Cel
Embryonic stem (ES) cells have the potential to differentiate into any type of cell as well as to renew indefinitely in culture. They hold great potential for the development of personalized medicines. However, the molecular mechanisms underpinning cell fate decisions by individual cells are poorly understood. There is compelling evidence that two large multi-protein machines, the Nucleosome Remodelling and Deacetylation (NuRD) complex and the Polycomb Repressive Complexes (PRCs), modulate chromatin structure to control stem cell renewal, lineage commitment and differentiation. Moreover, they are clearly implicated in cancer.
The goal of 4DCellFate is to understand how the PRC/NuRD complexes and their plethora of interactions (protein/protein, protein/nucleosome, protein/nucleic acids) regulate cell fate. To obtain this global, quantitative and dynamic – 4D – understanding of the structure/functions of these two multi-protein machines during ES cell differentiation and in different disease states, we propose a large scale multi-disciplinary “data-gathering” approach combining European excellence in interactomics (affinity purification, quantitative mass spectrometry-based proteomics, ChIP-seq analysis, light microscopy), structural biology (X-ray crystallography, NMR, native-state mass spectrometry, Electron Microscopy, biochemistry/biophysics), cellular, tumour, and computational biology. We expect to significantly advance the technology, mainly in the fields of proteomics, structural biology, and small molecule screening, and to make our knowledge, reagents and data publicly available to the scientific community.
The ultimate outcome of 4DCellFate will be to lay the foundations for understanding the role of the PRC/NuRD complexes in ES cell differentiation and cancer, specifically in leukaemogenesis. Three SMEs and one large multi-national pharma will be actively involved ensuring that our findings can be translated into new ways to control complex diseases.
Dziedzina nauki
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomics
- natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acids
- natural sciencesphysical sciencesopticsmicroscopyelectron microscopy
- medical and health sciencesmedical biotechnologycells technologiesstem cells
- natural sciencesbiological sciencesmolecular biologystructural biology
Zaproszenie do składania wniosków
FP7-HEALTH-2011-two-stage
Zobacz inne projekty w ramach tego zaproszenia
System finansowania
CP-IP - Large-scale integrating projectKoordynator
08003 Barcelona
Hiszpania
Zobacz na mapie
Uczestnicy (12)
CB2 1TN Cambridge
Zobacz na mapie
1165 Kobenhavn
Zobacz na mapie
Zakończenie uczestnictwa
3584 CX Utrecht
Zobacz na mapie
2000 Antwerpen
Zobacz na mapie
69117 Heidelberg
Zobacz na mapie
80539 Munchen
Zobacz na mapie
20121 Milano
Zobacz na mapie
CB25 9TL Cambridge
Zobacz na mapie
8000 Aarhus
Zobacz na mapie
Zakończenie uczestnictwa
413 46 Göteborg
Zobacz na mapie
TW89GS Brentford
Zobacz na mapie
6525 XZ Nijmegen
Zobacz na mapie