Periodic Reporting for period 1 - PANORAMIX (Providing risk assessments of complex real-life mixtures for the protection of Europe?s citizens and the environment)
Okres sprawozdawczy: 2021-11-01 do 2023-04-30
The objectives of PANORAMIX are:
• to characterize chemical mixtures and to what extent they are transferred from the environment to humans via food and water, and from the mother to her foetus
• to establish a strategy for screening of real-life complex mixtures of chemicals extracted from human blood, food and environmental samples based on whole-mixture assessment in bioassays
• to identify mixture effects and their drivers in humans using integrated chemical profiling together with effects obtained in bioassays (i.e. effect-directed analysis)
• to evaluate which human blood levels of chemical mixtures might be of concern for reproductive and neuropsychological health in children
• to develop a pragmatic web-based interface to enable component-based mixture risk estimation
To determine the chemical drivers of the mixture effects, we need an initial filter to focus on chemicals of potential concern. Therefore, we are combining bioassay effects of extracts with chemical identification (so-called effect-directed analysis) with the goal of identifying chemical mixture drivers in the phase I mixtures. Water, food and human milk and blood extracts have been fractionated and each fraction tested in five selected bioassays. Currently, analytical determination of chemicals in the fractions is in progress.
In phase II of the project, we focus on children’s health by investigating associations between chemicals detected in human cord blood and reproductive and neuropsychological health in the children. We have selected 750 human cord blood samples from children in the Odense Child Cohort with relevant health information. The cord blood samples were selected based on available information on biomarkers for reproductive health and neuropsychological development (IQ, language development, ADHD) in the children. The chemical mixtures in these blood samples will be tested in seven bioassays, selected and based on phase I results.
On the theoretical side, we have started designing the user interface for calculation of mixture effects - Chemical Mixture Calculator 2.0. The intention is to develop a platform that allows for an initial, preliminary assessment of risk to a specific chemical mixture. We prioritize to have data on a large number of chemicals included in the system and to base the calculation of risk quotients on in vitro data linked to adverse outcomes and predicted human exposure data.
Moreover, we have started planning for four theoretical case studies (two on neurotoxicity and two on reproductive toxicity) on mixture risk assessment. The aim is to evaluate current safety margins and to provide input for the discussion of applying a mixture allocation factor in chemical risk assessment.
The project is progressing as expected and according to the deadlines with 19 submitted deliverables submitted and all milestones achieved.