A synergistic approach toward understanding receptor signaling in the cell at very high resolution

Informacje na temat projektu

HighResCells

Identyfikator umowy o grant: 810057

DOI

10.3030/810057

Projekt został zamknięty

Data podpisania przez KE 24 Stycznia 2019

Data rozpoczęcia 1 Marca 2019

Data zakończenia 30 Listopada 2024

Finansowanie w ramach

• EXCELLENT SCIENCE - European Research Council (ERC)

Koszt całkowity

€ 8 273 457,00

Wkład UE

€ 8 273 457,00

Koordynowany przez

UNIVERSITAT ZURICH
Switzerland

CORDIS oferuje możliwość skorzystania z odnośników do publicznie dostępnych publikacji i rezultatów projektów realizowanych w ramach programów ramowych HORYZONT.

Odnośniki do rezultatów i publikacji związanych z poszczególnymi projektami 7PR, a także odnośniki do niektórych konkretnych kategorii wyników, takich jak zbiory danych i oprogramowanie, są dynamicznie pobierane z systemu OpenAIRE .

Publikacje

Peer reviewed articles (46)

Gold nanomaterials and their potential use as cryo-electron tomography labels

Autorzy: Charlie Beales; Ohad Medalia
Opublikowane w: Crossref, Numer 8, 2022, ISSN 1047-8477
Wydawca: Academic Press
DOI: 10.1016/j.jsb.2022.107880

Approximating deformation fields for the analysis of continuous heterogeneity of biological macromolecules by 3D Zernike polynomials

Autorzy: Herreros, D; Lederman, RR; Krieger, J; Jimenez-Moreno, A; Martinez, M; Myska, D; Strelak, D; Filipovic, J; Bahar, I; Carazo, JM; Sanchez, COS
Opublikowane w: IUCrJ, Numer Volume 8| Part 6| November 2021| Pages 992-1005, 2021, ISSN 2052-2525
Wydawca: IUCRJ
DOI: 10.1107/s2052252521008903

A network of mixed actin polarity in the leading edge of spreading cells.

Autorzy: Wen-Lu Chung, Matthias Eibauer, Wenhong Li, Rajaa Boujemaa-Paterski, Benjamin Geiger , Ohad Medalia
Opublikowane w: Communications Biology, 2022, ISSN 2399-3642
Wydawca: Springer Nature
DOI: 10.1038/s42003-022-04288-7

Structural analysis of receptors and actin polarity in platelet protrusions

Autorzy: Sorrentino S, Conesa JJ, Cuervo A, Melero R, Martins B, Fernandez-Gimenez E, de Isidro-Gomez FP, de la Morena J, Studt JD, Sorzano COS, Eibauer M, Carazo JM, Medalia O.
Opublikowane w: PNAS Vol. 118 | No. 37, 2021, ISSN 1091-6490
Wydawca: PNAS
DOI: 10.1073/pnas.2105004118

Differential cellular responses to adhesive interactions with galectin-8- and fibronectin-coated substrates

Autorzy: Li W, Sancho A, Chung WL, Vinik Y, Groll J, Zick Y, Medalia O, Bershadsky AD, Geiger B.
Opublikowane w: Journal of Cell Science, 2021, ISSN 0021-9533
Wydawca: The Company of Biologists Ltd.
DOI: 10.1242/jcs.252221

One-Tip enables comprehensive proteome coverage in minimal cells and single zygotes

Autorzy: Zilu Ye, Pierre Sabatier, Javier Martin-Gonzalez, Akihiro Eguchi, Maico Lechner, Ole Østergaard, Jingsheng Xie, Yuan Guo, Lesley Schultz, Rafaela Truffer, Dorte B. Bekker-Jensen, Nicolai Bache, Jesper V. Olsen
Opublikowane w: Nature Communications, Numer 15, 2024, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-024-46777-9

A deep learning approach to the automatic detection of alignment errors in cryo-electron tomographic reconstructions

Autorzy: F.P. de Isidro-Gómez, J.L. Vilas, P. Losana, J.M. Carazo, C.O.S. Sorzano
Opublikowane w: Journal of Structural Biology, Numer 216, 2024, Strona(/y) 108056, ISSN 1047-8477
Wydawca: Academic Press
DOI: 10.1016/j.jsb.2023.108056

