MOlecular-Scale Biophysics Research Infrastructure

Informazioni relative al progetto

MOSBRI

ID dell’accordo di sovvenzione: 101004806

DOI

10.3030/101004806

Data della firma CE 16 Marzo 2021

Data di avvio 1 Luglio 2021

Data di completamento 31 Ottobre 2025

Finanziato da

• EXCELLENT SCIENCE - Research Infrastructures

Costo totale

€ 4 996 058,14

Contributo UE

€ 4 996 058,00

Coordinato da

INSTITUT PASTEUR
France

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Websites, patent fillings, videos etc. (4)

Dissemination of novel methodologies via website

Dissemination of novel methodologies via websiteDeliverable to be submitted in M12 M24 and M48

Operational proposal system based on ARIA cloud service
Driving licence specifications and test materials at Operator level
MOSBRI website

Documents, reports (5)

Report on joint events organized with ARBRE and EBSA

Report on joint events organized with ARBRE and EBSADeliverable to be submitted in M18 M30 and M42

Analysis of the mapping of the needs for data standards/formats

Analysis of the mapping of the needs for data standardsformats

Established reference calibration samples
Analysis of the mapping of the needs for repository/database

Analysis of the mapping of the needs for repositorydatabase

Report on joint events organized with INSTRUCT

Report on joint events organized with INSTRUCTDeliverable to be submitted in M18 M30 and M42

Other (1)

Setting-up of a TNA moderator panel and of a TNA reviewer panel

Pubblicazioni

Peer reviewed articles (34)

Interaction of a Histidine-Rich Antimicrobial Saliva Peptide with Model Cell Membranes: The Role of Histidines

Autori: Skog, A. E., Corucci, G., Tully, M. D., Fragneto, G., Gerelli, Y., Skepö, M.
Pubblicato in: Langmuir, Numero 39, 2023, Pagina/e 7694, ISSN 1520-5827
Editore: ACS Publications
DOI: 10.1021/acs.langmuir.3c00498

Temoneira-1 beta-lactamase is not a metalloenzyme, but its native metal ion binding sites allow for purification by immobilized metal ion affinity chromatography

Autori: Nafaee, Z.H., Hunyadi-Gulyas, E., Gyurcsik, B.
Pubblicato in: Protein Expression and Purification, 2023, ISSN 1046-5928
Editore: Academic Press
DOI: 10.1016/j.pep.2022.106169

Structure and conformational variability of the HER2-trastuzumab-pertuzumab complex

Autori: Ruedas, R., Vuillemot, R., Tubiana, T., Winter, J. M., Pieri, L., Arteni, A.-A., Samson, C., Jonic, S., Mathieu, M., Bressanelli, S.
Pubblicato in: Journal of Structural Biology, 2024, ISSN 1047-8477
Editore: Academic Press
DOI: 10.1016/j.jsb.2024.108095

The phosphodiesterase RmcA contributes to the adaptation of Pseudomonas putida to l-arginine

Autori: Scribani-Rossi C, Molina-Henares MA, Angeli S, Cutruzzolà F, Paiardini A, Espinosa-Urgel M, Rinaldo S.
Pubblicato in: FEMS Microbiology Letters, Numero Volume 370, 2023, ISSN 1574-6968
Editore: Oxford university press
DOI: 10.1093/femsle/fnad077

Electrochemical Kinetics Support a Second Coordination Sphere Mechanism in Metal-Based Formate Dehydrogenase

Autori: Marta Meneghello, Alexandre Uzel, Marianne Broc, Rita R. Manuel, Axel Magalon, Christophe Léger, Inês A. C. Pereira, Anne Walburger, Vincent Fourmond
Pubblicato in: Angewandte Chemie - International edition, Numero 134, e202299999, 2022, ISSN 1521-3773
Editore: Wiley-VCH GmbH
DOI: 10.1002/anie.202212224

