Scientific Achievements
Work Package 1: Single-Locus Regulation
WP1 focuses on understanding the molecular mechanisms underlying promoter sensing, epigenetic memory, and switching for various genomic loci. Experimental and modelling work addressed Polycomb/Trithoax Regulation, transcriptional bursting, analog and digital control mechanisms operating at a single target locus, Xist regulation, and the establishment of heterochromatin.
Work Package 2: Genome-Wide Targeting
WP2 aims to uncover the molecular mechanisms guiding epigenetic regulators to specific genomic loci and how they respond to disruptions in chromatin structure. This involves integrating models that consider DNA sequence, chromatin connectivity, and dynamic binding interactions. Researchers within WP2 explored the impact of DNA sequence on epigenetic regulation and investigated transcription factor search mechanisms within complex chromatin structures.
Work Package 3: 3D Genome Organization
WP3 focuses on deciphering the dynamic changes in 3D genome organization and their implications for development, transcriptional regulation, and disease. By applying polymer physics models and innovative imaging techniques, researchers investigated the thermodynamic mechanisms driving chromosomal architecture. We developed models for 3D genome regulation, discovered regulatory binding sites through machine learning approaches, and developed novel protocols and bioinformatic tools for detection of small non coding RNAs, which are the subject of several patent applications.
Training and Career Development
We enrolled 15 ESRs (early stage researchers), 6 of whom have successfully defended their PhDs in 2023, and 6 of whom are currently finishing up (August 2023). Key network events provided interdisciplinary training and transferable skills. The PEP-NET Retreat (Copenhagen, Sept 2022) was a training highlight, being the first in-person event since the pandemic. This event allowed final-year students to present their research, enhance presentation skills, and receive mentoring on career paths and interdisciplinary aspects of epigenetics.
Dissemination
PEP-NET has resulted in 17 publications (Publihsed or available on BioRxiv) and 6 PhD theses so far (August 2023). The final conference of the project took place in Naples in April 2023. This event included scientific talks, communication training sessions, a career orientation workshop, and social activities. The conference agenda was designed to promote interactive scientific exchange and networking among ESRs, PIs, and external speakers.
Public Engagement
The Public Engagement initiative has established social media accounts to share ESR contributions, network events, and various online resources for schools and interested non- scientists. ESRs also engaged with school students through Skype sessions, art projects, and games to foster curiosity and interest in epigenetics.
https://itn-pep.net/public-engagement/(odnośnik otworzy się w nowym oknie)
