Halopseudomonas species: Cultivation and molecular genetic tools

Autorzy: Kruse, Luzie; Loeschcke, Anita; de Witt, Jan; Wierckx, Nick; Jaeger, Karl-Erich; Thies, Stephan
Opublikowane w: Microbial Biotechnology, Numer 17, 2024, Strona(/y) e14369, ISSN 1751-7915
Wydawca: Wiley
DOI: 10.1111/1751-7915.14369

Calm and Frenzy: marine obligate hydrocarbonoclastic bacteria sustain ocean wellness

Autorzy: Michail M. Yakimov; Rafael Bargiela; Peter N. Golyshin
Opublikowane w: Current Opinion in Biotechnology, Numer Volume 73, 2022, Strona(/y) 337-345, ISSN 0958-1669
Wydawca: Elsevier BV
DOI: 10.1016/j.copbio.2021.09.015

Crystal structure of a family VIII β-lactamase fold hydrolase reveals the molecular mechanism for its broad substrate scope

Autorzy: Isabel Cea-Rama, Cristina Coscolín, Jose L. Gonzalez-Alfonso, Jog Raj, Marko Vasiljević, Francisco J. Plou, Manuel Ferrer, Julia Sanz-Aparicio
Opublikowane w: FEBS Journal, Numer Volume 289, Numer 21, 2022, Strona(/y) 6714-6730, ISSN 1742-464X
Wydawca: Blackwell Publishing Inc.
DOI: 10.1111/febs.16554

Transforming an esterase into an enantioselective catecholase through bioconjugation of a versatile metal-chelating inhibitor

Autorzy: Fernandez-Lopez, Laura; Cea-Rama, Isabel; Alvarez-Malmagro, Julia; Ressmann, Anna K; Gonzalez-Alfinso, Jose Luis; Coscolín, Cristina; Shahgaldian, Patrick; Plou, Francisco J; Modregger, Jan; Pita, Marcos; Sanz-Aparicio, Julia; Ferrer, Manuel
Opublikowane w: Chemical Communications, Numer 1, 2023, ISSN 1359-7345
Wydawca: Royal Society of Chemistry
DOI: 10.1039/D3CC01946B

Thermophilic carboxylesterases from hydrothermal vents of the volcanic island of Ischia active on synthetic and biobased polymers and mycotoxins

Autorzy: Distaso, Marco; Chernikova, Tatyana; Bargiela, Rafael; Coscolín, Cristina; Stogios, Peter; Gonzalez-Alfonso, Jose L; Lemak, Sofia; Khusnutdinova, Anna; Plou, Francisco J; Evdokimova, Elena; Savchenko, Alexei; Lunev, Evgenii A; Yakimov, Michail M; Golyshina, Olga; Ferrer, Manuel; Yakunin, Alexander F; Golyshin, Peter N
Opublikowane w: Applied and Environmental Microbiology, 2023, ISSN 0099-2240
Wydawca: American Society for Microbiology
DOI: 10.1101/2022.09.17.508236

AsiteDesign: a Semirational Algorithm for an Automated EnzymeDesign

Autorzy: Roda, Sergi; Terholsen, Henrik; Heike Meyer, Jule Ruth; Cañellas-Solé, Albert; Guallar, Víctor; Bornscheuer, Uwe; Kazemi, Masoud
Opublikowane w: The Journal of Physical Chemistry B, Numer 127, 2023, Strona(/y) 2661−2670, ISSN 1520-6106
Wydawca: American Chemical Society
DOI: 10.1021/acs.jpcb.2c07091

The metagenome-derived esterase PET40 is highlypromiscuous and hydrolyses polyethyleneterephthalate (PET)

Autorzy: Zhang, Hongli; Dierkes, Robert F.; Perez-Garcia, Pablo; Costanzi, Elisa; Dittrich, Jonas; Cea, Pablo A.; Gurschke, Marno; Applegate, Violetta; Partus, Kristina; Schmeisser, Christel; Pfleger, Christopher; Gohlke, Holger; Smits, Sander H. J.; Jennifer Chow; Wolfgang R. Streit
Opublikowane w: The FEBS Journal, 2023, ISSN 1742-464X
Wydawca: Blackwell Publishing Inc.
DOI: 10.1111/febs.16924

EP-Pred: A Machine Learning Tool for Bioprospecting Promiscuous Ester Hydrolases

Autorzy: Xiang, R; Fernandez-Lopez, Laura; Robles-Martín, Ana; Ferrer, Manuel; Guallar, Víctor
Opublikowane w: Biomolecules, Numer Volume 12, issue 10, 2022, Strona(/y) 1529, ISSN 2218-273X
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/biom12101529

Interplay of intracellular and trans-cellular DNA methylation in natural archaeal consortia

Autorzy: Reva, Oleg N.; La Cono, Violetta; Crisafi, Francesca; Smedile, Francesco; Mudaliyar, Manasi; Ghosal, Debnath; Giuliano, Laura; Krupovic, Mart; Yakimov, Michail M.
Opublikowane w: Environmental Microbiology Reports, Numer 16, 2024, Strona(/y) e13258, ISSN 1758-2229
Wydawca: Wiley-Blackwell
DOI: 10.1111/1758-2229.13258

