Public health concerns have an impact for agri-food industry and so, Sewage Sludge Directive, SSD, includes standard values for pollutants to prevent the contamination of soil and not to lead to adverse effects on ecosystem and public. Recent studies showed that there is no need to revise the directive to include organic contaminants such as diclofenac, since they do not pose any considerable risk. However, risk assessment for nanomaterials is lacking. Silver nanoparticles (AgNPs) are among the most crucial nanomaterials with the production amount of 452 tons/year and 200 tons/year end up in wastewater treatment plants (WWTPs) , almost (>90%), in biosolids. Land spread of biosolids lead to the transfer of AgNPs and its transformation products to the soil environment. Therefore, addressing the fate and toxicity of AgNPs and its transformation products in the soil is essential for Environmental risk assessment (ERA). It is important to know the possible risks before a serious danger occurs for society when AgNPs are being widely used. The objectives of TESNinSAB project were including to understand the fate and effect of AgNPs and their transformation product Ag2SNP by considering the biosolid application in agricultural lands, the transformation ratio of AgNP, the organic matter content of soil and the aging of AgNPs in the environment.