Visualizing Molecular Dynamics by High-Speed Atomic Force Microscopy

Autori: van Ewijk, C., Maity, S., Roos, W.H.
Pubblicato in: Single Molecule Analysis . Methods in Molecular Biology, 2023, ISSN 1064-3745
Editore: Humana Press, Inc.
DOI: 10.1007/978-1-0716-3377-9_17

Interactions and Insertion of Escherichia coli Hfq into Outer Membrane Vesicles as Revealed by Infrared and Orientated Circular Dichroism Spectroscopies

Autori: Turbant, F., Waeytens, J., Blache, A., Esnouf, E., Raussens, V., Wegrzyn, G., Achouak, W., Wien, F., Arluison, V.
Pubblicato in: International Journal of Molecular Sciences, Numero 24(14), 11424, 2023, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms241411424

Despite the odds: formation of the SARS-CoV-2 methylation complex

Autori: Matsuda, A., Plewka, J., Rawski, M., Mourão, A., Zajko, W., Siebenmorgen, T., Kresik, L., Lis, K., Jones, A. N., Pachota, M., Karim, A., Hartman, K., Nirwal, S., Sonani, R., Chykunova,Y., Minia, I., Mak, P., Landthaler, M., Nowotny, M., Dubin, G., Sattler, M., Suder, P., Popowicz, G. M., Pyrć, K., Czarna, A.
Pubblicato in: Nucleic Acids Research, 2024, ISSN 1362-4962
Editore: Oxford university press
DOI: 10.1093/nar/gkae165

Lateral membrane organization as target of an antimicrobial peptidomimetic compound

Autori: Melcrova, A., Maity, S., Melcr, J., de Kok, N.A.W., Gabler, M., van der Eyden, J., Stensen, W., Svendsen, J.S.M., Driessen, A.J.M., Marrink, S.J., Roos, W.H.
Pubblicato in: Nature Communications, Numero 14(1) ar4038, 2023, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-39726-5

Influence of the enzymatic treatment and pH on the interfacial and emulsifying properties of sunflower and olive protein hydrolysates

Autori: Pérez-Gálvez, R., Maldonado-Valderrama, J., Jones, N. C., Hoffmann, S. V., Guadix, E., García-Moreno, P. J.
Pubblicato in: Food Hydrocolloids, Numero 154 ar110135, 2024, ISSN 0268-005X
Editore: Elsevier BV
DOI: 10.1016/j.foodhyd.2024.110135

13th EBSA congress, July 24–28, 2021, Vienna, Austria

Autori: 13th EBSA congress
Pubblicato in: European Biophysics Journal, Numero 50/S1, 2021, Pagina/e 1-226, ISSN 0175-7571
Editore: Springer Verlag
DOI: 10.1007/s00249-021-01558-w

ATR-FTIR Biosensors for Antibody Detection and Analysis

Autori: Suys, O., Derenne, A., Goormaghtigh, E.
Pubblicato in: International Journal of Molecular Sciences, Numero 23(19), 2022, Pagina/e 11895, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms231911895

Biochemical characterization of L-asparaginase isoforms from Rhizobium etli—the boosting effect of zinc

Autori: Sliwiak, J., Worsztynowicz, P., Pokrywka, K., Loch, J. I., Grzechowiak, M., Jaskolski, M.
Pubblicato in: Frontiers in Chemistry, 2024, ISSN 2296-2646
Editore: Frontiers Media S.A
DOI: 10.3389/fchem.2024.1373312

Protein Structural Denaturation Evaluated by MCR-ALS of Protein Microarray FTIR Spectra

Autori: De Meutter, J., Goormaghtigh, E.
Pubblicato in: Analytical Chemistry, Numero 93(40), 2021, Pagina/e 13441-13449, ISSN 1520-6882
Editore: American Chemical Society publications
DOI: 10.1021/acs.analchem.1c01416

Molecular insights into RmcA-mediated c-di-GMP consumption: Linking redox potential to biofilm morphogenesis in Pseudomonas aeruginosa

