Obiettivo Laboratory-on-a-Chip technology was introduced in this field. To avoid the complexity of an animal model and to reduce the number of animals for pre-clinical research cell culture models are important. Here, the combination of microfluidics, tissue engineering and neuroelectrophysiology on MEA-chips is suggested. Because neuronal tissue on chip may act differently from the neurons in their natural environment, the first objective is to follow a systems engineering approach to realize a platform technology, which allows us to reliably co-culture cells in a 3D interconnected configuration, providing an artificially vascularized system on a MEA. For on-line monitoring of the culturing conditions, we will implement micro-total analysis systems (TAS) technology proposing microchip capillary electrophoresis, potentially coupled to mass spectrometry, to correlate electrophysiology with neurochemistry. Previously, it has been demonstrated that physical and chemical micro- and nanostructures influence cell guidance, viability and cell differentiation, so far, unfortunately without a unifying theory to explain the involved mechanisms. Therefore, our second objective is to further our understanding with respect to the influence of nanocues, implementing microfluidic programming to activate porous nanostructures on MEA and investigate cellular signaling and pathway reactions related to the cell’s adhesion mechanism. Combining the first and the second objective will allow us to work towards clinical questions of neurodynamic diseases as epilepsy, characterized by intermittent abnormal synchronization of different neuronal populations. We hypothesize that for these disorders, 3D cell co-culture models will resemble the natural neural networks more closely than 2D, which may subsequently serve as a model to study novel therapeutic procedures, for instance selective neurostimulation. Thus, we propose, as our third objective, nanostimulation of neuronal subsystem. Campo scientifico natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidicsmedical and health sciencesmedical biotechnologytissue engineeringnatural scienceschemical scienceselectrochemistryelectrophoresisnatural scienceschemical sciencesanalytical chemistrymass spectrometrynatural sciencescomputer and information sciencesartificial intelligencecomputational intelligence Programma(i) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) ERC-SG-PE8 - ERC Starting Grant - Products and process engineering Invito a presentare proposte ERC-2011-StG_20101014 Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-SG - ERC Starting Grant Istituzione ospitante TECHNISCHE UNIVERSITEIT EINDHOVEN Contributo UE € 517 800,00 Indirizzo GROENE LOPER 3 5612 AE Eindhoven Paesi Bassi Mostra sulla mappa Regione Zuid-Nederland Noord-Brabant Zuidoost-Noord-Brabant Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo J.L.F.C.M. Snelders (Mr.) Ricercatore principale Regina Luttge (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato Beneficiari (2) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto TECHNISCHE UNIVERSITEIT EINDHOVEN Paesi Bassi Contributo UE € 517 800,00 Indirizzo GROENE LOPER 3 5612 AE Eindhoven Mostra sulla mappa Regione Zuid-Nederland Noord-Brabant Zuidoost-Noord-Brabant Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo J.L.F.C.M. Snelders (Mr.) Ricercatore principale Regina Luttge (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato UNIVERSITEIT TWENTE Paesi Bassi Contributo UE € 742 200,00 Indirizzo DRIENERLOLAAN 5 7522 NB Enschede Mostra sulla mappa Regione Oost-Nederland Overijssel Twente Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Ferdinand Damhuis (Mr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato