Final Activity Report Summary - TELOMERASE (Identification and functional assessment of genes specifically involved in hTERT regulation)
This project, funded by a Marie Curie EIF during two years (University of Bergen, Norway), has allowed the identification of eight transcription factors rapidly induced by retinoids (vitamin A derivatives) and potentially important in telomerase regulation. These eight transcription factors, early-induced by retinoic acid, have been identified by a microarray approach (DNA biochips) and their expression confirmed with different techniques of biochemistry and molecular biology. Our goal is now to assess the contribution of these eight candidate genes in telomerase regulation leukemic cells, by studying the consequence of their over-expression and/or specific invalidation (by RNA interference). All the molecular and cellular tools required for the functional study of these candidate genes have been developed in the perspective of my reintegration in France (also granted by a European contract) were the project will be continued. These genes could act as tumour suppressors and could have potential applications in clinical oncology (diagnostics, prognostic and therapeutics).