Autori: Scribani Rossi, C., Eckartt, K., Scarchilli, E., Angeli, S., Price-Whelan, A., Di Matteo, A., Chevreuil, M., Raynal, B., Arcovito, A., Giacon, N., Fiorentino, F., Rotili, D.,
Pubblicato in: Microbiological Research, Numero Volume 277, 127498, 2023, ISSN 1618-0623
Editore: Elsevier
DOI: 10.1016/j.micres.2023.127498

Synthesis of 3,4-Disubstituted Pyrroline Nitroxides Containing Diphenylphosphane or Diphenylphosphane Oxide Substituents

Autori: Balázsi, A., Bognár, B., Jeko, J., Kálai, T.
Pubblicato in: SynOpen, 2024, ISSN 2509-9396
Editore: Thieme
DOI: 10.1055/a-2264-8302

Raynals, an online tool for the analysis of dynamic light scattering.

Autori: Burastero, O., Draper-Barr, G., Raynal, B., Chevreuil, M., England, P., Garcia-Alai, M.
Pubblicato in: Acta Crystallographica Section D, Numero 79, 2023, Pagina/e p673–683, ISSN 2059-7983
Editore: IUCr journals
DOI: 10.1107/s2059798323004862

Exploring the Metabolic Response of Pseudomonas putida to L-arginine

Autori: Scribani-Rossi, C., Molina-Henares, M. A., Espinosa-Urgel, M., Rinaldo, S.
Pubblicato in: Advances in Experimental Medicine and Biology, 2024, ISSN 0065-2598
Editore: Kluwer Academic/Plenum Publishers
DOI: 10.1007/5584_2024_797

Limited high-throughput screening compatibility of the phenuivirus cap-binding domain

Autori: Scherf, J., Vogel, D., Gul, S., Reinshagen, J., Gribbon, P., Rosenthal, M.
Pubblicato in: Scientific Reports, Numero 13, 2023, Pagina/e 22820, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-023-50158-5

Guidelines for the Simulations of Nitroxide X-Band cw EPR Spectra from Site-Directed Spin Labeling Experiments Using SimLabel

Autori: Etienne, E., Pierro, A., Tamburrini, K. C., Bonucci, A., Mileo, E., Martinho, M., Belle, V.
Pubblicato in: Molecules, Numero 28, 2023, Pagina/e 1348, ISSN 1420-3049
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules28031348

Effect of Cholesterol on Biomimetic Membrane Curvature and Coronavirus Fusion Peptide Encapsulation

Autori: Milogrodzka, I., Pham, D.T.N., Sama, G.R., Samadian, H., Zhai, J.L., de Campo, L., Kirby, N.M., Scott, T.F., Holl, M.M.B., van’t Hag, L.
Pubblicato in: ACS Nano, Numero 17 (9), 2023, Pagina/e 8598-8612, ISSN 1936-0851
Editore: American Chemical Society
DOI: 10.1021/acsnano.3c01095

In-cell investigation of the conformational landscape of the GTPase UreG by SDSL-EPR

Autori: Pierro, A., Tamburrini, K.C., Leguenno, H., Gerbaud, G., Etienne, E., Guigliarelli, B., Belle, V., Zambelli, B., Mileo, E.
Pubblicato in: iScience, Numero 26(10), 2023, Pagina/e 107855, ISSN 2589-0042
Editore: cell press
DOI: 10.1016/j.isci.2023.107855

Conformational analysis of membrane-proximal segments of GDAP1 in a lipidic environment using synchrotron radiation suggests a mode of assembly at the mitochondrial outer membrane

Autori: Sutinen, A., Jones, N. C., Hoffmann, S. V., Ruskamo, S., & Kursula, P.
Pubblicato in: Biophysical Chemistry, Numero 303, 107113, 2023, ISSN 0301-4622
Editore: Elsevier BV
DOI: 10.1016/j.bpc.2023.107113

The Amyloid Assembly of the Bacterial Hfq Is Lipid-Driven and Lipid-Specific

Autori: Turbant, F., Machiels, Q., Waeytens, J., Wien, F., Arluison, V.
Pubblicato in: International Journal of Molecular Sciences, 2024, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms25031434

