CORDIS oferuje możliwość skorzystania z odnośników do publicznie dostępnych publikacji i rezultatów projektów realizowanych w ramach programów ramowych HORYZONT.
Odnośniki do rezultatów i publikacji związanych z poszczególnymi projektami 7PR, a także odnośniki do niektórych konkretnych kategorii wyników, takich jak zbiory danych i oprogramowanie, są dynamicznie pobierane z systemu OpenAIRE .
Rezultaty
An enzyme engineering programme, combined with newly developed activity assays, will be run to generate insight into structure-function relationships and to, eventually, create better industrial biocatalysts.
Evaluation of most promising PQS-cleaving dioxygenases for use in quorum sensing interference (odnośnik otworzy się w nowym oknie)Generated mutant enzymes will have been tested as improved alternatives in quorum sensing.
High-resolution crystal structures of HPP oxygenase complexed with substrate or inhibitors (odnośnik otworzy się w nowym oknie)By X-ray diffraction, high resolution structures will be determined.
Cofactor-independent oxygenases with few new functions identified within the tautomerase superfamily (odnośnik otworzy się w nowym oknie)Identification and functional characterisation of new cofactor-independent oxygenases within the tautomerase superfamily, with special focus on hypothetical oxygenases for which the genomic context gives clues about their physiological function.
High-resolution crystal structure of the most promising tautomerase superfamily oxygenase (odnośnik otworzy się w nowym oknie)Crystal structures of mutant oxygenases will be obtained with the aim to better understand mechanistic properties of such oxygenases.
Effective biotechnological process for producing indigoid compounds using flavin-dependent monooxygenases (odnośnik otworzy się w nowym oknie)Biotechnological exploration of flavin-dependent monooxygenases for the production of indigoid dyes and application testing for developing a novel process for (in-situ) textile dyeing.
Development of a heterocycle hydroxylation kit (odnośnik otworzy się w nowym oknie)Production of the generated heme-dependent monoxygeneases for formulation as lyophilisates in 96 well plates to develop novel metabolite test kits for the hydroxylation of heterocyclic compounds which are key intermediates for active pharmaceutical intermediates (API) synthesis
Isolated and characterised hydroxylated heterocycles in multi mg to g scale (odnośnik otworzy się w nowym oknie)Using the generated monooxygenases, biocatalytic conversions of heterocycles will be performed, and the formed products will be isolated and characterized.
Flavin-dependent monooxygenases produced and purified (odnośnik otworzy się w nowym oknie)The work will focus on eukaryotic flavin-containing monooxygenases that are essential for the detoxification of xenobiotics. The target enzymes will be produced and purified using protein chromatography.
Identification of at least 2, preferably 5 new hydroxylating heme-dependent monooxygenases (odnośnik otworzy się w nowym oknie)Novel heterocycle hydroxylating heme-dependent monooxygenases: novel enzymes will be identified by in silico and functional screening of plant and fungal transcriptomes.
Identification of amino acid positions that govern selectivity of three heme-dependent monooxygenases (odnośnik otworzy się w nowym oknie)Investigation of three heme-dependent monooxygenases for the conversion of heterocyclic compounds. Through modelling and random mutagenesis positions will be identified and mutated to elucidate on the molecular level structure-function relationships.
Protocol for evaluating (improved) monooxygenases for industrially relevant conversions (odnośnik otworzy się w nowym oknie)Laboratory data will be used to optimise processes in real (pilot-scale) industrial bioreactors, where process conditions such as how oxygen availability affects operational LPMO performance and the overall outcome of biomass conversion processes will be assessed.
Three simultaneous saturation mutagenesis libraries per monooxygenase (odnośnik otworzy się w nowym oknie)The studies monooxygenases will be subjected to multi-site mutagenesis. Three libraries will be prepared.
Crystal structures of flavin-dependent monooxygenases (odnośnik otworzy się w nowym oknie)Elucidation of crystal structures of eukaryotic flavin-containing monooxygenases and analysis of the structural featues in order to understand enzyme-based catalysis.
Identification of substrates and inhibitors of HPP oxygenase (odnośnik otworzy się w nowym oknie)In-depth mechanistic and structural studies of a cofactor-independent monooxygenase from the tautomerase superfamily that catalyses the oxidative cleavage of HPP.
Libraries of enzyme mutants prepared (odnośnik otworzy się w nowym oknie)Generate knowledge-based focussed enzyme mutant libraries
A SERS-based method for the assaying of LPMO activity on a range of polysaccharide substrates (odnośnik otworzy się w nowym oknie)Insight into the mechanism of oxygen activation by the copper active site of LPMOs will be gained and a wholly new assay for the activity of new LPMOs will be developed based on Surface-Enhanced Raman spectroscopy (SERS) of polysaccharide-coated nanoparticles.
Identification of improved flavin-dependent monooxygenases (odnośnik otworzy się w nowym oknie)Screening of the generated libraries will result in discovery of improved variants. This directed evolution approach will focus on generating and studying engineered enzymes optimized for conversion of indole-related and pharmaceutical compounds.
A range of new small-molecule copper complexes which can mimic LPMOs (odnośnik otworzy się w nowym oknie)Knowledge-based design of copper complexes will be tested as mimics of LPMOs.
