Substrate import at work: single-molecule studies of ABC transporters

Informazioni relative al progetto

SM-IMPORT

ID dell’accordo di sovvenzione: 638536

DOI 10.3030/638536

Progetto chiuso

Data della firma CE 6 Marzo 2015

Data di avvio 1 Settembre 2015

Data di completamento 30 Giugno 2021

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale € 1 500 000,00

Contributo UE € 1 500 000,00

Coordinato da

LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN
Germany

Pubblicazioni

Peer reviewed articles (21)

Single-Molecule Observation of Ligand Binding and Conformational Changes in FeuA

Autori: Marijn de Boer, Giorgos Gouridis, Yusran Abdillah Muthahari, Thorben Cordes
Pubblicato in: Biophysical Journal, Numero 117/9, 2019, Pagina/e 1642-1654, ISSN 0006-3495
Editore: Biophysical Society
DOI: 10.1016/j.bpj.2019.08.005

Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers

Autori: Marijn de Boer, Giorgos Gouridis, Ruslan Vietrov, Stephanie L Begg, Gea K Schuurman-Wolters, Florence Husada, Nikolaos Eleftheriadis, Bert Poolman, Christopher A McDevitt, Thorben Cordes
Pubblicato in: eLife, Numero 8, 2019, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.44652

FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

Autori: Eitan Lerner, Anders Barth, Jelle Hendrix, Benjamin Ambrose, Victoria Birkedal, Scott C Blanchard, Richard Börner, Hoi Sung Chung, Thorben Cordes, Timothy D Craggs, Ashok A Deniz, Jiajie Diao, Jingyi Fei, Ruben L Gonzalez, Irina V Gopich, Taekjip Ha, Christian A Hanke, Gilad Haran, Nikos S Hatzakis, Sungchul Hohng, Seok-Cheol Hong, Thorsten Hugel, Antonino Ingargiola, Chirlmin Joo, Achillefs N Ka
Pubblicato in: eLife, Numero 10, 2021, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.60416

ColiCoords: A Python package for the analysis of bacterial fluorescence microscopy data

Autori: Jochem H. Smit, Yichen Li, Eliza M. Warszawik, Andreas Herrmann, Thorben Cordes
Pubblicato in: PLOS ONE, Numero 14/6, 2019, Pagina/e e0217524, ISSN 1932-6203
Editore: Public Library of Science
DOI: 10.1371/journal.pone.0217524

Characterization of fluorescent proteins with intramolecular photostabilization

Autori: Sarah S. Henrikus; Konstantinos Tassis; Lei Zhang; Jasper H. M. van der Velde; Christian Gebhardt; Andreas Herrmann; Gregor Jung; Thorben Cordes
Pubblicato in: ChemBioChem, Numero 1, 2021, Pagina/e 1-2, ISSN 1439-7633
Editore: Wiley-VCH
DOI: 10.1101/2020.03.07.980722

Self-Healing Dyes—Keeping the Promise?

Autori: Michael Isselstein, Lei Zhang, Viktorija Glembockyte, Oliver Brix, Gonzalo Cosa, Philip Tinnefeld, Thorben Cordes
Pubblicato in: The Journal of Physical Chemistry Letters, Numero 11/11, 2020, Pagina/e 4462-4480, ISSN 1948-7185
Editore: American Chemical Society
DOI: 10.1021/acs.jpclett.9b03833

ABCE1 Controls Ribosome Recycling by an Asymmetric Dynamic Conformational Equilibrium

Autori: Giorgos Gouridis, Bianca Hetzert, Kristin Kiosze-Becker, Marijn de Boer, Holger Heinemann, Elina Nürenberg-Goloub, Thorben Cordes, Robert Tampé
Pubblicato in: Cell Reports, Numero 28/3, 2019, Pagina/e 723-734.e6, ISSN 2211-1247
Editore: Cell Press
DOI: 10.1016/j.celrep.2019.06.052

Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer

Autori: Eitan Lerner, Thorben Cordes, Antonino Ingargiola, Yazan Alhadid, SangYoon Chung, Xavier Michalet, Shimon Weiss
Pubblicato in: Science, Numero 359/6373, 2018, Pagina/e eaan1133, ISSN 0036-8075
Editore: American Association for the Advancement of Science
DOI: 10.1126/science.aan1133

Konformationsbewegungen von aktiven Membrantransportern

Autori: Rebecca Mächtel, Christian Gebhardt, Thorben Cordes
Pubblicato in: BIOspektrum, Numero 24/5, 2018, Pagina/e 495-497, ISSN 0947-0867
Editore: Spektrum Akademischer Verlag GmbH
DOI: 10.1007/s12268-018-0945-2

Self-healing dyes for super-resolution fluorescence microscopy

Autori: Jasper H M van der Velde, Jochem H Smit, Elke Hebisch, Michiel Punter, Thorben Cordes
Pubblicato in: Journal of Physics D: Applied Physics, Numero 52/3, 2019, Pagina/e 034001, ISSN 0022-3727
Editore: Institute of Physics Publishing
DOI: 10.1088/1361-6463/aae752

On the impact of competing intra- and intermolecular triplet-state quenching on photobleaching and photoswitching kinetics of organic fluorophores