Differential ultracentrifugation enables deep plasma proteomics through enrichment of extracellular vesicles

Autorzy: Kverneland, AH, Østergaard O, Emdal KB, Svane IM, Olsen JV
Opublikowane w: Proteomics, 2022, ISSN 1615-9853
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/pmic.202200039

ScipionTomo: Towards Cryo-Electron Tomography Software Integration, Reproducibility, and Validation

Autorzy: J. Jiménez de la Morena, P. Conesa, Y.C. Fonseca, F.P. de Isidro-Gómez, D. Herreros, E. Fernández-Giménez, D. Strelak, E. Moebel, T.O. Buchholz, F. Jug, A. Martinez-Sanchez, M. Harastani, S. Jonic, J.J. Conesa, A. Cuervo, P. Losana, I. Sánchez, M. Iceta, L. del Cano, M. Gragera, R. Melero, G. Sharov, D. Castaño-Díez, A. Koster, J.G. Piccirillo, J.L. Vilas, J. Otón, R. Marabini, C.O.S. Sorz
Opublikowane w: Journal of Structural Biology, 2022, ISSN 1047-8477
Wydawca: Academic Press
DOI: 10.1016/j.jsb.2022.107872

BIPSPI+: Mining Type-Specific Datasets of Protein Complexes to Improve Protein Binding Site Prediction

Autorzy: R. Sanchez-Garcia, J.R. Macias, C.O.S. Sorzano, J.M. Carazo, J. Segura
Opublikowane w: Journal of Molecular Biology, 2022, ISSN 0022-2836
Wydawca: Academic Press
DOI: 10.1016/j.jmb.2022.167556

Local defocus estimation in single particle analysis in cryo-electron microscopy.

Autorzy: Fernandez-Gimenez, E; Carazo, J M; Sorzano, C O S
Opublikowane w: Journal of Structural Biology, 2023, ISSN 1047-8477
Wydawca: Academic Press
DOI: 10.1016/j.jsb.2023.108030

Real-space heterogeneous reconstruction, refinement, and disentanglement of CryoEM conformational states with HetSIREN

Autorzy: D. Herreros, C.P. Mata, C. Noddings, D. Irene, J. Krieger, D.A. Agard, M.-D. Tsai, C.O.S. Sorzano, J.M. Carazo
Opublikowane w: bioRxiv, 2024, ISSN 2692-8205
Wydawca: Cold Spring Harbor Laboratory
DOI: 10.1101/2024.09.16.613176

FSC-Q: a CryoEM map-to-atomic model quality validation based on the local Fourier shell correlation.

Autorzy: Erney Ramírez-Aportela; David Maluenda; Yunior C. Fonseca; Pablo Conesa; Roberto Marabini; J. Bernard Heymann; José María Carazo; C.O.S. Sorzano
Opublikowane w: Nature Communications, Numer 4, 2021, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-020-20295-w

Proteomic investigation of Cbl and Cbl-b in neuroblastoma cell differentiation highlights roles for SHP-2 and CDK16

Autorzy: Pedersen, A.K., Pfeiffer, A., Karemore, G., Akimov, V., Bekker-Jensen, D.B., Blagoev, B., Francavilla, C., and Olsen, J.V.
Opublikowane w: iScience, Volume 24, Numer 4,, 2021, ISSN 0092-8674
Wydawca: Cell Press
DOI: 10.1016/j.isci.2021.102321

Phosphoproteomic investigation of targets of protein phosphatases in EGFR signaling

Autorzy: Akihiro Eguchi, Jesper V. Olsen
Opublikowane w: Scientific Reports, Numer 14, 2024, ISSN 2045-2322
Wydawca: Nature Publishing Group
DOI: 10.1038/s41598-024-58619-1

Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition

Autorzy: Ulises H. Guzman, Ana Martinez-Val, Zilu Ye, Eugen Damoc, Tabiwang N. Arrey, Anna Pashkova, Santosh Renuse, Eduard Denisov, Johannes Petzoldt, Amelia C. Peterson, Florian Harking, Ole Østergaard, Rasmus Rydbirk, Susana Aznar, Hamish Stewart, Yue Xuan, Daniel Hermanson, Stevan Horning, Christian Hock, Alexander Makarov, Vlad Zabrouskov, Jesper V. Olsen
Opublikowane w: Nature Biotechnology, Numer 42, 2024, Strona(/y) 1855-1866, ISSN 1087-0156
Wydawca: Nature Publishing Group
DOI: 10.1038/s41587-023-02099-7

Protein dynamics developments for the large scale and cryoEM: case study of <i>ProDy</i> 2.0

Autorzy: Ivet Bahar; James Krieger; Carlos Oscar S. Sorzano; Jose Maria Carazo Garcia
Opublikowane w: Crossref, Numer 8, 2022, ISSN 1047-8477
Wydawca: Academic Press
DOI: 10.1107/s2059798322001966

Hybrid-DIA: intelligent data acquisition integrates targeted and discovery proteomics to analyze phospho-signaling in single spheroids

Autorzy: Ana Martínez-Val, Kyle Fort, Claire Koenig, Leander Van der Hoeven, Giulia Franciosa, Thomas Moehring, Yasushi Ishihama, Yu-Ju Chen, Alexander Makarov, Yue Xuan, Jesper V Olsen
Opublikowane w: Nature Communications, 2022, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-023-39347-y

Proteomics of resistance to Notch1 inhibition in acute lymphoblastic leukemia reveals targetable kinase signatures

Autorzy: Franciosa, G., Smits, J.G.A., Minuzzo, S., Martinez-Val, A., Indraccolo, S., and Olsen, J.V.
Opublikowane w: Nature communications, 2021, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-021-22787-9

Real-time correction of chromatic aberration in optical fluorescence microscopy

Autorzy: Ana Cayuela López, Pablo Conesa, Ana María Oña Blanco, José Antonio Gómez-Pedrero, Carlos Oscar S Sorzano
Opublikowane w: Methods and Applications in Fluorescence, Numer 11, 2023, Strona(/y) 045001, ISSN 2050-6120
Wydawca: bioRxiv
DOI: 10.1088/2050-6120/ace153

On bias, variance, overfitting, gold standard and consensus in single-particle analysis by cryo-electron microscopy

Autorzy: C. O. S. Sorzano, A. Jiménez-Moreno, D. Maluenda, M. Martínez, E. Ramírez-Aportela, J. Krieger, R. Melero, A. Cuervo, J. Conesa, J. Filipovic, P. Conesa, L. del Caño, Y. C. Fonseca, J. Jiménez-de la Morena, P. Losana, R. Sánchez-García, D. Strelak,E. Fernández-Giménez, F. P. de Isidro-Gómez, D. Herreros, J. L. Vilas, R. Marabinid and J. M. Carazo
Opublikowane w: Acta Crystallographica Section D: Structural Biology, 2022, ISSN 1047-8477
Wydawca: Academic Press
DOI: 10.1107/s2059798322001978

FlexAlign: An Accurate and Fast Algorithm for Movie Alignment in Cryo-Electron Microscopy

Autorzy: David Střelák, Jiří Filipovič, Amaya Jiménez-Moreno, Jose Carazo, Carlos Sánchez Sorzano
Opublikowane w: Electronics, Numer 9/6, 2020, Strona(/y) 1040, ISSN 2079-9292
Wydawca: Electronics (MDPI)
DOI: 10.3390/electronics9061040

Emerging Themes in CryoEM─Single Particle Analysis Image Processing

Autorzy: Jose Luis Vilas; Jose Maria Carazo; Carlos Oscar S. Sorzano
Opublikowane w: Crossref, Numer 12, 2023, ISSN 1047-8477
Wydawca: Academic Press
DOI: 10.1021/acs.chemrev.1c00850

Principal component analysis is limited to low-resolution analysis in cryoEM

Autorzy: Sorzano, COS; Carazo, JM
Opublikowane w: INT UNION CRYSTALLOGRAPHY - ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2021, ISSN 2059-7983
Wydawca: INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/s2059798321002291