A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions

Autorzy: Sergi Roda, Laura Fernandez-Lopez, Marius Benedens, Alexander Bollinger, Stephan Thies, Julia Schumacher, Cristina Coscolín, Masoud Kazemi, Gerard Santiago, Christoph G. W. Gertzen, Jose L. Gonzalez-Alfonso, Francisco J. Plou, Karl-Erich Jaeger, Sander H. J. Smits, Manuel Ferrer, Víctor Guallar
Opublikowane w: Angewandte Chemie International Edition, Numer Volume 61, Numer 37, 2022, Strona(/y) e202207344, ISSN 1433-7851
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202207344

Cultivation of a vampire: ‘Candidatus Absconditicoccus praedator’

Autorzy: Michail M. Yakimov; Alexander Y. Merkel; Vasil A. Gaisin; Martin Pilhofer; Enzo Messina; John E. Hallsworth; Alexandra A. Klyukina; Ekaterina N. Tikhonova; Vladimir M. Gorlenko
Opublikowane w: Environmental Microbiology, Numer Volume 24, Numer 1, 2021, Strona(/y) 30-49, ISSN 1462-2920
Wydawca: Wiley-Blackwell
DOI: 10.1111/1462-2920.15823

Enzymes for consumer products to achieve climate neutrality

Autorzy: Molina-Espeja, Patricia; Sanz-Aparicio, Julia; Golyshin, Peter N; Robles-Martín, Ana; Guallar, Victor; Beltrametti, Fabrizio; Müller, Markus; Yakimov, Mikhail; Modregger, Jan; van Logchem, Moniec; Corvini, Philippe; Shahgaldian, Patrick; Degering, Christian; Wieland, Susanne; Timm, Anne; de Carvalho, Carla CCR; Ilaria, Re; Daniotti, Sara; Thies, Stephan; Jaeger, Karl-Erich; Chow, Jennifer; Strei
Opublikowane w: Oxford Open Climate Change, Numer 3, 2023, Strona(/y) kgad003, ISSN 2634-4068
Wydawca: Oxford University Press
DOI: 10.1093/oxfclm/kgad003

An archaeal lid-containing feruloyl-esterase degrades polyethylene terephthalate (PET)

Autorzy: Perez-Garcia, Pablo; Chow, Jennifer; Costanzi, Elisa; Gurschke, Marno; Dittrich, Jonas; Dierkes, Robert F.; Molitor, Rebecka; Applegate, Violetta; Feuerriegel, Golo; Tete, Prince; Danso, Dominik; Thies, Stephan; Schumacher, Julia; Pfleger, Christopher; Jaeger, Karl-Erich; Gohlke, Holger; Smits, Sander H. J.; Schmitz, Ruth A.; Streit, Wolfgang R.
Opublikowane w: Communications chemistry, Numer 6, 2023, Strona(/y) 193, ISSN 2399-3669
Wydawca: Nature Portfolio
DOI: 10.21203/rs.3.rs-3196380/v1

The mobility of the cap domain is essential for the substrate promiscuity of a family IV esterase from sorghum rhizosphere microbiome

Autorzy: Distaso, Marco; Cea-Rama, Isabel; Coscolín, Cristina; Chernikova, Tatyana; Tran, Hai; Ferrer, Manuel; Sanz-Aparicio, Julia; Golyshin, Peter
Opublikowane w: Applied and Environmental Microbiology, 2023, ISSN 0099-2240
Wydawca: American Society for Microbiology
DOI: 10.1128/aem.01807-22

Nanohaloarchaea as beneficiaries of xylan degradation by haloarchaea

Autorzy: La Cono, Violetta; Messina, Enzo; Reva, Olga; Smedile, Francesco; La Spada, Gina; Crisafi, Francesca; Marturano, Laura; Miguez, Noa; Ferrer, Manuel; Selivanova, Elena A; Golyshina, Olga V; Golyshin, Peter N; Rohde, Manfred; Krupovic, Mart; Merkel, Alexander Y; Sorokin, Dimitry Y; Hallsworth, John E; Yakimov, Michail M
Opublikowane w: Microbial Biotechnology, 2023, ISSN 1751-7915
Wydawca: Wiley-Blackwell
DOI: 10.1111/1751-7915.14272

Halapricum desulfuricans sp. nov., carbohydrate-utilizing, sulfur-respiring haloarchaea from hypersaline lakes

Autorzy: Dimitry Y. Sorokin, Michail M. Yakimov, Enzo Messina, Alexander Y. Merkel, Michel Koenen, Nicole J. Bale, Jaap S. Sinninghe D
Opublikowane w: Systematic and Applied Microbiology, Numer 44, 2021, Strona(/y) 126249, ISSN 0723-2020
Wydawca: Elsevier BV
DOI: 10.1016/j.syapm.2021.126249

