Periodic Reporting for period 3 - INTERACT (Phylogenetic association mapping and its application to secondary metabolite variation in Brassicaceae species)
Okres sprawozdawczy: 2022-09-01 do 2024-02-29
During the past years, great progress has been made in connecting phenotypes to genotypes based on within- species variation. However, the more dramatic variation that can be found between species has not been explored for phenotype/genotype associations so-far. Using classical genetics to mine between-species variation is mostly impossible, because crosses between distinct species hardly work and their genomes are usually highly rearranged.
The goal of this project is to develop genomics-based methods to find association signals in highly re-arranged genomes of different species. To ensure that these methods are also useful in practice, we will apply them to the variation within the Brassicaceae plant family. Given the great potential of genomic technologies in general, our work promises to be ground-breaking and have profound impact on many different fields of genetic research.
The goal of this project is to develop genomics-based methods to find association signals in highly re-arranged genomes of different species. To ensure that these methods are also useful in practice, we will apply them to the variation within the Brassicaceae plant family. Given the great potential of genomic technologies in general, our work promises to be ground-breaking and have profound impact on many different fields of genetic research.
We establish a panel of around 120 diverse Brassicaceae species covering the entire phylogenetic breadth of the Brassicaceae family and build up seed stocks that can be used for inbreeding and phenotyping. A selected subset of these species was whole-genome sequenced and assembled using various different technologies. Gene were annotated.
Developing methods for comparative genomics is needed more than ever in the times where sequencing and assembling a genome is becoming more and more routine. We develop unprecendented methods to associate the phenotypic variation to the difference species. Our work promises ground-breaking insights into evolution, speciation and have profound impact in our understand of the evolution of genetics itself.