The project advanced root exudate research in A. thaliana by developing a novel hydroponic system for exudate collection. It identified 373 chemical compounds in exudates from 105 genotypes, revealing 25 heritable compounds. This suggests genetic influence on exudate composition, potentially aiding in kin recognition. GWA analysis uncovered 26 genes related to metabolism, defense, and nutrient transport, emphasizing a genetic basis for exudate diversity. Contrary to expectations, ecological and geographic factors showed minimal influence on exudate variation. These findings pave the way for future plant-plant interaction studies and could enhance agricultural breeding programs for crop resilience. The project's blend of molecular biology, ecology, and analytical chemistry offers significant insights into plant root exudates, contributing to both ecological understanding and agricultural applications.
Researcher Training and Transfer-of-Knowledge
The fellow developed proficiency in GCMS and LCMS at Marianne Glasius's lab and in-vitro cultivation at Simona Radutoiu's lab at Aarhus University. These skills were pivotal for root exudate extraction and analysis. Enhanced knowledge in GCMS analysis at MG's lab, crucial for future chemical analysis of root exudates. Adiitionally, abilities in genomic data management and interpretation were strengthened through collaborations with Xavier Picó and Thomas Bataillon. To integrate interdisciplinary insights, the fellow organized a collaborative meeting in April 2023, focusing on comprehensive data analysis and advancing knowledge in the field.
Publications
• Subrahmaniam H.J. Lind Salomonsen. C, Radutoiu. S, Ehlers, B.K. Glasius, M. (2023). Unraveling the secrets of plant roots: Simplified method for large scale root exudate sampling and analysis in Arabidopsis thaliana. Open Research Europe, 3:12.
• Subrahmaniam H.J. Picó, X., Bataillon, T., Glasius, M., Radutoiu, S., Ehlers, B.K. Root exudates composition within and among four genetic clusters of Arabidopsis thaliana from the Iberian Peninsula (in prep)
Dissemination of results and outreach
Conference Participation and Presentations:
Delivered oral presentations at Nordic Society Oikos and DANEMO conferences (2022-2023).
Participated as a finalist in MSCA-Falling Walls.
Conducted seminars at Aarhus University in Chemistry, Molecular Biology, and Eco-Science departments, promoting academic exchange.
Research Supervision and Mentoring:
Supervised and mentored five M.Sc. and B.Sc. projects in the Chemistry department, fostering the development of emerging scientists.
Guided prospective STEMM graduates through Lotus STEMM, focusing on research opportunities in Europe, including MSCA fellowships.
Outreach and Support for Educators and Researchers Researchers
• Serves as chairperson and active participant in Marie Curie Alumni Association (MCAA) policy initiatives for over two years and a guest advisor for the Plant Ecology collection in Open Research Europe
• Committee member at the Dept. of Ecoscience for Early Career Researchers.
• Presented at over 10 seminars on diverse topics like plant sciences, science policy, mental health, women in science, and climate
• Authored blogs about the MSCA fellowship, guiding prospective students.
Recommendations and reports
Key Recommendatons by the Marie Curie Alumni Association (MCAA) on the Marie Skłodowska-Curie Actions (MSCA) Programme. Marie Curie Alumni Association. Contributors:
Book Chapter
Authored a chapter titled "STEM Women's Career Challenges and Possible Solutions" in "Empowering Women in STEM: Personal Stories and Career Journeys from Around the World" (CRC Press, 2022), highlighting challenges faced by women in STEM and proposing actionable solutions.