Marine Bioprospecting, Biocatalysis and Process Development

Autorzy: Rodrigues, Carlos J.C.; de Carvalho, Carla C.C.R.
Opublikowane w: Microorganisms, Numer Volume 10, issue 10, 2022, Strona(/y) 1965, ISSN 2076-2607
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/microorganisms10101965

Functional diversity of nanohaloarchaea within xylan-degrading consortia

Autorzy: Oleg, Reva; Enzo, Messina; Violetta, La Cono; Francesca, Crisafi; Francesco, Smedile; Gina, La Spada; Laura, Marturano; Elena A, Selivanova; Manfred, Rohde; Mart, Krupovic; Michail M, Yakimov
Opublikowane w: Frontiers in Microbiology, Numer 14, 2023, Strona(/y) 1182464, ISSN 1664-302X
Wydawca: Frontiers Media
DOI: 10.3389/fmicb.2023.1182464

Nanobiocatalysts with inbuilt cofactor recycling for oxidoreductase catalysis in organic solvents

Autorzy: Sahlin, Jenny; Wu, Congyu; Buscemi, Andrea; Schärer, Claude; Nazemi, Seyed Amirabbas; Rejaul, S. K.; Herrera-Reinoza, Nataly; Jung, Thomas A.; Shahgaldian, Patrick
Opublikowane w: Nanoscale Advances, Numer 5, 2023, Strona(/y) 5036-5044, ISSN 2516-0230
Wydawca: The Royal Society of Chemistry
DOI: 10.1039/D3NA00413A

Enzyme adaptation to habitat thermal legacy shapes the thermal plasticity of marine microbiomes

Autorzy: Marasco, Ramona; Fusi, Marco; Coscolín, Cristina; Barozzi, Alan; Almendral, David; Bargiela, Rafael; Gohlke neé Nutschel, Christina; Pfleger, Christopher; Dittrich, Jonas; Gohlke, Holger; Matesaz, Ruth; Sanchez-Carrillo, Sergio; Mapelli, Francesca; Chernikova, Tatynana N; Golyshin, Peter N; Ferrer, Manuel; Daffonchio, Daniele
Opublikowane w: Nature Communications, Numer 14, 2023, Strona(/y) 1045, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-023-36610-0

Behind the Scenes of PluriZyme Designs

Autorzy: Robles-Martín, Ana; Roda, Sergi; Muñoz-Tafalla, Rubén; Guallar, Víctor
Opublikowane w: Eng, Numer 5(1), 2024, Strona(/y) 91-103, ISSN 2673-4117
Wydawca: MDPI
DOI: 10.3390/eng5010006

Harnessing extremophilic carboxylesterases for applications in polyester depolymerisation and plastic waste recycling

Autorzy: Williams, Gwion B; Ma, Hairong; Khusnutdinova, Anna N.; Yakunin, Alexander F.; Golyshin, Peter N
Opublikowane w: Essays in Biochemistry, Numer 67, 2023, Strona(/y) 715-729, ISSN 0071-1365
Wydawca: Portland Press, Ltd.
DOI: 10.1042/EBC20220255

Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma

Autorzy: Carlos J. C. Rodrigues, Carla C. C. R. de Carvalho
Opublikowane w: Frontiers in Bioengineering and Biotechnology, Numer Volume 10, 2022, Strona(/y) 964589, ISSN 2296-4185
Wydawca: Frontiers Media S.A.
DOI: 10.3389/fbioe.2022.964589

Fantastic enzymes: where and how to find them

Autorzy: Ferrer, Manuel; Molina-Espeja, Patricia; Daniotti, Sara; Müller, Markus
Opublikowane w: The FEBS Network, 2022
Wydawca: Wiley-Blackwell Publishing Ltd
DOI: 10.5281/zenodo.7260738

Se buscan enzimas, razón: el medio ambiente

Autorzy: Manuel Ferrer, Cristina Coscolín Galán, Patricia Molina Espeja
Opublikowane w: The Conversation España, 2021, ISSN 2201-5639
Wydawca: The Conversation
DOI: 10.5281/zenodo.5566131

The first archaeal PET-degrading enzyme belongs to the feruloyl-esterase family

Autorzy: Perez-Garcia, Pablo; Chow, Jennifer; Costanzi, Elisa; Gurschke, Marno F; Dittrich, Jonas; Dierkes, Robert F; Applegate, Violetta; Feuerriegel, Golo; Tete, Prince; Danso, Dominik; Schumacher, Julia; Pfleger, Christopher; Gohlke, Holger; Smits, Sander HJ; Schmitz, Ruth A; Streit, Wolfgang
Opublikowane w: bioRvix, 2022, ISSN 2692-8205
Wydawca: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.10.14.512230

El uso de enzimas para conseguir productos de consumo diario más sostenibles

Autorzy: Ferrer, Manuel; Molina, Patricia; Peñalver, Carolina
Opublikowane w: Revista Ambienta, Numer 128 (Innovation), 2021, ISSN 2605-4787
Wydawca: Gobierno de España, Ministerio para la Transición Ecológica y el Reto Demográfico
DOI: 10.5281/zenodo.5384608

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