Cel
The Affinomics programme aims to leverage existing efforts in Europe to generate large-scale resources of validated protein-binding molecules (‘binders’) as affinity reagents for characterisation of the human proteome and to apply them in comprehensive structural and functional analyses of protein expression, interactions and complexes. Proteome targets will be focused on five categories of inter-related human proteins involved in signal transduction, cell regulation and cancer, namely protein kinases, SH2 domain-containing proteins, protein tyrosine phosphatases, proteins somatically mutated in cancers and candidate cancer biomarkers. Binders to about 1000 protein targets will be made over the course of the programme. A high throughput, coordinated production pipeline for antigens and binders will be established. Target antigens will be expressed in three forms, as folded full-length proteins or domains, as large peptide fragments (PrESTs) based on low homology to other human proteins and as small peptides, in some cases phosphorylated. Binder types to be generated include affinity-purified polyclonal antibodies, monoclonal antibodies, recombinant antibody fragments and non-immunoglobulin scaffolds. An important aspect will be the development of highly efficient ‘next generation’ recombinant selection methods, based on phage, cell and ribosome display, capable of producing high quality binders at greater throughput and lower cost than hitherto. Systems and procedures for thorough binder validation and quality control will be established. The affinity reagents will be applied in advanced innovative and sensitive technologies for specific detection of target proteins and interacting protein complexes in cells, tissues and fluids, for improved understanding of protein function and new classes of diagnostic assays.
Dziedzina nauki
Temat(-y)
Zaproszenie do składania wniosków
FP7-HEALTH-2009-two-stage
Zobacz inne projekty w ramach tego zaproszenia
System finansowania
CP-IP - Large-scale integrating projectKoordynator
CB22 3AT Cambridge
Zjednoczone Królestwo
Zobacz na mapie
Uczestnicy (19)
100 44 Stockholm
Zobacz na mapie
8006 ZURICH
Zobacz na mapie
22100 LUND
Zobacz na mapie
1165 Kobenhavn
Zobacz na mapie
02044 VTT ESPOO
Zobacz na mapie
69117 Heidelberg
Zobacz na mapie
00133 Roma
Zobacz na mapie
CB22 3AT CAMBRIDGE
Zobacz na mapie
38106 Braunschweig
Zobacz na mapie
34125 Kassel
Zobacz na mapie
85764 Neuherberg
Zobacz na mapie
NW1 2BE London
Zobacz na mapie
69120 Heidelberg
Zobacz na mapie
9052 ZWIJNAARDE - GENT
Zobacz na mapie
17177 Stockholm
Zobacz na mapie
OX1 2JD Oxford
Zobacz na mapie
72074 Tuebingen
Zobacz na mapie
751 05 Uppsala
Zobacz na mapie
EN5 2HA LONDON
Zobacz na mapie