Structural insights into the semiquinone form of human Cytochrome P450 reductase by DEER distance measurements between a native flavin and a spin labelled non-canonical amino acid

Autori: Bizet, M., Byrne, D., Biaso, F., Gerbaud, G., Etienne, E., Briola, G., Guigliarelli, B., Urban, P., Dorlet, P., Kalai, T., Truan, G., Martinho, M.
Pubblicato in: Chemistry – A European Journal,, Numero 30 e202304307, 2024, ISSN 0947-6539
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/chem.202304307

Pea and soy protein isolate fractal gels: the role of protein composition, structure and solubility on their gelation behaviour

Autori: Tiong, A. Y. J., Crawford, S., Jones, N. C., McKinley, G., Batchelor, W., Hag, L. van ’t
Pubblicato in: Food Structure, 2024, ISSN 2213-3291
Editore: Elsevier BV
DOI: 10.1016/j.foostr.2024.100374

Molecular dynamics simulations of the adsorption of an intrinsically disordered protein: Force field and water model evaluation in comparison with experiments

Autori: Mona Koder Hamid, Linda K. Månsson, Viktoriia Meklesh, Per Persson and Marie Skepö
Pubblicato in: Frontiers in Molecular Biosciences, Numero 26 October 2022, 2022, ISSN 2296-889X
Editore: Frontiers Media S.A.
DOI: 10.3389/fmolb.2022.958175

Conserved intramolecular networks in GDAP1 are closely connected to CMT-linked mutations and protein stability

Autori: Sutinen, A., Paffenholz, D., Nguyen, G.T.T., Ruskamo, S., Torda, A.E., Kursula, P.
Pubblicato in: plos One, Numero 18(4) e0284532, 2023, ISSN 1932-6203
Editore: Public Library of Science
DOI: 10.1371/journal.pone.0284532

Chiroptical properties of membrane glycerophospholipids and their chiral backbones

Autori: Bocková, J., Garcia, A. D., Jones, N. C., Hoffmann, S. V., Meinert, C.
Pubblicato in: Chirality, Numero 36(3), e23654, 2024, ISSN 0899-0042
Editore: John Wiley & Sons Inc.
DOI: 10.1002/chir.23654

Fluorescence labeling methods influence the aggregation process of α-syn in vitro differently

Autori: Jadavi, S., Dante, S., Civiero, L., Sandre, M., Bubacco, L., Tosatto, L., Bianchini, P., Canale, C., Diaspro, A.
Pubblicato in: Nanoscale, Numero 15, 2023, Pagina/e 8270, ISSN 2040-3372
Editore: royal society of chemistry
DOI: 10.1039/d2nr05487f

The human TRPA1 intrinsic cold and heat sensitivity involves separate channel structures beyond the N-ARD domain

Autori: Lavanya Moparthi, Viktor Sinica, Vamsi K. Moparthi, Mohamed Kreir, Thibaut Vignane, Milos R. Filipovic, Viktorie Vlachova & Peter M. Zygmunt
Pubblicato in: Nature communications, Numero 13:6113, 17 October 2022, 2022, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-022-33876-8

Antimicrobial Activity of an Fmoc-Plantaricin 149 Derivative Peptide against Multidrug-Resistant Bacteria.

Autori: Righetto, G.M.; Lopes, J.L.d.S.; Bispo, P.J.M.; André, C.; Souza, J.M.; Andricopulo, A.D.; Beltramini, L.M.; Camargo, I.L.B.d.C.
Pubblicato in: Anitbiotics, Numero 12, 2023, Pagina/e 391, ISSN 2079-6382
Editore: MDPI
DOI: 10.3390/antibiotics12020391

Advantages of a synchrotron light source for fluorescence-detected linear dichroism

Autori: Jones, N. C., Rodger, A., Hoffmann, S. V.
Pubblicato in: Chirality, Numero 36(4) e23667, 2024, ISSN 0899-0042
Editore: John Wiley & Sons Inc.
DOI: 10.1002/chir.23667