Structural investigation of eukaryotic cells: From the periphery to the interior by cryo-electron tomography

Autorzy: Chunyang Wang; Matthias Wojtynek; Ohad Medalia
Opublikowane w: Crossref, Numer 10, 2023, ISSN 0346-251X
Wydawca: Pergamon Press Ltd.
DOI: 10.1016/j.jbior.2022.100923

Unveiling the polarity of actin filaments by cryo-electron tomography

Autorzy: Bruno Martins, Simona Sorrentino, Wen-Lu Chung, Meltem Tatli, Ohad Medalia, Matthias Eibauer
Opublikowane w: Structure, Volume 29, Numer 5, 6 May 2021, Pages 488-498.e4, 2021, ISSN 0036-8075
Wydawca: American Association for the Advancement of Science
DOI: 10.1016/j.str.2020.12.014

Scipion Flexibility Hub: an integrative framework for advanced analysis of conformational heterogeneity in cryoEM

Autorzy: D. Herreros, J. M. Krieger, Y. Fonseca, P. Conesa, M. Harastani, R. Vuillemot, I. Hamitouche, R. Serrano Gutiérrez, M. Gragera, R. Melero, S. Jonic, J. M. Carazo and C. O. Sorzano
Opublikowane w: Acta Crystallographica Section D: Structural Biology, 2023, ISSN 1047-8477
Wydawca: Academic Press
DOI: 10.1107/s2059798323004497

The Actin Network Interfacing Diverse Integrin-Mediated Adhesions

Autorzy: Geiger B, Boujemaa-Paterski R, Winograd-Katz SE, Balan Venghateri J, Chung WL, Medalia O.
Opublikowane w: Biomolecules, 2023, ISSN 2218-273X
Wydawca: MDPI
DOI: 10.3390/biom13020294

ZART: a novel multiresolution reconstruction algorithm with motion-blur correction for single particle analysis

Autorzy: D. Herreros, J. Kiska, E. Ramírez-Aportela, J. Filipovic, J.M. Carazo, C.O.S. Sorzano
Opublikowane w: Journal of Molecular Biology, 2023, ISSN 0022-2836
Wydawca: Academic Press
DOI: 10.1016/j.jmb.2023.168088

Measurement of local resolution in electron tomography

Autorzy: J.L. Vilas, J. Oton, C. Messaoudi, R. Melero, P. Conesa, E. Ramirez-Aportela, J. Mota, M. Martinez, A. Jimenez, R. Marabini, J.M. Carazo, J. Vargas, C.O.S. Sorzano
Opublikowane w: Journal of Structural Biology: X, Numer 4, 2020, Strona(/y) 100016, ISSN 2590-1524
Wydawca: Journal of Structural Biology: X (Elsevier Inc)
DOI: 10.1016/j.yjsbx.2019.100016

Higher resolution in cryo-EM by the combination of macromolecular prior knowledge and image-processing tools

Autorzy: Erney Ramírez-Aportela; Jose M. Carazo; Carlos Oscar S. Sorzano
Opublikowane w: Crossref, Numer 11, 2022, ISSN 2052-2525
Wydawca: IUCrJ
DOI: 10.1107/s2052252522006959

Structures of neurokinin 1 receptor in complex with Gq and Gs proteins reveal substance P binding mode and unique activation features

Autorzy: Christian Thom, Janosch Ehrenmann, Santiago Vacca, Yann Waltenspühl, Jendrik Schöppe, Ohad Medalia, Andreas Plückthun
Opublikowane w: S C I E N C E A D V A N C E S, 2021, ISSN 2375-2548
Wydawca: American Association for the Advancement of Science’s
DOI: 10.1126/sciadv.abk2872

Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution

Autorzy: Martinez-Val, A., Bekker-Jensen, D.B., Steigerwald, S., Koenig, C., Ostergaard, O., Mehta, A., Tran, T., Sikorski, K., Torres-Vega, E., Kwasniewicz, E., et al.
Opublikowane w: Nature Communications, 2021, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-021-27398-y

Scipion-EM-ProDy: A Graphical Interface for the ProDy Python Package within the Scipion Workflow Engine Enabling Integration of Databases, Simulations and Cryo-Electron Microscopy Image Processing.

