Together with the roadmap a communication channel with the EBA was found to share insights and build a strong network. Publications where created in collaboration between partners. Microbial samples were collected and analyzed from 45 AD plants across Europe. Microbiome analyses revealed that all reactors shared a common "core" microbiome. This group of prokaryotes was thoroughly characterized using various omic techniques, physicochemical parameters and correlated with microbiome profiles. Hundreds of microorganisms were isolated, enabling their use in subsequent WPs. Eenrichment cultures of previously unculturable microorganisms, such as members of the Darwinibacteriales order were successfully established. The results of the first artificially evolved microorganism with improved DIET, for bioaugmentation strategies in AD and the results about the enrichment of microaerophilic, acidifying microbiomes and their suitability for nitrogen elimination were finished. Key achievements include the development of evolved methanogens and electroactive strains, microaerophilic microbiome enrichment, and electrochemical adaptation strategies. All planned experiments to test bioaugmentation and transplantation include: lab- (<1L), medium- (1–70 L), and large-scale (70–5,000 L) for validation of different microbial single strains, combinations of them, and/or full microbiomes (up to 30% improvement in biogas production). Environmental and social LCA was conducted including impact assessment results and thorough interpretation of these results. Regarding the S-LCA, the focus was on the industrial scale cases. A comparative analysis between conventional and bioaugmentation-enhanced biogas production was provided for all cases. The Techno-Economic Assessment (TEA) offered a detailed financial outlook, including net profit, Free Cash Flow, and investment indicators over ten years. Sensitivity analyses evaluated the financial robustness under varying conditions, identifying key cost drivers. A comprehensive exploitation strategy was developed around 12 Key Exploitable Results (KERs), prioritized by TRL and market potential. A full market analysis highlighted the European biogas sector's structure, policy influences, and biomethane competitiveness. A business plan for a bioaugmentation kit projected a 39% CH4 yield increase, supported by a €1.9M roadmap and B2B model. An IPR strategy was successfully implemented, covering patents, trade secrets, and FTO protocols. A comprehensive dissemination plan was executed, including the launch of a visual identity, website, blog, podcast, and five active social media channels. Scientific dissemination was supported through publications and events, while outreach included a school biodigester activity and e-learning platform. Over 500.000 social media impressions, more than 30.000 website visits, and strong engagement metrics across all platforms reflect the success of these efforts.
