Description du projet
Caractérisation de l’infection virale précoce
Les virus se propagent différemment dans les différents tissus et cellules après leur entrée initiale dans leurs hôtes. On ignore ce qui se passe aux premiers stades de l’infection cellulaire en raison des difficultés techniques que posent la détection et l’analyse d’une charge virale aussi faible. S’appuyant sur le développement récent d’une technologie d’imagerie pionnière pour les virus à ARN simple brin à polarité positive, le projet VirIm, financé par l’UE, élargira les capacités pour le groupe plus complexe des virus à ARN à polarité négative. En se concentrant sur le virus respiratoire syncytial, qui peut être mortel chez les nourrissons et les adultes vulnérables et pour lequel il n’existe aucun traitement efficace, l’équipe est parvenue à mieux comprendre la propagation virale précoce et son impact, ce qui ouvrira la voie à des traitements efficaces.
Objectif
Viruses are simple replicative units built from two components: a nucleic acid and a small number of associated proteins. Upon entering a cell, viruses co-opt the hosts machineries to copy their genetic material, while shutting down the hosts antiviral immune response. The success of viral infection differs dramatically from cell to cell through poorly understood mechanisms, resulting in heterogenous propagation through tissues, and ultimately, heterogeneous disease progression.
Although viruses have been studied for decades, the earliest steps of cellular infection have remained hidden, because only a few viral molecules are present at this stage, which presents formidable challenges for molecular analyses. Yet these initial events are critical for achieving a successful infection; transcription, translation and replication must be perfectly balanced to rapidly scale virus production before antiviral signalling pathways are activated. To overcome this barrier, we recently developed a first-in-kind imaging technology for simple positive-sense single stranded RNA (+ssRNA) viruses that transforms our ability to visualize early viral infection processes.
In this proposal, we will expand our single–molecule toolbox to gain molecular insights into early viral infection of the more complex group of negative-sense RNA (-ssRNA) viruses. We will focus on the respiratory syncytial virus (RSV), a -ssRNA virus which can be deadly in infants and vulnerable adults, and lacks effective treatments. Specifically, we will determine i) how viral transcription and replication are coordinated on single viral genomic RNA molecules to optimize early viral propagation, ii) what causes the early viral infection heterogeneity, and iii) how heterogeneity in early viral infection impacts infection outcome. Using our novel approaches, we will gain a deep understanding of viral biology, which will eventually inform therapeutic interventions.
Champ scientifique
Programme(s)
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Régime de financement
HORIZON-ERC - HORIZON ERC GrantsInstitution d’accueil
1011 JV AMSTERDAM
Pays-Bas