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The use of mass spectrometry and optical methods to determine the influence of cofactors on the structure and stability of proteins

Cel

This fellowship will use novel instrumentation to define the role of cofactors on the structure and stability of proteins. We have recently adapted a commercially available Synapt ion mobility mass spectrometer to allow it to perform optical measurements on conformer and mass selected ions, in collaboration with Waters Corp. The heart of this instrument is the ion mobility cell, IM-MS measurements are possible in this instrument, as for any Synapt mass spectrometer, but uniquely we are able to trap ions that have been conformer selected, to allow optical measurements in a so called ‘photo SRIG’ (stacked ring ion guide). Such optical measurements can take several forms, including IR-spectroscopy, UV/Vis-spectroscopy, which will provide structural analysis of co-factors and coenzymes, photo-dissociation to sequence the proteins as well as fluorescence to probe global conformations.
Molecular mechanics calculations will generate structures to compare with those determined experimentally. This MC fellow will use this novel instrument to examine the role of cofactors on the conformations of proteins, by making measurements on the cofactor alone, the apo and the holo form of the protein. The MC fellow will be trained in biological mass spectrometry, in optical methods and in molecular mechanics. They will spend a secondment at Waters developing software to best interpret the data from this instrument. This cutting edge interdisciplinary science program is world leading and will train this fellow with a set of skills highly desirable both in academia and in industry.

Dziedzina nauki (EuroSciVoc)

Słowa kluczowe

Program(-y)

Temat(-y)

System finansowania

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

Wyświetl wszystkie projekty finansowane w ramach tego programu finansowania

Zaproszenie do składania wniosków

(odnośnik otworzy się w nowym oknie) H2020-MSCA-IF-2016

Wyświetl wszystkie projekty finansowane w ramach tego zaproszenia

Koordynator

THE UNIVERSITY OF MANCHESTER
Wkład UE netto
€ 195 454,80
Adres
OXFORD ROAD
M13 9PL Manchester
Zjednoczone Królestwo
Region
North West (England) Greater Manchester Manchester
Rodzaj działalności
Higher or Secondary Education Establishments
Linki
Koszt całkowity
€ 195 454,80