Autori: Jochem H. Smit, Jasper H. M. van der Velde, Jingyi Huang, Vanessa Trauschke, Sarah S. Henrikus, Si Chen, Nikolaos Eleftheriadis, Eliza M. Warszawik, Andreas Herrmann, Thorben Cordes
Pubblicato in: Physical Chemistry Chemical Physics, Numero 21/7, 2019, Pagina/e 3721-3733, ISSN 1463-9076
Editore: Royal Society of Chemistry
DOI: 10.1039/c8cp05063e

Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study

Autori: Björn Hellenkamp, Sonja Schmid, Olga Doroshenko, Oleg Opanasyuk, Ralf Kühnemuth, Soheila Rezaei Adariani, Benjamin Ambrose, Mikayel Aznauryan, Anders Barth, Victoria Birkedal, Mark E. Bowen, Hongtao Chen, Thorben Cordes, Tobias Eilert, Carel Fijen, Christian Gebhardt, Markus Götz, Giorgos Gouridis, Enrico Gratton, Taekjip Ha, Pengyu Hao, Christian A. Hanke, Andreas Hartmann, Jelle Hendrix, Lass
Pubblicato in: Nature Methods, Numero 15/9, 2018, Pagina/e 669-676, ISSN 1548-7091
Editore: Nature Publishing Group
DOI: 10.1038/s41592-018-0085-0

Conformational dynamics of the ABC transporter McjD seen by single‐molecule FRET

Autori: Florence Husada, Kiran Bountra, Konstantinos Tassis, Marijn de Boer, Maria Romano, Sylvie Rebuffat, Konstantinos Beis, Thorben Cordes
Pubblicato in: The EMBO Journal, Numero 37/21, 2018, Pagina/e e100056, ISSN 0261-4189
Editore: Nature Publishing Group
DOI: 10.15252/embj.2018100056

Single-molecule FRET reveals the pre-initiation and initiation conformations of influenza virus promoter RNA

Autori: Nicole C. Robb, Aartjan J. W. te Velthuis, Ralph Wieneke, Robert Tampé, Thorben Cordes, Ervin Fodor, Achillefs N. Kapanidis
Pubblicato in: Nucleic Acids Research, Numero (2016) 44 (21): 10304-10315, 2016, Pagina/e gkw884, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkw884

A Quantitative Theoretical Framework For Protein-Induced Fluorescence Enhancement–Förster-Type Resonance Energy Transfer (PIFE-FRET)

Autori: Eitan Lerner, Evelyn Ploetz, Johannes Hohlbein, Thorben Cordes, Shimon Weiss
Pubblicato in: The Journal of Physical Chemistry B, Numero 120/26, 2016, Pagina/e 6401-6410, ISSN 1520-6106
Editore: American Chemical Society
DOI: 10.1021/acs.jpcb.6b03692

Förster resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers

Autori: Evelyn Ploetz, Eitan Lerner, Florence Husada, Martin Roelfs, SangYoon Chung, Johannes Hohlbein, Shimon Weiss, Thorben Cordes
Pubblicato in: Scientific Reports, Numero 6/1, 2016, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/srep33257

A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization

Autori: Jasper H. M. van der Velde, Jens Oelerich, Jingyi Huang, Jochem H. Smit, Atieh Aminian Jazi, Silvia Galiani, Kirill Kolmakov, Giorgos Guoridis, Christian Eggeling, Andreas Herrmann, Gerard Roelfes, Thorben Cordes
Pubblicato in: Nature Communications, Numero 7, 2016, Pagina/e 10144, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/ncomms10144

Caging and Photoactivation in Single-Molecule Förster Resonance Energy Transfer Experiments

Autori: Atieh Aminian Jazi, Evelyn Ploetz, Muhamad Arizki, Balasubramaniam Dhandayuthapani, Izabela Waclawska, Reinhard Krämer, Christine Ziegler, Thorben Cordes
Pubblicato in: Biochemistry, Numero 56/14, 2017, Pagina/e 2031-2041, ISSN 0006-2960
Editore: American Chemical Society
DOI: 10.1021/acs.biochem.6b00916

Molecular structure, DNA binding mode, photophysical properties and recommendations for use of SYBR Gold

Autori: Pauline J Kolbeck, Willem Vanderlinden, Gerd Gemmecker, Christian Gebhardt, Martin Lehmann, Aidin Lak, Thomas Nicolaus, Thorben Cordes, Jan Lipfert
Pubblicato in: Nucleic Acids Research, Numero 49/9, 2021, Pagina/e 5143-5158, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkab265

Single‐molecule studies of conformational states and dynamics in the ABC importer OpuA

Autori: Konstantinos Tassis, Ruslan Vietrov, Matthijs Koning, Marijn Boer, Giorgos Gouridis, Thorben Cordes
Pubblicato in: FEBS Letters, Numero 595/6, 2021, Pagina/e 717-734, ISSN 0014-5793
Editore: Elsevier BV
DOI: 10.1002/1873-3468.14026

An integrated transport mechanism of the maltose ABC importer

Autori: Rebecca Mächtel, Alessandra Narducci, Douglas A. Griffith, Thorben Cordes, Cédric Orelle
Pubblicato in: Research in Microbiology, Numero 170/8, 2019, Pagina/e 321-337, ISSN 0923-2508
Editore: Elsevier BV
DOI: 10.1016/j.resmic.2019.09.004