Cel Regeneration of bone defects remains a critical challenge in orthopaedics. Mesenchymal stromal cells (MSC) with biomaterials show huge promise for bone regeneration. However, MSC die shortly after implantation and act as mediators, by secretion of paracrine factors (PF), rather than effectors of bone formation. Importantly, it seems that delivery of cells themselves may not be required for therapeutic benefit. When MSC are cultured in vitro they release PF into their conditioned media (MSC-CM) including cytokines and extracellular vesicles. The goal of this project is to prepare novel biomaterials which are loaded with MSC-CM for in situ bone tissue engineering. PF secreted into MSC-CM during normoxia, hypoxia, and cell death will be measured. Biomaterials (biphasic calcium phosphate ceramics) will be functionalized with MSC-CM by using the polyelectrolyte multi-layering (PEM) method. The biocompatibility, release kinetics, and potential of MSC-CM loaded biomaterials for bone regeneration will be tested in vitro on cells involved in bone formation (MSC, monocytes, osteoclasts, macrophages, and endothelial cells) and in vivo by implanting the biomaterials in subcutis sites and segmented femoral defects in nude mice. Importantly, the delivery of MSC-CM can overcome the donor-dependent variability in bone formation associated with MSC cell therapy and permits a more straightforward transfer of this therapy to clinical treatment. Since MSC-CM is devoid of cells and doesn’t carry patient rejection risk, autologous MSC are not required. Therefore, selected MSC that successfully induce bone formation can be used to collect potent MSC-CM which can be loaded onto biomaterials for therapeutic use in countless patients. An ‘off-the-shelf’ product that could harness the benefits of MSC therapy but circumvent the costly and time consuming multi-step procedures involved with MSC implantation would be of immense interest to the bone regeneration field. Dziedzina nauki natural sciencesbiological sciencescell biologymedical and health sciencesmedical biotechnologytissue engineeringmedical and health sciencesclinical medicineorthopaedicsengineering and technologyindustrial biotechnologybiomaterialsmedical and health sciencesmedical biotechnologycells technologies Program(-y) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Temat(-y) MSCA-IF-2015-GF - Marie Skłodowska-Curie Individual Fellowships (IF-GF) Zaproszenie do składania wniosków H2020-MSCA-IF-2015 Zobacz inne projekty w ramach tego zaproszenia System finansowania MSCA-IF-GF - Global Fellowships Koordynator INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Wkład UE netto € 246 668,40 Adres RUE DE TOLBIAC 101 75654 Paris Francja Zobacz na mapie Region Ile-de-France Ile-de-France Paris Rodzaj działalności Research Organisations Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Uczestnictwo w unijnych programach w zakresie badań i innowacji Opens in new window sieć współpracy HORIZON Opens in new window Koszt całkowity € 246 668,40 Partnerzy (1) Sortuj alfabetycznie Sortuj według wkładu UE netto Rozwiń wszystko Zwiń wszystko Partner Organizacje partnerskie biorą udział w realizacji działania, jednak nie podpisują umowy o grant. PRESIDENT AND FELLOWS OF HARVARD COLLEGE Stany Zjednoczone Wkład UE netto € 0,00 Adres MASSACHUSETTS AVENUE 1350 02138 Cambridge Zobacz na mapie Rodzaj działalności Higher or Secondary Education Establishments Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Uczestnictwo w unijnych programach w zakresie badań i innowacji Opens in new window sieć współpracy HORIZON Opens in new window Koszt całkowity € 160 130,40