Autorzy: Krieger, JM; Sorzano, COS; Carazo, JM
Opublikowane w: International Journal of molecular Science, 2023, ISSN 1422-0067
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms241814245

Measuring local-directional resolution and local anisotropy in cryo-EM maps

Autorzy: Jose Luis Vilas, Hemant D. Tagare, Javier Vargas, Jose Maria Carazo, Carlos Oscar S. Sorzano
Opublikowane w: Nature Communications, Numer 11/1, 2020, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-019-13742-w

Advances in Xmipp for Cryo-Electron Microscopy: From Xmipp to Scipion

Autorzy: Strelak, D; Jimenez-Moreno, A; Vilas, JL; Ramirez-Aportela, E; Sanchez-Garcia, R; Maluenda, D; Vargas, J; Herreros, D; Fernandez-Gimenez, E; de Isidro-Gomez, FP; Horacek, J; Myska, D; Horacek, M; Conesa, P; Fonseca-Reyna, YC; Jimenez, J; Martinez, M; Harastani, M; Jonic, S; Filipovic, J; Marabini, R; Carazo, JM; Sorzano, COS.
Opublikowane w: MOLECULES Special Numer Cryo-EM and Hybrid Methods Development: Current Progress and Future Perspectives), Numer Volume 26 Numer 20, 2021, ISSN 1420-3049
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules26206224

A new algorithm for particle weighted subtraction to decrease signals from unwanted components in single particle analysis

Autorzy: E. Fernández-Giménez, M.M. Martínez, R. Marabini, D. Strelak, R. Sánchez-García, J.M. Carazo, C.O.S. Sorzano
Opublikowane w: Journal of Structural Biology, 2023, ISSN 1047-8477
Wydawca: Academic Press
DOI: 10.1016/j.jsb.2023.108024

Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods

Autorzy: Sorzano, COS; Jimenez-Moreno, A; Maluenda, D; Ramirez-Aportela, E; Martinez, M; Cuervo, A; Melero, R; Conesa, JJ; Sanchez-Garcia, R; Strelak, D; Filipovic, J; Fernandez-Gimenez, E; de Isidro-Gomez, F; Herreros, D; Conesa, P; del Cano, L; Fonseca, Y; de la Morena, JJ; Macias, JR; Losana, P; Marabini, R; Carazo, JM
Opublikowane w: Structural Proteomics pp 257-289, 2021, ISSN 1064-3745
Wydawca: Humana Press, Inc.
DOI: 10.1007/978-1-0716-1406-8_13

Multi-layered proteomics identifies insulin-induced upregulation of the EphA2 receptor via the ERK pathway which is dependent on low IGF1R level

Autorzy: Sarah Hyllekvist Jørgensen, Kristina Bennet Emdal, Anna-Kathrine Pedersen, Lene Nygaard Axelsen, Helene Faustrup Kildegaard, Damien Demozay, Thomas Åskov Pedersen, Mads Grønborg, Rita Slaaby, Peter Kresten Nielsen, Jesper Velgaard Olsen
Opublikowane w: Scientific Reports, Numer 14, 2024, ISSN 2045-2322
Wydawca: Nature Publishing Group
DOI: 10.1038/s41598-024-77817-5

DeepEMhancer: a deep learning solution for cryo-EM volume post-processing

Autorzy: Ruben Sanchez Garcia; Josué Gómez-Blanco; Ana Maria Cuervo; José María Carazo; Carlos Oscar S. Sorzano; Javier Vargas
Opublikowane w: Communications Biology, Numer 2, 2021, ISSN 2399-3642
Wydawca: SpringerNature
DOI: 10.1101/2020.06.12.148296

Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries

Autorzy: Dorte B. Bekker-Jensen, Oliver M. Bernhardt, Alexander Hogrebe, Ana Martinez-Val, Lynn Verbeke, Tejas Gandhi, Christian D. Kelstrup, Lukas Reiter, Jesper V. Olsen
Opublikowane w: Nature Communications, Numer 11/1, 2020, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-020-14609-1

