Final Report Summary - CSRR (Correlative Super Resolution and Real-Time Imaging of Herpes Virus Infection)
In the course of the project we have successfully:
1. Implemented the correlative live-cell and super-resolution imaging method by developing protocols for on-stage in situ sample fixation, labeling and algorithms for precise image registration.
2. Automated the correlative live-cell and super-resolution imaging method through the use of microfluidic devices, which has allowed us to streamline the sample preparation and improve throughput.
3. Applied the correlative live-cell and super-resolution imaging method to important biological problems, such as studying the impact of cellular roadblocks on cargo transport and studying correlations between mitochondria dynamics and morphology.
4. Increased the information content of the correlative live-cell and super-resolution imaging method by developing ways to extract precise quantitative information from super-resolution images.
Overall, the project has allowed the development of novel imaging technologies expanding the repertoire of available single molecule techniques and opening the doors for applying these tools to several other biological questions. Therefore, it is expected to make a big impact in microscopy, biophysics and biology communities.