Projektbeschreibung
Revolutionierung diagnostischer Tests
Bei der Analyse und Entdeckung neuer resistenter Erreger und Infektionskrankheiten sind diagnostische Tests von entscheidender Bedeutung, um effiziente Schlussfolgerungen zu ziehen. Leider verwenden die derzeitigen Diagnosetechniken entweder Kultivierung oder die Polymerase-Kettenreaktion, was zu schwerwiegenden Nachteilen führt und oft Probleme bei der Charakterisierung neuer Krankheitserreger innerhalb der 3-Stunden-Grenze mit sich bringt. Das EU-finanzierte Projekt MARA zielt darauf ab, dieses Problem durch die Entwicklung und Einführung eines revolutionären Durchbruchs auf der Grundlage dreier neuartiger Technologien zu lösen. Diese werden auch die Herstellung eines DNA-basierten molekularen Instrumentariums zur Charakterisierung von Krankheitserregern demonstrieren. Dazu werden sie nicht nur die Technologie der autonomen Detektion von Nukleinsäuren nutzen, die keine komplizierte Ausrüstung erfordert, sondern auch einen neuen Durchbruch in der Proteinnachahmung und Enzymerzeugung sowie einen molekularen Roboter, der Zellen identifizieren und zerstören kann.
Ziel
Diagnostic tests are essential to provide a targeted treatment of infectious diseases and to contain the further spread of multidrug resistant pathogens. Current methods are based either on cultivation or on PCR and have significant limitations concerning the clinical requirements to characterise pathogens including their resistance mechanisms within 3 hours. In MARA, we will develop and combine three radically novel technologies that will lead to substantial breakthroughs in science, medicine and industry and, as proof-of principle, use them to create a DNA-based molecular toolkit characterising pathogens.
First, the detection of pathogen-associated antigens will be performed by Autonomous Detection Nucleic Acids (AUDENA) that are independent of any laboratory instruments and sophisticated processing. The realisation of the AUDENA concept will lead to an autonomous, stable, simple and very economic novel sensor class applicable for any water-soluble substances. The second revolutionary technology in MARA employs a novel approach in protein mimicry and creation of artificial enzymes, which represents a breakthrough in several disciplines, such as biotechnology, biomedical manufacturing and the energy sector. The third breakthrough in this project represents the development of a Molecular Robot (MORO) that can specifically identify target cells and destroy them. In MARA, the MORO will be used for the lysis of bacterial cells to release intracellular antibiotic resistance associated antigens, but the long-term vision anticipates an application as antibiotic replacement for infectious diseases and a therapeutic agent for cancer treatment, which would represent one of the most important breakthroughs in medicine in the recent years.
To meet the highly ambitious objectives pointed out in this proposal, MARA is driven by a complementary, multidisciplinary team of leading experts, with a young, high-profile scientist in the lead.
Wissenschaftliches Gebiet
- natural sciencesbiological sciencesgeneticsDNA
- medical and health scienceshealth sciencesinfectious diseases
- medical and health sciencesbasic medicinepharmacology and pharmacydrug resistancemultidrug resistance
- medical and health sciencesbasic medicinepharmacology and pharmacydrug resistanceantibiotic resistance
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
Programm/Programme
Thema/Themen
Aufforderung zur Vorschlagseinreichung
Andere Projekte für diesen Aufruf anzeigenUnterauftrag
H2020-FETOPEN-2014-2015-RIA
Finanzierungsplan
RIA - Research and Innovation actionKoordinator
1210 Wien
Österreich