Evaluation of engineered flavin-dependent monooxygenases in industrial settings (odnośnik otworzy się w nowym oknie)The developed enzymes will be tested under industrially relevant conditions. The performance will be compared with existing traditional methods.
PQS-cleaving cofactor-independent dioxygenases purified and functionally characterised (odnośnik otworzy się w nowym oknie)Isolation of new cofactor-independent dioxygenases from the alpha/beta hydrolase fold superfamily active toward the Pseudomonas quinolone signal (PQS), exploiting natural genetic diversity; and functional characterisation of PQS-cleaving enzymes, with a focus on substrate specificity and affinity, catalytic efficiency, and applicability to interfere with quorum sensing.
Using technology of SSB, mutant libraries will be prepared and transfered to the relevant partners.
Libraries of LPMO mutants prepared (odnośnik otworzy się w nowym oknie)Mutants of LPMOs will be prepared and checked for activity.
For identifying ESR candidates, OXYTRAIN vacancies advertised.
Awards of doctoral degrees (odnośnik otworzy się w nowym oknie)Doctoral degrees will be awarded.
Conference organisation (odnośnik otworzy się w nowym oknie)A conference on “Mechanistic and applied aspects of oxygenases” will be organized.
An OXYTRAIN website will be built and launched
Publikacje
Autorzy:
Andrea N. Fabara, Marco W. Fraaije
Opublikowane w:
Applied Microbiology and Biotechnology, Numer 104/3, 2020, Strona(/y) 925-933, ISSN 0175-7598
Wydawca:
Springer Verlag
DOI:
10.1007/s00253-019-10292-5
Autorzy:
Sandra C. Wullich, Alba Arranz San Martín, Susanne Fetzner
Opublikowane w:
Applied and Environmental Microbiology, Numer 86/9, 2020, Strona(/y) 1-12, ISSN 0099-2240
Wydawca:
American Society for Microbiology
DOI:
10.1128/aem.00279-20
Autorzy:
Gustavo de Almeida Santos, Gaurao V. Dhoke, Mehdi D. Davari, Anna Joëlle Ruff, Ulrich Schwaneberg
Opublikowane w:
International Journal of Molecular Sciences, Numer 20/13, 2019, Strona(/y) 3353, ISSN 1422-0067
Wydawca:
Multidisciplinary Digital Publishing Institute (MDPI)
DOI:
10.3390/ijms20133353
Autorzy:
Gaston Courtade, Luisa Ciano, Alessandro Paradisi, Peter J. Lindley, Zarah Forsberg, Morten Sørlie, Reinhard Wimmer, Gideon J. Davies, Vincent G. H. Eijsink, Paul H. Walton, Finn L. Aachmann
Opublikowane w:
Proceedings of the National Academy of Sciences, Numer 117/32, 2020, Strona(/y) 19178-19189, ISSN 0027-8424
Wydawca:
National Academy of Sciences
DOI:
10.1073/pnas.2004277117
Autorzy:
Gautier Bailleul, Callum R. Nicoll, María Laura Mascotti, Andrea Mattevi, Marco W. Fraaije
Opublikowane w:
Journal of Biological Chemistry, Numer 296, 2021, Strona(/y) 100221, ISSN 0021-9258
Wydawca:
American Society for Biochemistry and Molecular Biology Inc.
DOI:
10.1074/jbc.ra120.016297
Autorzy:
F. Calderaro, M. Keser, M. Akeroyd, L. E. Bevers, V. G. H. Eijsink, A. Várnai, M. A. van den Berg
Opublikowane w:
Biotechnology for Biofuels, Numer 13/1, 2020, ISSN 1754-6834
Wydawca:
BMC (part of Springer Nature)
DOI:
10.1186/s13068-020-01836-3
Autorzy:
Nico D. Fessner
Opublikowane w:
ChemCatChem, 2019, ISSN 1867-3880
Wydawca:
Wiley - VCH Verlag GmbH & CO. KGaA
DOI:
10.1002/cctc.201801829
Autorzy:
Nikola Lončar, Filippo Fiorentini, Gautier Bailleul, Simone Savino, Elvira Romero, Andrea Mattevi, Marco W. Fraaije
Opublikowane w:
Applied Microbiology and Biotechnology, Numer 103/4, 2019, Strona(/y) 1755-1764, ISSN 0175-7598
Wydawca:
Springer Verlag
DOI:
10.1007/s00253-018-09579-w
Autorzy:
Callum R. Nicoll, Gautier Bailleul, Filippo Fiorentini, María Laura Mascotti, Marco W. Fraaije, Andrea Mattevi
Opublikowane w:
Nature Structural & Molecular Biology, Numer 27/1, 2020, Strona(/y) 14-24, ISSN 1545-9993
Wydawca:
Nature Publishing Group
DOI:
10.1038/s41594-019-0347-2
Autorzy:
Marie-Cathérine Sigmund, Gerrit J. Poelarends
Opublikowane w:
Nature Catalysis, Numer 3/9, 2020, Strona(/y) 690-702, ISSN 2520-1158
Wydawca:
Springer Nature
DOI:
10.1038/s41929-020-00507-8
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