The EU-funded Engage4BIO project was strengthening circular, sustainable bioeconomy and sustainable regional development through engaging quadruple helix actors (taking into account their diversity of societal, economic and cultural perspectives) in five regional bio-based systems (hubs) in processes of design thinking, co-creation, (re)training and skills development. This is crucial for the updated European Bioeconomy strategy (2018) and its outlined need to launch actions for the deployment of local bioeconomies and new ways to govern the required societal transformation and at the same time engage citizens through awareness raising and education on sustainable production, consumption and lifestyles. The hubs focused on five major value chains, namely circular and bio-based textiles (NL), agriculture and agro-food industries (HU), wood and interior (AT), bio-based and sustainable packaging (FI) and blue bioeconomy (IT). Based on regional specificities, activities have been co-created, and have been performed and (re-) shaped ensuring inclusiveness of all actors. The inclusion of art and design creatively enhanced the ability to connect, integrate and bridge quadruple helix actors. The project outputs resulted in guidelines for regional analysis, trainings, governance, outreach and co-creation transferrable to European regions.The results are fully open to the public. Thus, exploitability reaches beyond the targeted audience and allows access beyond borders.