An allosteric redox switch involved in oxygen protection in a CO2 reductase

Autori: Oliveira, A.R., Mota, C., Vilela-Alves, G., Manuel, R.R., Pedrosa, N., Fourmond, V., Klymanska, K., Léger, C., Guigliarelli, B., Romão, M.J., Pereira, I.A.C.
Pubblicato in: Nature Chemical Biology, Numero 20, 111-119, 2024, ISSN 1465-7392
Editore: Nature Publishing Group
DOI: 10.1038/s41589-023-01484-2

Conference proceedings (8)

Thermodynamic landscape of the redox-centres in the electron-confurcating [FeFe]-Hydrogenase (HydABC) of Thermotoga maritima

Autori: Kilian Zuchan, Wolfgang Nitschke, Frauke Baymann, Nina Breuer, James Birell
Pubblicato in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Numero Volume 1863, Supplement, 29, 2022, Pagina/e 55, ISSN 0005-2728
Editore: Elsevier
DOI: 10.1016/j.bbabio.2022.148757

MOSBRI- A New European Research Infrastructure for Molecular Biophysics

Autori: Pierre Dorlet, Guillaume Gerbaud, Emilien Etienne, Stéphane Grimaldi, Bruno Guigliarelli, Valérie Belle.
Pubblicato in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Numero Volume 1863, Supplement 29, 2022, Pagina/e 28, ISSN 0005-2728
Editore: Elsevier
DOI: 10.1016/j.bbabio.2022.148684

NAD(H)-induced flavosemiquinone stabilization in the electron-confurcating [FeFe]-Hydrogenase (HydABC) of Thermotoga maritima

Autori: Kilian Zuchan, Wolfgang Nitschke, Frauke Baymann, Nina Breuer, James Birell
Pubblicato in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Numero Volume 1863, Supplement, 29, 2022, Pagina/e 55, ISSN 0005-2728
Editore: Elsevier
DOI: 10.1016/j.bbabio.2022.148758

TNA: Apply for access to laboratories of excellence in molecular scale biophysics research infrastructure (MOSBRI)

Autori: Hoffmann, S., Jones, N., Raynal, B., Von Castelmur, E., Derbyshire, D., Sunnerhagen, M.
Pubblicato in: Acta Crystallographica A – Foundation and Advances, Numero 78 E350-E350, 2022, ISBN 9780190080112
Editore: John Wiley & Sons
DOI: 10.1107/s2053273322093810

4th EBSA congress, July 31 – August 4, 2023, Stockholm, Sweden. Abstract: The MOlecular-Scale Biophysics Research Infrastructure (MOSBRI): a transnational initiative to structure and consolidate the European molecular biophysics community

Autori: England, P.
Pubblicato in: European Biophysics Journal with Biophysical Letters, Numero 52 S178-S178, 2023
Editore: Springer Nature
DOI: 10.1007/s00249-023-01668-7

Advanced in-house X-ray diffraction and scattering instruments as a service for structural biology

Autori: Stransky, J., Pavlicek, J., Dohnalek, J.
Pubblicato in: 2023
Editore: John Wiley & Sons
DOI: 10.1107/s2053273322093111

EPR-MRS site: EPR spectroscopy for the study of biomolecules

Autori: Pierre Dorlet, Guillaume Gerbaud, Emilien Etienne, Stéphane Grimaldi, Bruno Guigliarelli, Valérie Belle
Pubblicato in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Numero Volume 1863, Supplement 29, 2022, Pagina/e 29, ISSN 0005-2728
Editore: Elsevier
DOI: 10.1016/j.bbabio.2022.148685

Spectroscopic insights into the mechanism of anamox hydrazine synthase

Autori: Wouter Versantvoort, Rainer Hienerwader, Christina Ferousi, Catherine Berthomieu, Laura van Niftrik, Frauke Baymann
Pubblicato in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Numero Volume 1863, Supplement, 29, 2022, Pagina/e 76, ISSN 0005-2728
Editore: Elsevier
DOI: 10.1016/j.bbabio.2022.148818