Descrizione del progetto
Uno studio sulla cinetica della crescita delle colture microbiche miste
La teoria dello stato di transizione microbica offre un modello matematico per descrivere la crescita microbica, collegando equilibri termodinamici e tassi di crescita. Tuttavia, questa nuova teoria non è in grado di descrivere sufficientemente le colture microbiche miste trovate nei sistemi di trattamento delle acque reflue. Il progetto MTSonNAS, finanziato dall’UE, ricorrerà alla teoria dello stato di transizione microbica per studiare la cinetica della crescita di due diversi batteri nitrificanti. Verrà inoltre esaminata l’inibizione della crescita batterica a opera di diversi tipi di inibitori, con l’obiettivo di testare questa nuova teoria per diverse associazioni all’interno di colture microbiche miste in diverse condizioni ambientali. I risultati del progetto rafforzeranno la comprensione dei processi di crescita microbica nei sistemi di trattamento ingegnerizzati e naturali.
Obiettivo
Traditional engineering models known as Activated Sludge Model (ASM) are commonly used for operation, design, and optimization of wastewater treatment plants. ASM predict microbial growth with simulation and calibration of model parameters which are bound to a range of experimental conditions. Recently, Microbial Transition State (MTS) approach was proposed which is a statistical-physics based theory of microbial growth linking thermodynamic balances and growth rates. A growth equation was derived from these first principles, which links a flux (the growth of microbes) to a force, i.e. the Gibbs free potential energy density in medium contained in randomly distributed substrate molecules. MTS has not been fully verified with experimental data on mixed microbial cultures such as sustained in wastewater treatment systems. As the theory is developed for describing standard microbial growth, conditions such as inhibition of growth by any pollutant compound has not been considered/applied in real systems. The aim of the research is to investigate growth kinetics of two different nitrifying bacteria individually (Ammonia and Nitrite oxidizers) with MTS modeling approach and identify nitirification growth kinetics. Inhibition of bacterial growth with different types of inhibitors will also be investigated in order to evaluate and verify application of MTS model for different guilds in mixed microbial cultures under different environmental conditions. The outcomes of the study will contribute to understanding and control of microbial growth processes in engineered and natural treatment systems. Identification of inhibition kinetics will be used for mitigation of pollution generated from industrial processes as well as theoretical development of MTS theory to be applied in more complex biological process applications. The project is an intersection of environmental engineering, molecular biology, and modeling disciplines which will contribute Green Deal Strategy of EU.
Campo scientifico
- engineering and technologyenvironmental engineeringwater treatment processeswastewater treatment processes
- natural sciencesbiological sciencesmicrobiologybacteriology
- natural sciencesearth and related environmental sciencesenvironmental sciencespollution
- natural sciencesbiological sciencesmolecular biology
Programma(i)
Argomento(i)
Meccanismo di finanziamento
HORIZON-AG-UN - HORIZON Unit GrantCoordinatore
34469 Maslak, Istanbul
Turchia