MainstreamBIO has successfully established and mobilized 7 regional MIPs in PL, DK, SE, BG, ES, IE, and NL, with the to-date participation of 93 actors in total (biomass producers, business, research, civil society and policy actors). To define how to best support the regional MIPs, a baseline analysis with over 300 respondents has been undertaken to spot and analyse the needs, socio-economic context and framework conditions in target rural areas. To enhance understanding of the possibilities for bio-based and nutrient recycling solutions adoption, an analysis was undertaken of value chains in the 7 regions, which has mapped 27 value chains in terms of available biomass, waste streams and flows and actors involved. A catalogue of 16 technologies, 34 business models and 19 social innovations related to small-scale bio-based solutions has been developed, along with an inventory of 31 nutrient recycling practices, collecting info that can facilitate practical use while providing inspiration and guidance for rural actors. A multi-criteria decision support system has been developed for matching available biomass with waste streams and market and technology to guide rural actors to identify solutions that best fit their territorial dynamics, resource availability and socio-economic context. The multi-criteria decision support system has been integrated in the MainstreamBIO Digital toolkit, a user-friendly, online platform offering guidance, knowledge and networking for small-scale bio-based solutions, comprising of the following main components: catalogue of small-scale bio-based solutions, collection of nutrient recycling best practices, Decision Support System, Bioeconomy Repository with over than 500 resources, Tool Library gathering 7 tools from relevant past and present initiatives, and BioForum. A portfolio of 10 innovation technical and business support services has been developed, through a co-creation, participatory approach. In total 28 initiatives have been identified through open calls in our 7 MIPs to receive tailored innovation support services of their preference, involving 36 bio-based products/ services and 32 innovators in total . The provision of services is ongoing and results and conclusions will be analysed through the developed monitoring and evaluation framework and tools to fuel iterative improvement in the 2nd innovation round.