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Mixed brushes of stimuli-responsive polymers for the selective adsorption of proteins

Cel

Advanced materials are at the heart of many scientific fields, particularly in the biomedical and biotechnological areas. Many devices (e.g. biosensors, implants, catheters, and systems for drug delivery) would benefit from an effective control over molecular and cellular interactions at material surfaces. Main challenges in this field include the control of biomolecules (e.g. proteins, DNA) adsorption on different surfaces. Designing surfaces that allow proteins to be adsorbed in a controlled manner is particularly important and challenging. Many strategies were recently developed to spatially or temporally control protein adsorption.
In this project, a novel strategy for selective protein adsorption from a mixture of proteins will be developed. This strategy will be based on the use of mixed polymer brushes sensitive to ionic strength and pH of the surrounding medium. The project rests on the following steps:
i. The design of mixed polymer brushes whose properties will be tuned by adjusting pH and ionic strength of the medium. The influence of polymer properties (molecular weight, and degree of dissociation and conformation through pH and ionic strength variations) on protein adsorption will be studied.
ii. The development of a procedure, based on time-of-flight secondary ion mass spectrometry (ToF-SIMS), to distinguish different proteins adsorbed together on model interfaces.
iii. The use of this ToF-SIMS-based procedure to investigate the potential of the created mixed brushes to selectively adsorb one protein, at first from a mixture of two or three proteins, and then from a more complex medium.
The results obtained in this work will be significant for material science and biomedical applications through the development of a robust procedure to prepare “smart” surfaces with novel properties to control protein adsorption.

Dziedzina nauki (EuroSciVoc)

Program(-y)

Temat(-y)

System finansowania

MSCA-IF-EF-ST - Standard EF

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Zaproszenie do składania wniosków

(odnośnik otworzy się w nowym oknie) H2020-MSCA-IF-2014

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Koordynator

UNIVERSITE CATHOLIQUE DE LOUVAIN
Wkład UE netto
€ 172 800,00
Adres
PLACE DE L UNIVERSITE 1
1348 LOUVAIN LA NEUVE
Belgia
Region
Région de Bruxelles-Capitale/Brussels Hoofdstedelijk Gewest Région de Bruxelles-Capitale/ Brussels Hoofdstedelijk Gewest Arr. de Bruxelles-Capitale/Arr. Brussel-Hoofdstad
Rodzaj działalności
Higher or Secondary Education Establishments
Linki
Koszt całkowity
€ 172 800,00