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Structural Basis of Ubiquitin Protein Ligation

Obiettivo

The attachment of Ubiquitin (Ub) to proteins (ubiquitination) is one of the most abundant post‐translational modifications in eukaryotes. Deregulation of the ubiquitination reaction is the cause of various diseases, including cancer. Ubiquitination is catalyzed by the sequential action of an activating (E1), a conjugating (E2), and a ligating (E3) enzyme. Ubiquitination enzymes contain catalytic cysteine residues that transfer Ub via thioester intermediates to a substrate lysine residue. The functional consequences of ubiquitination depend on the length and linkage of the attached Ub chain. While poly-ubiquitination commonly targets proteins for proteasomal degradation, mono- or multi-ubiquitination of receptor proteins serve as internalization or sorting signals. Interestingly, Ub ligases mediate both mono- and poly-ubiquitination depending on their substrates or other modulators. Although the basic principles of ubiquitination have been identified, the exact reaction mechanism underlying Ub protein ligation is still unknown. Furthermore, it is still unclear what determines whether a substrate is mono-, multi- or poly-ubiquitinated. To provide a structural basis of the ubiquitin protein ligation, we propose to use NMR spectroscopy in combination with biochemical tools to identify and characterize conformational changes and binding surfaces in HECT‐type Ub ligases at different steps of the ubiquitination reaction.

Invito a presentare proposte

FP7-PEOPLE-IRG-2008
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Coordinatore

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
Contributo UE
€ 100 000,00
Indirizzo
HOFGARTENSTRASSE 8
80539 Munchen
Germania

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Regione
Bayern Oberbayern München, Kreisfreie Stadt
Tipo di attività
Research Organisations
Contatto amministrativo
Vlasta Wirth (Ms.)
Collegamenti
Costo totale
Nessun dato