Cellevat3d® nanofiber microcarriers provide a sustainable, scalable solution for upstream bioprocessing. They enable homogeneous 3D dense cultures with high yields while reducing shear forces in bioreactors. Benchmarking data revealed high transfection efficiency and volumetric productivity, achieving a 3.1-fold increase in AAV9 vector yield compared to current microcarriers. These advancements enhance gene therapy production strategies by reducing costs and improving accessibility to biologics.
Stem cell applications were also explored, with Cellevat3d® macrocarriers achieving a 19-fold increase in hMSC density within 8 days while maintaining >90% cell viability. Retrieved cells retained morphology, proliferation rate, and key stemness markers, confirming high-quality production suitable for cell therapy applications.
Two IP filings were submitted:
• EP24180000.2: Process for culturing stem cells
• EP24155092.0: Production of AAVs.
These patents facilitated customer validations, with 15 out of 31 partners engaged, 11 receiving materials, and 6 generating critical launch data. Early data enabled Cellevat3d®’s nanofiber microcarriers’ global launch a year ahead of schedule in November 2024. The product is now available for order at cellevate.com.
The project’s success accelerated Cellevate’s commercialization phase, supported by a €3.3M rights issue with participation from Industrifonden, EIC Fund, and Onsight Ventures. Preparations for a Series A funding round (€15M) in mid-2025 are underway to scale production, support commercialization, and meet increasing demand in the EU and US.
Cellevate is now focusing on:
1. Scaling up production to meet customer requirements.
2. Expanding the Cellevat3d® product portfolio for large-scale viral vector and stem cell applications.
3. Strengthening market access, stakeholder engagement, and scientific leadership.
The Cellevat3d® platform delivers innovative, high-performance solutions for next-generation therapies. With demonstrated increases in productivity, cost-effectiveness, and scalability, the platform addresses critical biomanufacturing challenges. By accelerating market readiness and achieving milestones ahead of schedule, Cellevate is well-positioned to drive the biomanufacturing revolution, enhancing access to life-changing biologics worldwide.
