Obiettivo The skeleton and the sinusoidal vasculature form a functional unit with great relevance in health, regeneration, and disease. Currently, fundamental aspects of sinusoidal vessel growth, specialization, arteriovenous organization and the consequences for tissue perfusion, or the changes occurring during ageing remain unknown. Our preliminary data indicate that key principles of bone vascularization and the role of molecular regulators are highly distinct from other organs. I therefore propose to use powerful combination of mouse genetics, fate mapping, transcriptional profiling, computational biology, confocal and two-photon microscopy, micro-CT and PET imaging, biochemistry and cell biology to characterize the growth, differentiation, dynamics, and ageing of the bone vasculature. In addition to established angiogenic pathways, the role of highly promising novel candidate regulators will be investigated in endothelial cells and perivascular osteoprogenitors with sophisticated inducible and cell type-specific genetic methods in the mouse. Complementing these powerful in vivo approaches, 3D co-cultures generated by cell printing technologies will provide insight into the communication between different cell types. The dynamics of sinusoidal vessel growth and regeneration will be monitored by two-photon imaging in the skull. Finally, I will explore the architectural, cellular and molecular changes and the role of capillary endothelial subpopulations in the sinusoidal vasculature of ageing and osteoporotic mice.Technological advancements, such as new transgenic strains, mutant models or cell printing approaches, are important aspects of this proposal. AngioBone will provide a first conceptual framework for normal and deregulated function of the bone sinusoidal vasculature. It will also break new ground by analyzing the role of blood vessels in ageing and identifying novel strategies for tissue engineering and, potentially, the prevention/treatment of osteoporosis. Campo scientifico natural sciencesbiological sciencesbiochemistrynatural sciencesbiological sciencesgeneticsmedical and health sciencesclinical medicineradiologynuclear medicinenatural sciencesbiological sciencescell biologymedical and health sciencesmedical biotechnologytissue engineering Programma(i) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) ERC-AG-LS3 - ERC Advanced Grant - Cellular and Developmental Biology Invito a presentare proposte ERC-2013-ADG Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-AG - ERC Advanced Grant Istituzione ospitante UNIVERSITAET MUENSTER Contributo UE € 2 478 750,40 Indirizzo SCHLOSSPLATZ 2 48149 MUENSTER Germania Mostra sulla mappa Regione Nordrhein-Westfalen Münster Münster, Kreisfreie Stadt Tipo di attività Higher or Secondary Education Establishments Ricercatore principale Ralf Heinrich Adams (Prof.) Contatto amministrativo Katharina Steinberg (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato Beneficiari (1) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto UNIVERSITAET MUENSTER Germania Contributo UE € 2 478 750,40 Indirizzo SCHLOSSPLATZ 2 48149 MUENSTER Mostra sulla mappa Regione Nordrhein-Westfalen Münster Münster, Kreisfreie Stadt Tipo di attività Higher or Secondary Education Establishments Ricercatore principale Ralf Heinrich Adams (Prof.) Contatto amministrativo Katharina Steinberg (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato