Periodic Reporting for period 2 - PROSEQO (PROtein SEQuencing using Optical single molecule real-time detection)
Okres sprawozdawczy: 2017-03-01 do 2019-02-28
The highly interdisciplinary effort associated with PROSEQO will lead to:
· development of advanced optical technologies based on new plasmonics devices
· highly engineered systems for molecular translocation / movement control
· new molecular designs for enhanced FRET spectroscopy rulers
· integration of the above technologies within a core multifunctional platform and
· iterative refinement of the data analysis based on assessment of instrument performance in SMS, both of nucleic acids
and amino acids.
Long term vision: the technological advances brought about by PROSEQO will:
· make available novel technologies for third generation sequencing of protein and DNA/RNA
· contribute to our understanding of complex biological networks
· significantly advance proteomics research and biomarker development
· facilitate the development of new tools to characterize heterogeneous molecular ensembles through accurate and fast
sequencing
The work performed during this first period represent valuable results for the activities started for the second year. From now on, single molecule translocation and detection experiments will be setup by using the nanostructures realized during this period. Moreover, once the most suitable FRET strategy will be defined, the functionalization strategy defined during the first year will be exploited for optical spectroscopic detection of single molecules. The movement of these latter will be controlled by means of optical trapping thanks to the work now ongoing in UBE.
The expected impact of PROSEQO is to initiate a radically new line of technologies by establishing the proof-of-concept of a NGS methodology.
PROSEQO constitutes an early stage, high risk paradigm of visionary science and is based upon the collaborative research efforts of different participants, highly competent in their fields and motivated to a successful exploration of new founding principles.
The novel multi and interdisciplinary approach of PROSEQO has a large potential to lead to groundbreaking changes in complete proteome mapping, which will provide enormous benefits to the healthcare sector. Indeed, the early stage identification of a disease-specific marker can greatly improve the clinical success rate and reduce mortality, notably in proteinprone diseases such as neurodegenerative and protein-aggregation diseases