Continuous flexibility analysis of SARS-CoV-2 spike prefusion structures

Autorzy: Roberto Melero, Carlos Oscar S. Sorzano, Brent Foster, José-Luis Vilas, Marta Martínez, Roberto Marabini, Erney Ramírez-Aportela, Ruben Sanchez-Garcia, David Herreros, Laura del Caño, Patricia Losana, Yunior C. Fonseca-Reyna, Pablo Conesa, Daniel Wrapp, Pablo Chacon, Jason S. McLellan, Hemant D. Tagare, Jose-Maria Carazo
Opublikowane w: IUCrJ, Numer 7/6, 2020, ISSN 2052-2525
Wydawca: International Union of Crystallography
DOI: 10.1107/s2052252520012725

Estimating conformational landscapes from Cryo-EM particles by 3D Zernike polynomials

Autorzy: D. Herreros, R. R. Lederman, J. M. Krieger, A. Jiménez-Moreno, M. Martínez, D. Myška, D. Strelak, J. Filipovic, C. O. S. Sorzano & J. M. Carazo
Opublikowane w: Nature Communications, 2023, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-023-35791-y

Deep Proteome Analysis of Cerebrospinal Fluid from Pediatric Patients with Central Nervous System Cancer

Autorzy: Christian Mirian, Ole Østergaard, Maria Thastrup, Signe Modvig, Jon Foss-Skiftesvik, Jane Skjøth-Rasmussen, Marianne Berntsen, Josefine Britze, Alex Christian Yde Nielsen, René Mathiasen, Kjeld Schmiegelow, Jesper Velgaard Olsen
Opublikowane w: Journal of Proteome Research, Numer 23, 2024, Strona(/y) 5048-5063, ISSN 1535-3893
Wydawca: American Chemical Society
DOI: 10.1021/acs.jproteome.4c00471

Cryo-EM density maps adjustment for subtraction, consensus and sharpening.

Autorzy: E. Fernández-Giménez; María Jesús Martínez; Rubén J. Sánchez-García; Roberto Marabini; Erney Ramírez-Aportela; Pablo Conesa; José María Carazo; Carlos Oscar S. Sorzano
Opublikowane w: Journal of Structural Biology, Numer 5, 2021, ISSN 1047-8477
Wydawca: Academic Press
DOI: 10.1016/j.jsb.2021.107780

Cryo-EM and Single-Particle Analysis with Scipion

Autorzy: Jimenez-Moreno, A; del Cano, L; Martinez, M; Ramirez-Aportela, E; Cuervo, A; Melero, R ; Sanchez-Garcia, R; Strelak, D; Fernandez-Gimenez, E; de Isidro-Gomez, FP; Herreros, D; Conesa, P; Fonseca, Y; Maluenda, D; de la Morena, JJ ; Macias, JR; Losana, P; Marabini, R; Carazo, JM; Sorzano, COS
Opublikowane w: Biochemistry, 2021, ISSN 1940-087X
Wydawca: MYJoVE Corporation
DOI: 10.3791/62261

Non-peer reviewed articles (3)

Spatiotemporal Proteomics Deciphers Functional Selectivity of EGFR ligands

Autorzy: Akihiro Eguchi,  Kristina B. Emdal, Zilu Ye,  Ulises H. Guzmán,  Jesper V. Olsen
Opublikowane w: Biorxiv.org, 2023, ISSN 2692-8205
Wydawca: BioRxiv
DOI: 10.1101/2023.09.13.557663

Structural basis for the activation and ligand recognition of the human oxytocin receptor

Autorzy: Yann Waltenspühl, Janosch Ehrenmann, Santiago Vacca, Christian Thom, Ohad Medalia, Andreas Plückthun
Opublikowane w: Nature Communications, 2022, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-022-31325-0

A Vaccinia-based system for directed evolution of GPCRs in mammalian cells

Autorzy: Christoph Klenk, Maria Scrivens, Anina Niederer, Shuying Shi, Loretta Mueller, Elaine Gersz, Maurice Zauderer, Ernest S. Smith, Ralf Strohner & Andreas Plückthun
Opublikowane w: Nature Communications, 2023, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-023-37191-8